LOCUS       sequence-45568-e       10062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  TITLE     Edward Callaway plasmids
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBOB-synP-HT
FEATURES             Location/Qualifiers
     source          1..10062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3077..3454
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3467..3476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3473..3475,3476..3478,3479..4189)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3473..3475,3476..3478,3479..4189)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3518..3539)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3779..3798)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4126..4147
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4199..4264
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    4868..5456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4921..4941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5339..5350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5863..6043
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6069..6086)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6075..6299
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6345..6773
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6432..6451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6642..6663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6782..6802)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6787..7117
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6968..7103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7030..7049
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7165..7212
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7231..7605
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7735..7856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7772..7791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7826..7845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7905..7921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7905..7921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7918..7940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7929..7945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7953..7959,7960..7977,7978..7983))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7998..8019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8136..8153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8307..8895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8387..8406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9066..9857,9858..9926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9689..9708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9927..10031)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagagga
     3061 tccgctagcc gccaccatgc cagagccagc gaagtctgct cccgccccga aaaagggctc
     3121 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
     3181 ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat
     3241 ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc
     3301 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
     3361 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
     3421 tactaaggcc atcaccaagt acaccagcgc taaggatcca ccggtcgcca ccatggtgag
     3481 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3541 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3601 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3661 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3721 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3781 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3841 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3901 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3961 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     4021 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4081 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4141 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gtagtggtgt
     4201 gaaacagact ttgaattttg accttctcaa gttggcggga gacgtggagt ccaacccagg
     4261 gcccatggcg cggctgctgc ccgcgctgct gctgctgctg ctgcccggta acgtgaccgg
     4321 taacgggtcc ggtaacggtt ctttgtcccg ttgccccccc ggtcagttcc gctgctcgga
     4381 gccgcccggt gcccacgggg agtgttaccc gcaggactgg ctgtgcgacg gacaccccga
     4441 ctgcgacgac gggcgggacg agtggggctg cgggaccagc gcgatccccg cggtgcccac
     4501 ggacaacggc acagaggctc ccactgcccc tgctcctgga cgtgctctgc cagccaggaa
     4561 tcacggccgc atgtggatgc tgatcactgc agtgctcctg tgctgcctgg tagctgtggg
     4621 tggtatcgct gcatggggga agtccaaagc aaaaagcagg tctgacatct tcagtcttga
     4681 aagcgcatcc aaggagctgc tggtgcctga caagagccag gcagacttgt tctcctgagg
     4741 cgcgccgttt aaacctggaa aaacatggag caatcacaag tagcaacaca gcagctacca
     4801 atgctgcttg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     4861 ctcagacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4921 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4981 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5041 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5101 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5161 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5221 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5281 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5341 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5401 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     5461 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5521 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag acaagatatc
     5581 cttgatctgt ggatctacca cacacaaggc tacttccctg attgacagaa ctacacacca
     5641 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     5701 ccagataaga tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     5761 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     5821 gcatttcatc acgtggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     5881 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5941 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6001 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggccgc tttaaacccg
     6061 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     6121 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     6181 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     6241 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6301 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     6361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6481 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct
     6541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat
     6721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     6781 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat
     6841 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7021 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     7081 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7141 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     7201 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     7261 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7321 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7381 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     7441 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     7501 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     7561 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     7621 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7681 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7741 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7801 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7861 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7921 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7981 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8041 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8101 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8161 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8221 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     8521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9121 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9181 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9241 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9301 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9361 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9421 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9481 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9541 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9601 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9661 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9721 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9781 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9841 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9901 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9961 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10021 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
