LOCUS       sequence-455680-       15899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six 
            gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white 
            screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15899)
  AUTHORS   Tan YY, Liew YY, Lee RRQ, Castel B, Chan NM, Wan WL, Zhang Y, Hu D, 
            Chan P, Kim ST, Chae E
  TITLE     Generation of Inheritable A-to-G Transitions Using Adenine Base 
            Editing and NG-PAM Cas9 in Arabidopsis thaliana.
  JOURNAL   Mol Plant Microbe Interact. 2025 Feb;38(1):30-42. doi: 
            10.1094/MPMI-10-24-0127-TA. Epub 2025 Feb 25.
  PUBMED    39585742
REFERENCE   2  (bases 1 to 15899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-10-24-0127-TA"; journalName: "Mol Plant Microbe 
            Interact"; date: "2025-02"; volume: "38"; issue: "1"; pages: "30-42"
COMMENT     Sequence Label: pECNUS4_MP
FEATURES             Location/Qualifiers
     source          1..15899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1200..1895
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     promoter        2362..2540
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2394..2576
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      3176..3428
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4104..4124)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11168..11491)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     11173..11192
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11386..11408
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11400..11417
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11401..11417
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11418..11474
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11487..11503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11487..11503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11500..11522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11511..11527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11535..11541,11542..11559,11560..11565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11580..11601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    11676..11700
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      11788..12498
                     /label=oriV
                     /note="incP origin of replication"
     promoter        12519..12623
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12624..13418
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13589..14177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14078..14097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             14513..15661
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    15807..15831
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgccgaattc ggatccggag ttctagaatg tcgcggaaca aattttaaaa ctaaatccta
       61 aatttttcta attttgttgc caatagtgga tatgtgggcc gtatagaagg aatctattga
      121 aggcccaaac ccatactgac gagcccaaag gttcgttttg cgttttatgt ttcggttcga
      181 tgccaacgcc acattctgag ctaggcaaaa aacaaacgtg tctttgaata gactcctctc
      241 gttaacacat gcagcggctg catggtgacg ccattaacac gtggcctaca attgcatgat
      301 gtctccattg acacgtgact tctcgtctcc tttcttaata tatctaacaa acactcctac
      361 ctcttccaaa atatatacac atctttttga tcaatctctc attcaaaatc tcattctctc
      421 tagtaaacaa gaacaaaaaa atggcggata cagctagagg aacccatcac gatatcatcg
      481 gcagagatca gtacccgatg atgggccgag atcgtgacca gtaccagatg tccggacgag
      541 gatctgacta ctccaagtct aggcagattg ctaaagctgc aactgctgtc acagctggtg
      601 gttccctcct tgttctctcc agccttaccc ttgttggaac tgtcatagct ttgactgttg
      661 caacacctct gctcgttatc ttcagcccaa tccttgtccc ggctctcatc acagttgcac
      721 tcctcatcac cggttttctt tcctctggag ggtttggcat tgccgctata accgttttct
      781 cttggattta caagtaagca cacatttatc atcttacttc ataattttgt gcaatatgtg
      841 catgcatgtg ttgagccagt agctttggat caattttttt ggtcgaataa caaatgtaac
      901 aataagaaat tgcaaattct agggaacatt tggttaacta aatacgaaat ttgacctagc
      961 tagcttgaat gtgtctgtgt atatcatcta tataggtaaa atgcttggta tgatacctat
     1021 tgattgtgaa taggtacgca acgggagagc acccacaggg atcagacaag ttggacagtg
     1081 caaggatgaa gttgggaagc aaagctcagg atctgaaaga cagagctcag tactacggac
     1141 agcaacatac tggtggggaa catgaccgtg accgtactcg tggtggccag cacactacta
     1201 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
     1261 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     1321 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
     1381 gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta
     1441 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gatgggggcg
     1501 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     1561 tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     1621 aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg
     1681 ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca
     1741 agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa
     1801 gaatcaagga ggccgacaaa gaaacctacg tcgagcagca cgaggtggct gtggccagat
     1861 actgcgacct ccctagcaaa ctggggcaca agtgagctta ccccactgat gtcatcgtca
     1921 tagtccaata actccaatgt cggggagtta gtttatgagg aataaagtgt ttagaatttg
     1981 atcaggggga gataataaaa gccgagtttg aatctttttg ttataagtaa tgtttatgtg
     2041 tgtttctata tgttgtcaaa tggtaccatg ttttttttcc tctctttttg taacttgcaa
     2101 gtgttgtgtt gtactttatt tggcttcttt gtaagttggt aacggtggtc tatatatgga
     2161 aaaggtcttg ttttgttaaa cttatgttag ttaactggat tcgtctttaa ccacaaaaag
     2221 ttttcaataa gctacaaatt tagacacgca agccgatgca gtcattagta catatattta
     2281 ttgcaagtga ttacatggca acccaaactt caaaaacagt aggttgctcc atttagtcgc
     2341 tgcaagaatt caagcttgga ggaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2401 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2461 taaggtcact atcagctagc aaatatttct tgtcaaaatg ctccactgac gttccataaa
     2521 ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa tctgcaccgg
     2581 atctggatcg tttcgcatgt ctcctgaaag aaggcctgct gatattagaa gggctactga
     2641 ggctgatatg cctgctgtgt gcactattgt gaatcattac attgagactt ctactgtgaa
     2701 tttcaggact gagcctcaag aaccacaaga gtggactgat gatcttgtga ggcttagaga
     2761 gaggtatcct tggcttgttg ctgaagtgga tggtgaagtg gctggtattg cttatgctgg
     2821 tccttggaag gctaggaatg cttacgattg gactgctgag tctactgttt acgtgtctcc
     2881 taggcatcaa agaactggtc ttggatctac tctttacact catcttttga agtctttgga
     2941 ggctcagggt ttcaagtctg tggttgctgt gattggactt cctaatgatc cttctgtgag
     3001 gatgcatgag gctcttggtt atgctcctag gggaatgctt agagctgctg gtttcaaaca
     3061 tggaaattgg catgatgtgg gattctggca gcttgatttc tctcttcctg ttcctcctag
     3121 acctgttctt cctgtgactg agatttgagc gggactctgg atctagagtc aagcagatcg
     3181 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     3241 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     3301 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     3361 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     3421 actagatccg ctactagaat tcgagctcag cgttgtcaat caattggcaa gtcataaaat
     3481 gcattaaaaa atattttcat actcaactac aaatccatga gtataactat aattataaag
     3541 caatgattag aatctgacaa ggattctgga aaattacata aaggaaagtt cataaatgtc
     3601 taaaacacaa gaggacatac ttgtattcag taacatttgc agcttttcta ggtctgaaaa
     3661 tatatttgtt gcctagtgaa taagcataat ggtacaacta caagtgtttt actcctcata
     3721 ttaacttcgg tcattagagg ccacgatttg acacattttt actcaaaaca aaatgtttgc
     3781 atatctctta taatttcaaa ttcaacacac aacaaataag agaaaaaaca aataatatta
     3841 atttgagaat gaacaaaagg accatatcat tcattaactc ttctccatcc atttccattt
     3901 cacagttcga tagcgaaaac cgaataaaaa acacagtaaa ttacaagcac aacaaatggt
     3961 acaagaaaaa cagttttccc aatgccataa tactcaaact cagtaggatt ctggtgtgtg
     4021 cgcaatgaaa ctgatgcatt gaacttgacg aacgttgtcg aaaccgatga tacgaacgaa
     4081 agcaagcagc gttagccacc tccaaccttc ctcttcttct taggatcagc ccttgaatca
     4141 ccaccgagct gtgagagatc gatcctagtc tcgtagagtc cagtgataga ctgatggatg
     4201 agggtagcat cgagcacttc tttggtagac ctgtacacct tcctatcgat ggttgtatcg
     4261 aagtacttga aagccctagg agcaccgagg ttggtaaggg tgaagagatg gatgatgttc
     4321 tctgcctgtt ccctgatagg cttatctctg tgcttgttgt aagcagacaa caccttatcg
     4381 aggtttgcat cagcgaggat caccctttta gagaactcag agatctgctc gatgatctca
     4441 tccaagtagt gcttgtgctg ctcaacgaaa agttgcttct gctcgttatc ttctggagat
     4501 cccttcaact tctcgtagtg agaagcgagg taaagaaagt taacgtactt agatgggaga
     4561 gcaagctcgt ttcccttttg aaggaaccta gcagaagcga gcatcctctt tctaccgttc
     4621 tcgagttcga agagtgagta ctttgggagc ttgatgatga gatccttctt aacctctttg
     4681 tatcccttag cctcgaggaa atcgattggg ttcttctcga aagatgacct ttccatgata
     4741 gtgattccga gaagttcctt aacagacttg agcttcttac tctttccctt ctcaacctta
     4801 gccacaacga gaacagagta agccacggta ggagacacga aaccaccgta tttcttaggg
     4861 tcccaatcct tcttcctagc aatgagctta tcagagttcc tcttaggcct gatagactct
     4921 ttagagaatc caccggtctg cacctcggtt ttcttaacga tgttcacctg tggcatagag
     4981 agcacctttc taacggtagc gaaatccctt cccttatccc acacgatctc acctgtttca
     5041 ccgtttgtct cgatgagtgg cctctttctg atctcaccgt tagcgagggt aatctcggtc
     5101 ttgaagaaat tcatgatgtt agagtagaag aaatacttag cggtagcctt tccgatctct
     5161 tgctcagact tagcgatcat cttcctcaca tcgtacacct tgtaatcacc gtacacgaac
     5221 tctgactcga gcttaggata cttcttgatg agagcggttc caacaacagc gttaaggtaa
     5281 gcatcgtgag cgtggtggta gttgttgatt tccctcacct tgtagaattg gaaatccttt
     5341 ctgaaatcag acacgagctt tgacttgagg gtgataacct tcacttccct gatcaactta
     5401 tcgttctcat cgtacttggt gttcatccta gaatcgagga tctgtgcaac gtgcttagtg
     5461 atctgcctgg tttccacaag ctgcctcttg atgaatcctg ccttatccaa ttcagagagt
     5521 cctcccctct cagccttagt caagttatcg aactttctct gagtgatgag cttagcgttg
     5581 aggagctgcc tccaatagtt cttcattttc ttcacaacct cttcacttgg cacgttatca
     5641 ctcttacccc tgttcttatc agacctggtg agcaccttgt tatcgataga atcatccttc
     5701 aagaatgact gtggcacgat atgatcaaca tcgtaatcag agagcctgtt gatatccaac
     5761 tcttgatcca catacatatc ccttccgttc tggaggtagt agaggtagag cttctcattc
     5821 tggagctgag tgttctcaac agggtgctct ttgaggatct gagatccaag ctctttgata
     5881 ccttcctcga tcctcttcat cctttcccta gagttcttct gtcccttctg agtggtctgg
     5941 ttctctctag ccatttcgat cacgatgttc tcaggcttat gccttcccat caccttcacc
     6001 aactcatcca caaccttcac agtctggagg attcccttct tgattgcagg agatccagcg
     6061 aggttagcga tatgctcatg gagactatca ccctgtcctg aaacctgagc cttctggata
     6121 tcctctttaa aggtgagaga atcatcgtgg atgagctgca tgaagtttct gttagcgaat
     6181 ccatcagact tgaggaaatc aaggattgtc tttccagact gcttatccct gattccgtta
     6241 atgagctttc ttgagagcct tccccaacca gtgtatcttc ttctcttcaa ctgcttcatc
     6301 accttatcat cgaagagatg agcgtaggtc ttgagccttt cttcaatcat ctctctatct
     6361 tcaaagaggg tgagggtaag aacgatatcc tccaagatat cctcgttttc ctcgttatcc
     6421 aagaaatcct tatccttaat gatcttgagg agatcgtggt aggttccgag agatgcgttg
     6481 aacctatcct caacaccaga aatctcaact gaatcgaagc actcgatttt cttgaagtaa
     6541 tcctctttga gctgcttcac ggtcaccttt ctgttggtct tgaacaagag atcaacgata
     6601 gccttctttt gctcacctga caaaaaagca ggcttcctca ttccctcggt cacgtactta
     6661 accttggtca actcgttgta cacggtgaag tactcgtaga gcaaagagtg cttagggagc
     6721 accttctcgt ttggaaggtt cttatcgaag ttggtcatcc tctcgatgaa agactgagca
     6781 ctagcaccct tatccaccac ctcttcgaag ttccaagggg tgatggtttc ctcagacttt
     6841 ctggtcatcc aagcgaatct tgagtttcct ctagcgagag gtcccacgta gtaagggatt
     6901 ctgaaggtga gaatcttctc aatcttttcc ctgttatcct tgaggaatgg gtagaaatcc
     6961 tcttgccttc taaggatagc gtgcaactct ccgaggtgga tctgatgagg gatagatccg
     7021 ttatcgaagg tcctctgctt tctgagaaga tcctctctat tgagcttcac gaggagttcc
     7081 tcggttccat ccatcttctc gaggataggc ttgatgaact tgtagaactc ttcttgagat
     7141 gcaccaccat cgatgtaacc agcgtatccg ttcttagact gatcgaagaa aatctctttg
     7201 tacttctctg ggagctgctg tctaacaaga gccttgagaa gtgtgagatc ctggtggtgc
     7261 tcatcgtatc tcttgatcat agaagctgag agtggagcct tggtgatctc ggtgttcact
     7321 ctgaggatat cactgaggag gatagcatca gagaggttct tagcagcgag gaacaaatca
     7381 gcgtactgat ctccgatctg agcgaggagg ttatcgagat catcatcgta ggtatccttt
     7441 gagagctgga gctttgcatc ctcagcgaga tcgaagttag acttgaagtt aggggtgagt
     7501 ccgagagaga gagcgatcaa gtttccgaaa agtccgttct tcttctcacc agggagctga
     7561 gcaatgaggt tctcaagcct tcttgactta gagagcctag cagagaggat agccttagca
     7621 tccacacctg aagcgttgat agggttctct tcgaaaagct ggttgtaggt ctgcacgagc
     7681 tggatgaaca acttatccac atcagagtta tcagggttga gatcaccctc gatgaggaag
     7741 tgtcctctga acttgatcat gtgagcgaga gcgaggtaga tgagcctgag atcagcctta
     7801 tcagtagaat caacgagctt ctttctgagg tggtagatag tagggtactt ctcgtggtat
     7861 gccacctcat caacgatgtt tccgaagata gggtgcctct cgtgcttctt atcttcttcc
     7921 acgaggaatg actcttcgag cctgtggaag aatgaatcat ccactttagc catctcgtta
     7981 gagaagatct cttggaggta gcagatcctg ttctttcttc tggtgtacct tcttctagcg
     8041 gttctcttga gtctggtagc ctcagcagtt tcaccagaat cgaagaggag agcaccgata
     8101 aggtttttct tgatagagtg cctatcggtg tttccgagaa ccttgaactt cttagatggc
     8161 accttgtact catcggtgat cacagcccat cccacagagt tagttccgat ggcgagtccg
     8221 atagagtact tcttatccat acttccacca gatgatcctc cagatgattc aggggtagca
     8281 ctctcagatg ttccaggcgt ttcagaacca gaagaacctc cagaagaacc tccagaatta
     8341 atagaagatt gagctttctt ttgagcattg aaaacttgtc ttggcattcg gtaaaaatca
     8401 cacaaaagag cagcacattc atcagccaag ataccctctg taatttcaac tctatgattc
     8461 ataccaggat agttcaaaac attcataaga gaaccagcag ctcctctctt agaatttcta
     8521 acaccgaaaa caactcttcc gattcttgaa tgaatcatag ctccagcaca cataacacat
     8581 ggttcgaatg taacataaag agtagcatcg ataagtctat agttctgcat aaccaaacct
     8641 ccttgtctaa gagccataat ctcagcatga gcagtaggat catgaagtcc gatagctcta
     8701 ttccaacctt ctccaataac tctgttattc aaaacaagaa cagctccaac aggaacctct
     8761 ctttcatccc ttgccctttt tgctaatgta agtgcgtgcc tcatccagta ttcgtgacta
     8821 aactccacct ctgaagatcc gccgctagac ccaccagacg actctggtgt cgccgactcc
     8881 gacgtgcctg gagtctcaga acctgacgac cccccggagc tgccaccaga atccgtcgat
     8941 gactgtgcct tcttctgcgc tttgatttct tgtctccgca tccggaagaa gtcggaaagc
     9001 agagccgcgc attcatccgc caaaataccc tcggtgattt ccaccctgtg gttcatgcca
     9061 ggatggtgca aaacgtccat aaggctacct gctgcgcctg tttttgcatc cctagcgccg
     9121 aacacgacgc gcccaatgcg ggaatgaatc attgcgcccg cgcacatgac acacggctcg
     9181 agagtcacat aaagagtagc gtctatcaag cgatagttct gcatcaccag acccccctgt
     9241 ctaagagcca taatctcagc atgtgctgtt ggatcgtgtc tgcctatcgg ccgattccac
     9301 ccctctccga taacgcggtt attgtgcaca agcacagccc ccaccggaac ttcccgctcg
     9361 tcccacgctc tcttggccaa cgtcaaagcg tgccgcatcc aatactcgtg ggaaaactca
     9421 acttctgaca ttggctgtgg tgagagaaac agagcgtgag ctcaaataca caagtacgaa
     9481 atcagagtta tgagagagga tttggagaaa gaagatcacc tgaagatcgt ttgcgtcgcg
     9541 cgaatgagag agacggcaga gaaaacgaga agctttaaga ggacgaacaa aaaccctaat
     9601 gtcactcctt ttatatagat gagaaattta gggtttgggc tctaatggag ctgtctacat
     9661 gggctttcat ttctttatcc agtaaagaac cggtttaaca ggttccctgg tttatacgtg
     9721 gttttagccg gttaacttac ggaatattat ttttatctta agttacacaa attacatcat
     9781 taatagtaaa acaaaatttt atggtgcaat ttaagttttt atgatgagtg aaattcgaat
     9841 aatatacaaa tattttactg tttaggtttc cctaaaccta cttttagata tgtaaattaa
     9901 acattttttt ttttttggtt agaaccaagt cagtgatcat agaactcaaa aagctggtgt
     9961 ttcaattggt acaagcattt ttattgcaag atataaaaat tcagaaggac atatccttga
    10021 tgtatgtaca tgaaggaacc gattttgact ccaagctaca aagaagaaga agaatgatat
    10081 acaaagaaaa ctgcatatcc tcaccatgaa attgaaagct agaggaaggg gaataacaga
    10141 tactctgcgt agaagagatt ccatataaac cgataatatc ctgagatagc ttcctgcaat
    10201 aatcaaatgt ttctacactc cgttagatat acactccatt gatgttccgt gataacgagt
    10261 ttatgtttgt acaaaagtat tttaccttgg tgtagtttgc tttttctagt acccatgtct
    10321 accaaagatc aacaacaaag atattagaga ttaatgaatt tggcagtgtc aaatgcagaa
    10381 actaaagcat atagttatga agttcttgtg cattatctca aaagtatgta agagatataa
    10441 gagtatctca taatgcttta cctggaaaat taagcctgaa gccaagatca catgtgcagg
    10501 aatcatcaag ctacctctaa aaacctgttc attgttaaag gataagaggg ttggtctttt
    10561 caaagctgca tactgaggaa agtaacaata tcgcaacaaa aataaattgg tggcgatagt
    10621 ttatcaagct tctacatgca gtaatggtgt tcgtgcttta attaaccgaa atagttaaga
    10681 cggaaataga tgaattcacc tgaggcatgt agaaagctag tgatatggct gaaacataat
    10741 ttactagcag aagtccagaa ccgaggaatg caatgtttct cactccaagt tttgttgcca
    10801 gggttgatat ttggaaccta ttcaagatca atcaaaggca tgattaacta aacatgcttg
    10861 aacaaacaca taaagacaat atatcaaacg gagctggtta aacattgtct ttgactgtca
    10921 aatggattga gccaaagagc ggaaagaaaa tggttatgcg tgcagttact tactttcgat
    10981 ctccttcaac atcaggaagg tcctttgtaa tagcaatgac cagtgcaaac agtgtcacaa
    11041 aagatgtgat gaacgccaca ggtgcactgc aaatagcgaa tcaaaagttg agtaatatat
    11101 ctactcacga ttatggggag ctccttacga attccactag aattcgagct cggagtgcca
    11161 agtcttccta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    11221 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
    11281 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    11341 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    11401 gtaaaacgac ggccagtgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
    11461 aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    11521 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    11581 tgagtgagct aactcacatt agaagacaag ggagctttac gaggatgcac atgtgaccga
    11641 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
    11701 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
    11761 tccgttcgtc catttgtatg tgccagccgc ctttgcgacg ctcaccgggc tggttgccct
    11821 cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc
    11881 gtgtgcgaga caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac
    11941 tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac
    12001 agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg
    12061 cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag
    12121 tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt
    12181 gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct
    12241 gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta
    12301 acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg
    12361 cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc
    12421 ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc
    12481 tcaccccaaa aatggcagcg ctggccaatt cccgagtgcg cggaacccct atttgtttat
    12541 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    12601 aataatattg aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac
    12661 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
    12721 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
    12781 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
    12841 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
    12901 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
    12961 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
    13021 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
    13081 atgtggattg cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg
    13141 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
    13201 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
    13261 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgctcagg gaggatatcg
    13321 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
    13381 aaataaaata ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat
    13441 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    13501 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    13561 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    13621 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    13681 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    13741 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    13801 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    13861 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    13921 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    13981 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    14041 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    14101 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    14161 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gcagatccta
    14221 gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc gacttcttcc gcatcaagtg
    14281 ttttggctct caggccgagg cccacggcaa gtatttgggc aaggggtcgc tggtattcgt
    14341 gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct acgggaccga
    14401 cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt caaatcagga
    14461 ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg
    14521 gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt ccgccgagga
    14581 tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt
    14641 cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc aactggctcc
    14701 ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtcttg aacaggaggc
    14761 ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga ccaagaagcg
    14821 aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct
    14881 gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg
    14941 gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt tcaccacgcg
    15001 caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg tcaacaagga
    15061 cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac tggtgtggca
    15121 gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct tcacgttcta
    15181 cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga aggccgagga
    15241 atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg ttgggcacct
    15301 ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga aaacgtcccg
    15361 ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc actacacgaa
    15421 attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt tcgactattt
    15481 cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca tgtgcggatc
    15541 ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg
    15601 aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta
    15661 gggccttgtg gggtcagttc cggctggggg ttcagcagcc cctgctcgga tctgttggac
    15721 cggacagtag tcatggttga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
    15781 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
    15841 cttagacaac ttaataacac attgcggacg tttttaatgt actggggttg aacactctg
//
