LOCUS       sequence-455811-       11625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing mitochondrion outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: MT3
FEATURES             Location/Qualifiers
     source          1..11625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..69)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(240..1010)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(join(1014..1724,1725..1727))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(1014..1724,1725..1727))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1056..1077)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1405..1424
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(1740..1766)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1779..1805)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1818..1844)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(1862..2242)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2776..2793)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2776..2792)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2785..2807)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2995..3019
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4320..4949
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5378..6451
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6517..6711
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6947..6969)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7055..7195
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7110..7129
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7210..7227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7381..7969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7461..7480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8056..8850)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9275..9299
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9377..9551
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9592..10617)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10690..11035)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10727..10747)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11226..11247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11262..11267,11268..11285,11286..11292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11300..11316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11305..11327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11324..11340
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11324..11340
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(11357..11609)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ttacttatcg tcatcgtcct tgtaatcgat gtcgtgatcc ttatagtctc catcatggtc
       61 tttgtagtcc atggtaccgg atccactagt atttaaatgt cgaccccgga tccactagtt
      121 cagagctgct ttcggtatct cacgaaggcc caaactgcta ccagggtaag ggcaaacact
      181 ggcagcagca ccacagctac aggaactcca ctaggctctc cgtcgcctcg atgccgcggc
      241 ttttcggcag accgcagact gatttctccg cccatccagg gtttgataac tccgtcctgt
      301 tccagcagca gggctccctg tttatcaatc ccccggctaa tcccgaaaat ctctttgtcc
      361 ccaatgatca gcttcacggg tctattgatg aagttatcca gcttctccca ccgtgacaga
      421 tatggagcca ggccttcctg ctcgaacagt tccagggctg cccgcagctc tcggatcagc
      481 atagcggcca gagtattcct gtccaggtta atccctgctt cctgcagtgt gatccagccc
      541 tgattgacca cgctttcctc cacgcgcctc atagccacgt taatcccggc gccaatgacg
      601 atctgtgcag catcgcctgt tattccggcc agctccacca ggatgcctgc cagctttcta
      661 tcctgcagat acaggtcatt gggccatttg actcgcacct tgtctgctcc cagctttcgc
      721 agcgcttctg ccatgacaat tccgatgacc gggcccaggc cgattgctgc tggtccccgc
      781 ttcaggcgcc aaaacatact caggtacagg ttagctccaa aaggagagaa ccatttcctc
      841 cctctgcttc ctctccctgc ctgctgatat tctgcaatgc aagcatcccc actcttcagc
      901 tcgccgattc gatccagcag gtactgattg gtggagtcga ccacaggcag gactgccacg
      961 ctcccgccgt ccagctgtcc cagaatctgt ttagcgttca gcagcgggat catcttgtac
     1021 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     1081 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     1141 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     1201 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     1261 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     1321 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     1381 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     1441 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     1501 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     1561 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     1621 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     1681 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcacgcc gctccctcca
     1741 gcataatcag gcacatcata agggtatccg gcgctgccag cgtagtcagg cacatcatag
     1801 gggtaggatc ctgtgccggc gtaatcaggc acgtcatagg gatacataag ctttctagag
     1861 tcgatttggt gtatcgagat tggttatgaa attcagatgc tagtgtaatg tattggtaat
     1921 ttgggaagat ataataggaa gcaaggctat ttatccattt ctgaaaaggc gaaatggcgt
     1981 caccgcgagc gtcacgcgca ttccgttctt gctgtaaagc gttgtttggt acacttttga
     2041 ctagcgaggc ttggcgtgtc agcgtatcta ttcaaaagtc gttaatggct gcggatcaag
     2101 aaaaagttgg aatagaaaca gaatacccgc gaaattcagg cccggttgcc atgtcctaca
     2161 cgccgaaata aacgaccaaa ttagtagaaa aataaaaact gactcggata cttacgtcac
     2221 gtcttgcgca ctgatttgaa aaatctccct cgatcgagaa agagatcaat gttgagctgc
     2281 ttcaaaagca atgggattga ccagctcgcg gatcctacag gccaaattcg ctcttagccg
     2341 tacaatatta ctcaccggtg cgatgccccc catcgtaggt gaaggtggaa attaatgatc
     2401 catcttgaga ccacaggccc acaacagcta ccagtttcct caagggtcca ccaaaaacgt
     2461 aagcgcttac gtacatggtc gataagaaaa ggcaatttgt agatgttaac atccaacgtc
     2521 gctttcaggg atcctacagg ccaaattcgc tcttagccgt acaatattac tcaccggtgc
     2581 gatgcccccc atcgtaggtg aaggtggaaa ttaatgatcc atcttgagac cacaggccca
     2641 caacagctac cagtttcctc aagggtccac caaaaacgta agcgcttacg tacatggtcg
     2701 ataagaaaag gcaatttgta gatgttaaca tccaacgtcg ctttcaggga tccgcgagct
     2761 tatcgatacc gtggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2821 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2881 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     2941 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     3001 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     3061 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     3121 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     3181 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     3241 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     3301 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     3361 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     3421 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     3481 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     3541 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     3601 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     3661 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     3721 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     3781 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     3841 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     3901 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     3961 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     4021 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     4081 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     4141 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     4201 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     4261 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     4321 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     4381 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     4441 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     4501 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     4561 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     4621 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     4681 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     4741 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     4801 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     4861 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     4921 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     4981 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     5041 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     5101 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     5161 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     5221 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     5281 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     5341 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     5401 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     5461 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     5521 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     5581 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     5641 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     5701 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     5761 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     5821 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     5881 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     5941 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     6001 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     6061 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     6121 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     6181 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     6241 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     6301 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     6361 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     6421 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     6481 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     6541 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     6601 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     6661 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     6721 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     6781 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     6841 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     6901 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     6961 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7021 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     7081 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     7141 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     7201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7801 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     7861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8041 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
     8101 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
     8161 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
     8221 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
     8281 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
     8341 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
     8401 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
     8461 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
     8521 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
     8581 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
     8641 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
     8701 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
     8761 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
     8821 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
     8881 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
     8941 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
     9001 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt
     9061 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
     9121 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
     9181 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
     9241 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     9301 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg
     9361 ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg aattagaaat
     9421 tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa
     9481 cacatgagcg aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta
     9541 ttatggagaa actcgagctt gtcgatcgac agatcccggt cggcatctac tctatttctt
     9601 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     9661 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     9721 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     9781 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg
     9841 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     9901 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     9961 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
    10021 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
    10081 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
    10141 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
    10201 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
    10261 catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa
    10321 cgtgacaccc tgtgaacggc gggagatgca ataggtcagg ctctcgctaa actccccaat
    10381 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
    10441 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
    10501 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
    10561 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
    10621 tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg
    10681 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
    10741 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
    10801 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    10861 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
    10921 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
    10981 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
    11041 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
    11101 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
    11161 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    11221 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    11281 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    11341 atgattacga attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
    11401 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
    11461 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
    11521 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
    11581 aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctc
//
