LOCUS       sequence-45582-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Dmitriev PV, Vassetzky YS
  TITLE     A set of vectors for introduction of antibiotic resistance genes by 
            in vitro Cre-mediated recombination.
  JOURNAL   BMC Res Notes. 2008 Dec 23;1:135. doi: 10.1186/1756-0500-1-135.
  PUBMED    19105832
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2008-12-23"; volume: "1:135. doi"; pages: " 
            10.1186/1756-0500-1-135"
COMMENT     Sequence Label: pT-TK
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(90..123)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     750..769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
       61 gtttactcat gtcgaggggg ggcccatgga taacttcgta tagcatacat tatacgaagt
      121 tatggcccgg cctcaggatt cttccttttt caatattatt gaagcattta tcagggttat
      181 tgtctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
      241 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
      301 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      361 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      421 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      481 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      541 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      601 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      661 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      721 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      781 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggg
//
