LOCUS       sequence-455820-       11941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing peroxisome outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: PX3
FEATURES             Location/Qualifiers
     source          1..11941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..70)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(205..717,718..720,721..723))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     398..417
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(742..1512)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     promoter        complement(1836..2216)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2969..2986)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2969..2985)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2978..3000)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3188..3212
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4513..5142
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5571..6644
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6710..6904
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7140..7162)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7248..7388
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7303..7322
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7403..7420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7574..8162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7654..7673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8249..9043)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9468..9492
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9570..9744
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9785..10810)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10883..11228)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10920..10940)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11419..11440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11455..11460,11461..11478,11479..11485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11493..11509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11498..11520
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11517..11533
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11517..11533
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(11550..11802)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11822..11848)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(11861..11887)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(11900..11926)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctccttg tacaggtcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
       61 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      121 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      181 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      241 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      301 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      361 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      421 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      481 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
      541 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
      601 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
      661 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
      721 catggtaccg gatccactag tcttttcggc agaccgcaga ctgatttctc cgcccatcca
      781 gggtttgata actccgtcct gttccagcag cagggctccc tgtttatcaa tcccccggct
      841 aatcccgaaa atctctttgt ccccaatgat cagcttcacg ggtctattga tgaagttatc
      901 cagcttctcc caccgtgaca gatatggagc caggccttcc tgctcgaaca gttccagggc
      961 tgcccgcagc tctcggatca gcatagcggc cagagtattc ctgtccaggt taatccctgc
     1021 ttcctgcagt gtgatccagc cctgattgac cacgctttcc tccacgcgcc tcatagccac
     1081 gttaatcccg gcgccaatga cgatctgtgc agcatcgcct gttattccgg ccagctccac
     1141 caggatgcct gccagctttc tatcctgcag atacaggtca ttgggccatt tgactcgcac
     1201 cttgtctgct cccagctttc gcagcgcttc tgccatgaca attccgatga ccgggcccag
     1261 gccgattgct gctggtcccc gcttcaggcg ccaaaacata ctcaggtaca ggttagctcc
     1321 aaaaggagag aaccatttcc tccctctgct tcctctccct gcctgctgat attctgcaat
     1381 gcaagcatcc ccactcttca gctcgccgat tcgatccagc aggtactgat tggtggagtc
     1441 gaccacaggc aggactgcca cgctcccgcc gtccagctgt cccagaatct gtttagcgtt
     1501 cagcagcggg atcatactag tctgagagag aagaatagca gtcagaacct gaagagactt
     1561 caatcctcct cctttcttct ttttcgtggt tatctttgca ttttgatcag ttgcagtcag
     1621 cttttccaaa gtctcatcat catcactctg accattgcaa agacgcgaag aatcaggcct
     1681 ccgggaagcg acccttgatt tcaggtaaac agcagttcca ccagcagcta caatcccagc
     1741 cgcaagcagt atagatttcc gtcttgacgc tacaagaccc cgaccccgct cagttaactg
     1801 caatagttga agtgaaggca taagctttct agagtcgatt tggtgtatcg agattggtta
     1861 tgaaattcag atgctagtgt aatgtattgg taatttggga agatataata ggaagcaagg
     1921 ctatttatcc atttctgaaa aggcgaaatg gcgtcaccgc gagcgtcacg cgcattccgt
     1981 tcttgctgta aagcgttgtt tggtacactt ttgactagcg aggcttggcg tgtcagcgta
     2041 tctattcaaa agtcgttaat ggctgcggat caagaaaaag ttggaataga aacagaatac
     2101 ccgcgaaatt caggcccggt tgccatgtcc tacacgccga aataaacgac caaattagta
     2161 gaaaaataaa aactgactcg gatacttacg tcacgtcttg cgcactgatt tgaaaaatct
     2221 ccctcgatcg agaaagagat caatgttgag ctgcttcaaa agcaatggga ttgaccagct
     2281 cgcggatcct acaggccaaa ttcgctctta gccgtacaat attactcacc ggtgcgatgc
     2341 cccccatcgt aggtgaaggt ggaaattaat gatccatctt gagaccacag gcccacaaca
     2401 gctaccagtt tcctcaaggg tccaccaaaa acgtaagcgc ttacgtacat ggtcgataag
     2461 aaaaggcaat ttgtagatgt taacatccaa cgtcgctttc agggatccta caggccaaat
     2521 tcgctcttag ccgtacaata ttactcaccg gtgcgatgcc ccccatcgta ggtgaaggtg
     2581 gaaattaatg atccatcttg agaccacagg cccacaacag ctaccagttt cctcaagggt
     2641 ccaccaaaaa cgtaagcgct tacgtacatg gtcgataaga aaaggcaatt tgtagatgtt
     2701 aacatccaac gtcgctttca gggatcctac aggccaaatt cgctcttagc cgtacaatat
     2761 tactcaccgg tgcgatgccc cccatcgtag gtgaaggtgg aaattaatga tccatcttga
     2821 gaccacaggc ccacaacagc taccagtttc ctcaagggtc caccaaaaac gtaagcgctt
     2881 acgtacatgg tcgataagaa aaggcaattt gtagatgtta acatccaacg tcgctttcag
     2941 ggatccgcga gcttatcgat accgtggcac tggccgtcgt tttacaacgt cgtgactggg
     3001 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3061 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3121 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat
     3181 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     3241 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     3301 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     3361 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     3421 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     3481 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     3541 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     3601 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     3661 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     3721 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     3781 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     3841 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     3901 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     3961 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     4021 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     4081 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     4141 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     4201 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     4261 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     4321 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     4381 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     4441 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     4501 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     4561 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     4621 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     4681 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     4741 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     4801 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     4861 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     4921 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     4981 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     5041 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     5101 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     5161 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     5221 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     5281 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     5341 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     5401 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     5461 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     5521 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     5581 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     5641 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     5701 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     5761 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     5821 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     5881 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     5941 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     6001 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     6061 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     6121 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     6181 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     6241 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     6301 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     6361 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     6421 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     6481 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     6541 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     6601 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     6661 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     6721 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     6781 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     6841 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     6901 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     6961 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     7021 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     7081 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     7141 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     7201 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     7261 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     7321 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     7381 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7981 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8221 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
     8281 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     8341 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
     8401 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
     8461 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
     8521 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
     8581 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
     8641 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
     8701 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
     8761 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
     8821 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
     8881 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
     8941 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
     9001 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
     9061 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     9121 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
     9181 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
     9241 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
     9301 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
     9361 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
     9421 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
     9481 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
     9541 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
     9601 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
     9661 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac
     9721 tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccc ggtcggcatc
     9781 tactctattt ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     9841 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     9901 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     9961 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
    10021 cccggagtcg tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
    10081 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
    10141 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
    10201 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
    10261 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
    10321 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
    10381 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
    10441 cagcgatcgc atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag
    10501 gcaggtcttg caacgtgaca ccctgtgaac ggcgggagat gcaataggtc aggctctcgc
    10561 taaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
    10621 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
    10681 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
    10741 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
    10801 gctttttcat atctcattgc cccccggatc tgcgaaagct cgagagagat agatttgtag
    10861 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
    10921 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
    10981 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
    11041 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
    11101 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
    11161 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
    11221 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
    11281 gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc
    11341 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    11401 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    11461 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    11521 aaacagctat gacatgatta cgaattcccg atctagtaac atagatgaca ccgcgcgcga
    11581 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
    11641 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
    11701 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
    11761 attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgagctctc
    11821 aagcataatc aggcacatca taagggtatc cggcgctgcc agcgtagtca ggcacatcat
    11881 aggggtagga tcctgtgccg gcgtaatcag gcacgtcata gggatacatg ccgctccctc
    11941 c
//
