LOCUS       sequence-455821-       11823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing peroxisome outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 11823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: PX4
FEATURES             Location/Qualifiers
     source          1..11823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..69)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(129..155)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(168..194)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(207..233)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(249..1019)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(join(1023..1733,1734..1736,1737..1739))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1023..1733,1734..1736,1737..1739))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1065..1086)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1414..1433
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(2060..2440)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2974..2991)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2974..2990)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2983..3005)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3193..3217
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4518..5147
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5576..6649
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6715..6909
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7145..7167)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7253..7393
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7308..7327
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7408..7425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7579..8167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7659..7678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8254..9048)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9473..9497
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9575..9749
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9790..10815)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10888..11233)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10925..10945)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11424..11445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11460..11465,11466..11483,11484..11490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11498..11514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11503..11525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11522..11538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11522..11538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(11555..11807)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ttacttatcg tcatcgtcct tgtaatcgat gtcgtgatcc ttatagtctc catcatggtc
       61 tttgtagtcc atggtaccgg atccactagt atttaaatgt cgaccccggg cccctgcaga
      121 agcttctaag cataatcagg cacatcataa gggtatccgg cgctgccagc gtagtcaggc
      181 acatcatagg ggtaggatcc tgtgccggcg taatcaggca cgtcataggg atacatgccg
      241 ctccctccct tttcggcaga ccgcagactg atttctccgc ccatccaggg tttgataact
      301 ccgtcctgtt ccagcagcag ggctccctgt ttatcaatcc cccggctaat cccgaaaatc
      361 tctttgtccc caatgatcag cttcacgggt ctattgatga agttatccag cttctcccac
      421 cgtgacagat atggagccag gccttcctgc tcgaacagtt ccagggctgc ccgcagctct
      481 cggatcagca tagcggccag agtattcctg tccaggttaa tccctgcttc ctgcagtgtg
      541 atccagccct gattgaccac gctttcctcc acgcgcctca tagccacgtt aatcccggcg
      601 ccaatgacga tctgtgcagc atcgcctgtt attccggcca gctccaccag gatgcctgcc
      661 agctttctat cctgcagata caggtcattg ggccatttga ctcgcacctt gtctgctccc
      721 agctttcgca gcgcttctgc catgacaatt ccgatgaccg ggcccaggcc gattgctgct
      781 ggtccccgct tcaggcgcca aaacatactc aggtacaggt tagctccaaa aggagagaac
      841 catttcctcc ctctgcttcc tctccctgcc tgctgatatt ctgcaatgca agcatcccca
      901 ctcttcagct cgccgattcg atccagcagg tactgattgg tggagtcgac cacaggcagg
      961 actgccacgc tcccgccgtc cagctgtccc agaatctgtt tagcgttcag cagcgggatc
     1021 atcttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     1081 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     1141 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     1201 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     1261 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     1321 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     1381 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     1441 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     1501 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     1561 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     1621 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     1681 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccata
     1741 ctagtctgag agagaagaat agcagtcaga acctgaagag acttcaatcc tcctcctttc
     1801 ttctttttcg tggttatctt tgcattttga tcagttgcag tcagcttttc caaagtctca
     1861 tcatcatcac tctgaccatt gcaaagacgc gaagaatcag gcctccggga agcgaccctt
     1921 gatttcaggt aaacagcagt tccaccagca gctacaatcc cagccgcaag cagtatagat
     1981 ttccgtcttg acgctacaag accccgaccc cgctcagtta actgcaatag ttgaagtgaa
     2041 ggcataagct ttctagagtc gatttggtgt atcgagattg gttatgaaat tcagatgcta
     2101 gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt atccatttct
     2161 gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc tgtaaagcgt
     2221 tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt caaaagtcgt
     2281 taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga aattcaggcc
     2341 cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa taaaaactga
     2401 ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctccctcg atcgagaaag
     2461 agatcaatgt tgagctgctt caaaagcaat gggattgacc agctcgcgga tcctacaggc
     2521 caaattcgct cttagccgta caatattact caccggtgcg atgcccccca tcgtaggtga
     2581 aggtggaaat taatgatcca tcttgagacc acaggcccac aacagctacc agtttcctca
     2641 agggtccacc aaaaacgtaa gcgcttacgt acatggtcga taagaaaagg caatttgtag
     2701 atgttaacat ccaacgtcgc tttcagggat cctacaggcc aaattcgctc ttagccgtac
     2761 aatattactc accggtgcga tgccccccat cgtaggtgaa ggtggaaatt aatgatccat
     2821 cttgagacca caggcccaca acagctacca gtttcctcaa gggtccacca aaaacgtaag
     2881 cgcttacgta catggtcgat aagaaaaggc aatttgtaga tgttaacatc caacgtcgct
     2941 ttcagggatc cgcgagctta tcgataccgt ggcactggcc gtcgttttac aacgtcgtga
     3001 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3061 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3121 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
     3181 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     3241 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     3301 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     3361 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     3421 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     3481 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     3541 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     3601 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     3661 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     3721 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     3781 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     3841 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     3901 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     3961 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     4021 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     4081 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     4141 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     4201 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     4261 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     4321 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     4381 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     4441 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     4501 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     4561 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     4621 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     4681 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     4741 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     4801 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4861 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     4921 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     4981 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     5041 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     5101 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     5161 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     5221 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     5281 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     5341 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     5401 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     5461 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     5521 gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     5581 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     5641 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     5701 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     5761 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     5821 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     5881 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     5941 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     6001 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     6061 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     6121 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     6181 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     6241 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     6301 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     6361 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     6421 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     6481 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     6541 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     6601 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     6661 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     6721 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     6781 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     6841 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     6901 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     6961 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     7021 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     7081 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     7141 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7201 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     7261 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     7321 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     7381 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7981 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8221 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     8281 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     8341 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     8401 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     8461 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     8521 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     8581 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     8641 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     8701 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     8761 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     8821 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     8881 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     8941 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     9001 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     9061 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     9121 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     9181 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     9241 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     9301 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     9361 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     9421 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     9481 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     9541 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
     9601 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     9661 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
     9721 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgacag atcccggtcg
     9781 gcatctactc tatttctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc
     9841 gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg
     9901 cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc
     9961 atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat
    10021 atacgcccgg agtcgtggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt
    10081 ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg
    10141 ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt
    10201 caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc
    10261 ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat
    10321 cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt
    10381 agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc
    10441 ggccgcagcg atcgcatcca tagcctccgc gaccggttgt agaacagcgg gcagttcggt
    10501 ttcaggcagg tcttgcaacg tgacaccctg tgaacggcgg gagatgcaat aggtcaggct
    10561 ctcgctaaac tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg
    10621 ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata
    10681 tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat
    10741 caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag
    10801 ttcaggcttt ttcatatctc attgcccccc ggatctgcga aagctcgaga gagatagatt
    10861 tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat
    10921 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
    10981 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
    11041 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
    11101 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
    11161 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
    11221 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
    11281 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct
    11341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11521 acaggaaaca gctatgacat gattacgaat tcccgatcta gtaacataga tgacaccgcg
    11581 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
    11641 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
    11701 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
    11761 acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg gaaattcgag
    11821 ctc
//
