LOCUS       sequence-455823-       12228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing peroxisome outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12228)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 12228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: PX1
FEATURES             Location/Qualifiers
     source          1..12228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..711,712..714,715..717))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1..711,712..714,715..717))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(43..64)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     392..411
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(718..1677)
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     promoter        complement(1998..2378)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(3131..3148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3131..3147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3140..3162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3350..3374
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4675..5304
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5733..6806
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6872..7066
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7302..7324)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7410..7550
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7465..7484
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7565..7582)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7736..8324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7816..7835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8411..9205)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9630..9654
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9732..9906
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9947..10972)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11045..11390)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(11082..11102)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11581..11602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11617..11622,11623..11640,11641..11647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11655..11671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11660..11682
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11679..11695
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11679..11695
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(11712..11964)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11984..12049)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(12109..12135)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(12148..12174)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(12187..12213)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
       61 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
      121 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      181 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      241 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      301 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      361 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      421 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      481 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
      541 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
      601 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
      661 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatctt
      721 ttcggcagac cgcagactga tttctccgcc catccagggt ttgataactc cgtcctgttc
      781 cagcagcagg gctccctgtt tatcaatccc ccggctaatc ccgaaaatct ctttgtcccc
      841 aatgatcagc ttcacgggtc tattgatgaa gttatccagc ttctcccacc gtggcagata
      901 tggagccagg ccttcctgct cgaacagttc cagggctgcc cgcagctctc ggatcagcgt
      961 agcggccaga gtattcctgt ccaggttaat ccctgcttcc tgcagtgtga tccagccctg
     1021 attgaccacg ctttcctcca cgcgcctcat agccacgtta atcccggcgc caatgacgat
     1081 ctgtgcagca tcgcctgtta ttccggccag ctccaccagg atgcctgcca gctttctatc
     1141 ctgcagatac aggtcattgg gccatttgac tcgcaccttg tctgctccca gctttcgcag
     1201 cgcttctgcc atgacaattc cgatgaccgg gcccaggccg attgctgctg gtccccgctt
     1261 caggcgccaa aacatactca ggtacaggtt agctccaaaa ggagagaacc atttcctccc
     1321 tctgcttcct ctccctgcct gctgatattc tgcaatgcaa gcatccccac tcttcagctc
     1381 gccgattcga tccagcaggt actgattggt ggagtcgacc acaggcagga ctgccacgct
     1441 cccgccgtcc agctgtccca gaatctgttt agcgttcagc agcgggatag gctcgggcag
     1501 agagtagccc tttccgggca cggtgaacac gtccacgccc cagtcgcgca gagtctgaat
     1561 gtgcttgttg atagcggccc tggacatgcc cagggtttct cccagctgtt cgccactatg
     1621 gaactcgcca ttagccagga gagcgatcag cttcagaggc acagtattgt ctttcatact
     1681 agtctgagag agaagaatag cagtcagaac ctgaagagac ttcaatcctc ctcctttctt
     1741 ctttttcgtg gttatctttg cattttgatc agttgcagtc agcttttcca aagtctcatc
     1801 atcatcactc tgaccattgc aaagacgcga agaatcaggc ctccgggaag cgacccttga
     1861 tttcaggtaa acagcagttc caccagcagc tacaatccca gccgcaagca gtatagattt
     1921 ccgtcttgac gctacaagac cccgaccccg ctcagttaac tgcaatagtt gaagtgaagg
     1981 cataagcttt ctagagtcga tttggtgtat cgagattggt tatgaaattc agatgctagt
     2041 gtaatgtatt ggtaatttgg gaagatataa taggaagcaa ggctatttat ccatttctga
     2101 aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc gttcttgctg taaagcgttg
     2161 tttggtacac ttttgactag cgaggcttgg cgtgtcagcg tatctattca aaagtcgtta
     2221 atggctgcgg atcaagaaaa agttggaata gaaacagaat acccgcgaaa ttcaggcccg
     2281 gttgccatgt cctacacgcc gaaataaacg accaaattag tagaaaaata aaaactgact
     2341 cggatactta cgtcacgtct tgcgcactga tttgaaaaat ctccctcgat cgagaaagag
     2401 atcaatgttg agctgcttca aaagcaatgg gattgaccag ctcgcggatc ctacaggcca
     2461 aattcgctct tagccgtaca atattactca ccggtgcgat gccccccatc gtaggtgaag
     2521 gtggaaatta atgatccatc ttgagaccac aggcccacaa cagctaccag tttcctcaag
     2581 ggtccaccaa aaacgtaagc gcttacgtac atggtcgata agaaaaggca atttgtagat
     2641 gttaacatcc aacgtcgctt tcagggatcc tacaggccaa attcgctctt agccgtacaa
     2701 tattactcac cggtgcgatg ccccccatcg taggtgaagg tggaaattaa tgatccatct
     2761 tgagaccaca ggcccacaac agctaccagt ttcctcaagg gtccaccaaa aacgtaagcg
     2821 cttacgtaca tggtcgataa gaaaaggcaa tttgtagatg ttaacatcca acgtcgcttt
     2881 cagggatcct acaggccaaa ttcgctctta gccgtacaat attactcacc ggtgcgatgc
     2941 cccccatcgt aggtgaaggt ggaaattaat gatccatctt gagaccacag gcccacaaca
     3001 gctaccagtt tcctcaaggg tccaccaaaa acgtaagcgc ttacgtacat ggtcgataag
     3061 aaaaggcaat ttgtagatgt taacatccaa cgtcgctttc agggatccgc gagcttatcg
     3121 ataccgtggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga
     3301 gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat
     3361 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
     3421 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
     3481 gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
     3541 cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg
     3601 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata
     3661 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg
     3721 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg
     3781 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg
     3841 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag
     3901 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg
     3961 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca
     4021 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca
     4081 cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc
     4141 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga
     4201 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca
     4261 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga
     4321 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc
     4381 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat
     4441 cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat
     4501 cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga
     4561 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc
     4621 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag
     4681 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta
     4741 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc
     4801 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac
     4861 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac
     4921 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg
     4981 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt
     5041 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg
     5101 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc
     5161 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca
     5221 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct
     5281 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa
     5341 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc
     5401 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca
     5461 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca
     5521 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct
     5581 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg
     5641 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat
     5701 ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg
     5761 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
     5821 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
     5881 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
     5941 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
     6001 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
     6061 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
     6121 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
     6181 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
     6241 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
     6301 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
     6361 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
     6421 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
     6481 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
     6541 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
     6601 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
     6661 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
     6721 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
     6781 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
     6841 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa
     6901 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
     6961 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
     7021 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
     7081 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
     7141 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
     7201 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
     7261 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7321 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7381 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     7441 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7501 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     7561 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7621 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7681 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7741 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7801 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7861 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7921 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8161 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8281 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8341 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     8401 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     8461 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     8521 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     8581 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     8641 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     8701 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     8761 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     8821 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     8881 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     8941 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     9001 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     9061 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     9121 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     9181 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     9241 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     9301 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     9361 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
     9421 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
     9481 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
     9541 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     9601 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     9661 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     9721 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
     9781 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     9841 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
     9901 gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactctat ttctttgccc
     9961 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
    10021 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
    10081 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
    10141 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc
    10201 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
    10261 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
    10321 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
    10381 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
    10441 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
    10501 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
    10561 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag
    10621 cctccgcgac cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
    10681 caccctgtga acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa
    10741 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
    10801 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
    10861 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
    10921 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt
    10981 gccccccgga tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc
    11041 agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat
    11101 agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg
    11161 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
    11221 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
    11281 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
    11341 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
    11401 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
    11461 tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11521 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11581 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    11641 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
    11701 tacgaattcc cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
    11761 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
    11821 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
    11881 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
    11941 ttattgccaa atgtttgaac gatcggggaa attcgagctc ttacttatcg tcatcgtcct
    12001 tgtaatcgat gtcgtgatcc ttatagtctc catcatggtc tttgtagtcc atggtaccgg
    12061 atccactagt atttaaatgt cgaccccggg cccctgcaga agcttctaag cataatcagg
    12121 cacatcataa gggtatccgg cgctgccagc gtagtcaggc acatcatagg ggtaggatcc
    12181 tgtgccggcg taatcaggca cgtcataggg atacatgccg ctccctcc
//
