LOCUS       sequence-45583-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Dmitriev PV, Vassetzky YS
  TITLE     A set of vectors for introduction of antibiotic resistance genes by 
            in vitro Cre-mediated recombination.
  JOURNAL   BMC Res Notes. 2008 Dec 23;1:135. doi: 10.1186/1756-0500-1-135.
  PUBMED    19105832
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2008-12-23"; volume: "1:135. doi"; pages: " 
            10.1186/1756-0500-1-135"
COMMENT     Sequence Label: pT-BS
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(95..128)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     755..774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
       61 accaagttta ctcatgtcga gggggggccc atggataact tcgtatagca tacattatac
      121 gaagttatgg cccggcctca ggattcttcc tttttcaata ttattgaagc atttatcagg
      181 gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
      241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      361 ctctttttcc gaaggtaact ggcttcagta gagcgcagat accaaatact gttcttctag
      421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggg
//
