LOCUS       sequence-455831-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of the red fluorescent 
            protein mCherry (TRE3G promoter) and constitutive expression of the 
            blue fluorescent protein tagBFP (PGK promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  TITLE     Morsut lab plasmids 2024
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_TRE3G_mCherry_WPRE_PGK_tagBFP
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..39
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(233..582)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(270..294)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    340..358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    376..394
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    412..430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    448..466
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    484..502
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    520..538
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    556..574
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    644..761
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cngnnnngnn gnggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
       61 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
      121 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
      181 ccatcccggg cccgcggtac cgcaagcttg atatcctgca gacgcgacgc gtaagtataa
      241 gacaaaagtg ttgtggaatt gctccaggcg atctgacggt tcactaaacg agctctgctt
      301 ttataggcgc ccaccgtaca cgcctacctc gacatacgtt ctctatcact gatagggagt
      361 aaactggata tacgttctct atcactgata gggagtaaac tgtagatacg ttctctatca
      421 ctgataggga gtaaactggt catacgttct ctatcactga tagggagtaa actccttata
      481 cgttctctat cactgatagg gagtaaagtc tgcatacgtt ctctatcact gatagggagt
      541 aaactcttca tacgttctct atcactgata gggagtaaac tcgagtgaag acgaaagggc
      601 ctcatcgata aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
      661 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      721 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
      781 tttggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
      841 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
      901 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
      961 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     1021 aatttgttat gttaaaccaa ttccncaa
//
