LOCUS       sequence-455832-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of the red fluorescent 
            protein mCherry (TRE3G promoter) and constitutive expression of the 
            blue fluorescent protein tagBFP (PGK promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  TITLE     Morsut lab plasmids 2024
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_TRE3G_mCherry_WPRE_PGK_tagBFP
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..26
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    44..62
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     180..204
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             290..994
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALX
                     GEIKQRLKLXDGGHYDAXVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDXLY"
     primer_bind     complement(435..453)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     703..722
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagttnctcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
       61 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
      121 atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga
      181 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatact
      241 tacgcgtcgc gtctgcagga tatcaagctt gcggtaccgc gggcccggga tggtgagcaa
      301 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
      361 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      421 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
      481 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
      541 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      601 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      661 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      721 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaangg
      781 cgagatcaag cagaggctga agctgaanga cggcggccac tacgacgctg angtcaagac
      841 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
      901 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
      961 ccgccactcc accggcggca tggacganct gtacagtaac ctgcnncatg caagcttgat
     1021 atcaagctta tcga
//
