LOCUS       sequence-455833-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of the tetracycline 
            transactivator tTA and of the blue fluorescent protein tagBFP, 
            separated by an internal ribosome entry site (SFFV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  TITLE     Morsut lab plasmids 2024
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_SFFV_tTA_IRES_tagBFP
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..14
                     /estimated_length=13
     CDS             join(126..743,744..749,750..785,786..788,789..824,825..827,
                     828..864,865..873)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDL*HAPRV"
ORIGIN
        1 gnnnnnnnnn nnnntcggcg cgccagtcct ccgacagact gagtcgcccg ggggggatct
       61 ggagctctcg agaattctca cgcgtcaagt ggagcaaggc aggtggacag tggatcggag
      121 ctaccatgtc tagactggac aagagcaaag tcataaactc tgctctggaa ttactcaatg
      181 aagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
      241 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg gcaatcgaga
      301 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
      361 tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc acatcgcgac ggggctaaag
      421 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
      481 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
      541 actttacact gggctgcgta ttggaggatc aggagcatca agtagcaaaa gaggaaagag
      601 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
      661 atcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
      721 aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt gacttagaca
      781 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
      841 acgattttga cctttgacat gctccccggg taataggatc cttgacttgc ggcccccctc
      901 tccctccccc cccctaacgt tactggccga agccgcttgg aatannncgg tgtgcgttgt
      961 ctatatgtnt tttcacatat tgcgtctttg gnaatgtgag ggcccngaaa cctggccctg
     1021 tcttntnacn agcattcctn gggtnnttcc cctc
//
