LOCUS       sequence-455834-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of the tetracycline 
            transactivator tTA and of the blue fluorescent protein tagBFP, 
            separated by an internal ribosome entry site (SFFV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  TITLE     Morsut lab plasmids 2024
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_SFFV_tTA_IRES_tagBFP
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..781)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 ancagcttgc atgcctgcag gtcgactcta gagtcgcggc ctcagtcagt cacgcatgtt
       61 gcaggtggga gttgcggcct taattaagct tgtgccccag tttgctaggg aggtcgcagt
      121 atctggccac tgccacctcg tgctgctcga cgtaggtctc gttgttggcc tccttgattc
      181 tttccagtct gtagtccaca tagtagacgc caggcatctt gaggttctta gcgggtttct
      241 tggatctata tgtggtcttg atgtttgcga tcagatggct cccgcccacg agcttcaggg
      301 ccatgtcgtt tctgccttcc aggccgccgt cagcggggta cagcgtctcg gtgaaggcct
      361 cccagccgag tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga
      421 tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca
      481 cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct
      541 tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc
      601 tagtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca
      661 tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt
      721 tgtccacggt gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctca
      781 tggacccgcc agcgctcatt tatcatcgtg tttttcaaag gaaaaccacg tcccngtggg
      841 ttcggggggc ctagacgttt ttttaacctc gactaaacac atgtaaagca tgtgcaccga
      901 ggccccagat canatcccat acaatggggt accttctggg catccttcag ccccttgttg
      961 atacgcttga ggagagccat ttgactcntt ccncaactat ccaactcaca cgtggnnnct
     1021 ggggtngtgc cgcctttgc
//
