LOCUS       sequence-455844-        2956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenoviral VA RNA I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
  TITLE     E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for
            AAV production in HEK293 cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 
            10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
  PUBMED    39670178
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-11-13"; volume: "32(4):101376. doi";
            pages: " 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12"
COMMENT     Sequence Label: JD73
FEATURES             Location/Qualifiers
     source          1..2956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(918..937)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(919..937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1029..1133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1134..1202,1203..1994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1352..1371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2192..2780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2681..2700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2934..2951
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggaggc tccatggtcg ggacgctctg gccggtgagg cgtgcgcagt
      361 cgttgacgct ctagaccgtg caaaaggaga gcctgtaagc gggcactctt ccgtggtctg
      421 gtggataaat tcgcaagggt atcatggcgg acgaccgggg ttcgaacccc ggatccggcc
      481 gtccgccgtg atccatgcgg ttaccgcccg cgtgtcgaac ccaggtgtgc gacgtcagac
      541 aacgggggag cgctcctttt ggcttccttc caggcgcggc ggctgctgcg ctagcttttt
      601 tggccactgg ccgcgcgcgg cgtaagcggt taggctggaa agcgaaagca ttaagtggct
      661 cgctccctgt agccggaggg ttattttcca agggttgagt cgcaggaccc ccggttcgag
      721 tctcgggccg gccggactgc ggcgaacggg ggtttgcctc cccgtcatgc aagaccccgc
      781 ttgcaaattc ctccggaaac agggacgagc cccttttttg cttttcccag atgcatccgg
      841 tgctgcggca gatgcgccat tcctgtagac ttcttaatta agacgtcaga attctcgagg
      901 cggccgcatg tgagtctccc tatagtgagt cgtattaatt tcgcgggcgg aacccctatt
      961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagtagcac caggcgttta
     1021 agggcacccg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1081 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1141 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1201 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1261 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1321 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1381 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1441 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1501 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1561 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1621 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1681 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1741 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1801 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga tctcgcggta
     1861 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1921 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcgtcactga
     1981 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2041 ttcattttta atttaaaagg atctaggtga agatccttta cgtgccgatc aaagtctcat
     2101 tttcgccaaa agttgtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2761 gggggcggag cctatggaaa aacgccagca atgcggcctt tttacggttc ctggcctttt
     2821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2881 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2941 cagtgagcga ggaagc
//
