LOCUS       sequence-455851-       13079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human stromal interaction 
            molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming 
            virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   Richard Eva, Evan Reid, Bart Nieuwenhuis Labs
  TITLE     Dual promoter expression vectors including endoplasmic reticulum 
            transgenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..13079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2423..2452
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4831..4851)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5249..5260)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6299..6318
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6492..6501
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6498..6500,6501..6503,6504..7217)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6543..6564)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6804..6823)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7151..7172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7312..7545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7570..7588)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7571..7588)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7878..7897)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8111..8130)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8230..8252
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8290..8308)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8376..8480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8481..8549,8550..9341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8699..8718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9512..10100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10001..10020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10254..10271
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10341..10361)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10346..10675
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10526..10661
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11809..11874
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12004..12024
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12449..12583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12486..12505)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12540..12559
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12751..13079,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggaacaaaa actcatctca gaagaggatc tgatgctggt
     2461 gctcgggctg ctggtagccg gagcggcgga cggatgcgag cttgtgcccc ggcacctccg
     2521 cgggcggcgg gcgactggct ctgccgcaac tgccgcctcc tctcccgccg cggcggccgg
     2581 cgatagcccg gcgctcatga cagatccctg catgtcactg agtccaccat gctttacaga
     2641 agaagacaga tttagtctgg aagctcttca aacaatacat aaacaaatgg atgatgacaa
     2701 agatggtgga attgaagtag aggaaagtga tgaattcatc agagaagata tgaaatataa
     2761 agatgctact aataaacaca gccatctgca cagagaagat aaacatataa cgattgagga
     2821 tttatggaaa cgatggaaaa catcagaagt tcataattgg acccttgaag acactcttca
     2881 gtggttgata gagtttgttg aactacccca atatgagaag aattttagag acaacaatgt
     2941 caaaggaacg acacttccca ggatagcagt gcacgaacct tcatttatga tctcccagtt
     3001 gaaaatcagt gaccggagtc acagacaaaa acttcagctc aaggcattgg atgtggtttt
     3061 gtttggacct ctaacacgcc cacctcataa ctggatgaaa gattttatcc tcacagtttc
     3121 tatagtaatt ggtgttggag gctgctggtt tgcttatacg cagaataaga catcaaaaga
     3181 acatgttgca aaaatgatga aagatttaga gagcttacaa actgcagagc aaagtctaat
     3241 ggacttacag gagaggcttg aaaaggcaca ggaagaaaac agaaatgttg ctgtagaaaa
     3301 gcaaaattta gagcgcaaaa tgatggatga aatcaattat gcaaaggagg aggcttgtcg
     3361 gctgagagag ctaagggagg gagctgaatg tgaattgagt agacgtcagt atgcagaaca
     3421 ggaattggaa caggttcgca tggctctgaa aaaggccgaa aaagaatttg aactgagaag
     3481 cagttggtct gttccagatg cacttcagaa atggcttcag ttaacacatg aagtagaagt
     3541 gcaatactac aatattaaaa gacaaaacgc tgaaatgcag ctagctattg ctaaagatga
     3601 ggttgctgct tcatatctga ttcaggcaga aaaaattaaa aagaagagaa gcacagtctt
     3661 tgggactctg cacgttgcac acagctcctc cctagatgag gtagaccaca aaattctgga
     3721 agcaaagaaa gctctctctg agttgacaac ttgtttacga gaacgacttt ttcgctggca
     3781 acaaattgag aagatctgtg gctttcagat agcccataac tcaggactcc ccagcctgac
     3841 ctcttccctt tattctgatc acagctgggt ggtgatgccc agagtctcca ttccacccta
     3901 tccaattgct ggaggagttg atgacttaga tgaagacaca cccccaatag tgtcacaatt
     3961 tcccgggacc atggctaaac ctcctggatc attagccaga agcagcagcc tgtgccgttc
     4021 acgccgcagc attgtgccgt cctcgcctca gcctcagcga gctcagcttg ctccacacgc
     4081 cccccacccg tcacaccctc ggcaccctca ccacccgcaa cacacaccac actccttgcc
     4141 ttcccctgat ccagatatcc tctcagtgtc aagttgccct gcgctttatc gaaatgaaga
     4201 ggaggaagag gccatttact tctctgctga aaagcaatgg gaagtgccag acacagcttc
     4261 agaatgtgac tccttaaatt cttccattgg aaggaaacag tctcctcctt taagcctcga
     4321 gatataccaa acattatctc cgcgaaagat atcaagagat gaggtgtccc tagaggattc
     4381 ctcccgaggg gattcgcctg taactgtgga tgtgtcttgg ggttctcccg actgtgtagg
     4441 tctgacagaa actaagagta tgatcttcag tcctgcaagc aaagtgtaca atggcatttt
     4501 ggagaaatcc tgtagcatga accagctttc cagtggcatc ccggtgccta aacctcgcca
     4561 cacatcatgt tcctcagctg gcaacgacag taaaccagtt caggaagccc caagtgttgc
     4621 cagaataagc agcatcccac atgacctttg tcataatgga gagaaaagca aaaagccatc
     4681 aaaaatcaaa agccttttta agaagaaatc taagtgaagc ggccgcgact ctagagtcga
     4741 cctgcaggca tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat
     4801 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4861 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4921 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4981 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5041 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5101 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5161 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5221 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5281 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc
     5341 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     5401 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     5461 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     5521 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     5581 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     5641 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     5701 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     5761 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg
     5821 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg
     5881 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg
     5941 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact
     6001 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt
     6061 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat
     6121 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag
     6181 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag
     6241 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg
     6301 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag
     6361 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa
     6421 ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg gtaccgagct
     6481 cggatcaatt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6541 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     6601 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6661 ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct
     6721 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6781 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6841 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6901 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg
     6961 ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg
     7021 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7081 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     7141 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7201 acgagctgta caagtaactc gagggaatta attcgagctc ggtaccttta agaccaatga
     7261 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7321 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat
     7561 tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
     7621 attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact
     7681 gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac
     7741 cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag
     7801 ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     7861 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     7921 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     7981 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     8041 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt
     8101 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     8161 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     8221 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     8281 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     8341 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8401 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8461 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8521 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8581 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8641 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8701 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8761 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8821 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8881 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8941 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9001 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9061 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9121 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9181 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9241 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9301 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9361 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9421 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9481 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9541 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9601 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9661 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9721 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9781 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9841 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9901 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9961 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10021 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10081 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10141 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10201 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10261 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    10321 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
    10381 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
    10441 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    10501 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    10561 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    10621 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    10681 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
    10741 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
    10801 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
    10861 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
    10921 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
    10981 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
    11041 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
    11101 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
    11161 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
    11221 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
    11281 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
    11341 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
    11401 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
    11461 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
    11521 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
    11581 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
    11641 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
    11701 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
    11761 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    11821 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    11881 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    11941 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    12001 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    12061 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    12121 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
    12181 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
    12241 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
    12301 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    12361 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    12421 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    12481 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12541 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    12601 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    12661 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    12721 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
    12781 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
    12841 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
    12901 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
    12961 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
    13021 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgc
//
