LOCUS       sequence-455854-       12701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-term transit peptide from Sapria himalayana Cytochrome C
            with demonstrated mito targeting; proof-of-concept for Plasmid 
            228371.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 12701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_CytC_87.18_SHI04112_TP_GFP_pK7fWG2_IVD_FP611
FEATURES             Location/Qualifiers
     source          1..12701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        294..473
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             507..1301
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(561..580)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1171..1190
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1341..1593
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1686..1710
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2231..3022
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3268..3856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3757..3776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4010..4027
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4042..4182
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4108..4127)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4268..4290
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4526..4720
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4786..5859)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6288..6917)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8248..8272
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        8942..9286
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    9298..9322
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10214..10238)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10240..10266
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        11169..11513
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    11613..11637
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11878..11902)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(11910..11912,11913..11915,11916..12629)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11910..11912,11913..11915,11916..12626)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11955..11976)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12216..12235)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12563..12584
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
       61 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
      121 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct
      181 ctagaggatc cccgggtacc gcgaattatc gatcatgagc ggagaattaa gggagtcacg
      241 ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc
      301 aacgttgaag gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt
      361 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
      421 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
      481 atattcactc tcaactcgat cgaggcatga ttgaacaaga tggattgcac gcaggttctc
      541 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
      601 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
      661 acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg tggctggcca
      721 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
      781 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
      841 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
      901 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
      961 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     1021 ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccac ggcgatgcct
     1081 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     1141 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     1201 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     1261 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgg
     1321 actctagcta gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
     1381 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     1441 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     1501 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     1561 tatcgcgcgc ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat
     1621 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     1681 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     1741 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     1801 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     1861 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     1921 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     1981 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     2041 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     2101 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     2161 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     2221 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     2281 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     2341 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     2401 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     2461 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     2521 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     2581 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     2641 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     2701 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     2761 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2821 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2881 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     2941 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     3001 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     3061 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     3121 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     3181 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3241 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3301 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3361 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     3421 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3481 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3541 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3601 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3661 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3721 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3781 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3841 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3901 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3961 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4021 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4081 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4141 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     4201 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4261 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     4321 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     4381 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     4441 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     4501 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     4561 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     4621 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     4681 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     4741 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     4801 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     4861 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     4921 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     4981 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     5041 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     5101 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     5161 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     5221 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     5281 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     5341 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     5401 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     5461 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     5521 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     5581 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
     5641 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
     5701 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
     5761 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
     5821 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
     5881 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
     5941 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     6001 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
     6061 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
     6121 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
     6181 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
     6241 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
     6301 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
     6361 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
     6421 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
     6481 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
     6541 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
     6601 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
     6661 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
     6721 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
     6781 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
     6841 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
     6901 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
     6961 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
     7021 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
     7081 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
     7141 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
     7201 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
     7261 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
     7321 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
     7381 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
     7441 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
     7501 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
     7561 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
     7621 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
     7681 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
     7741 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
     7801 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
     7861 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
     7921 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
     7981 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
     8041 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
     8101 actttaaagt actttaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg
     8161 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
     8221 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
     8281 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa gcttgagctc
     8341 tcccatatgg tcgactagag ccaagctgat ctcctttgcc ccggagatca ccatggacga
     8401 ctttctctat ctctacgatc taggaagaaa gttcgacgga gaaggtgacg ataccatgtt
     8461 caccaccgat aatgagaaga ttagcctctt caatttcaga aagaatgctg acccacagat
     8521 ggttagagag gctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata
     8581 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
     8641 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
     8701 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
     8761 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     8821 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc
     8881 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
     8941 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
     9001 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
     9061 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
     9121 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     9181 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     9241 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg agaattcaca
     9301 agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccatgcagag gtttttctcc
     9361 gccagatcga ttctcggtta cgccgtcaag acgcggagga ggtctttctc ttctcgttct
     9421 tcgtctctcc tattcgacga cactcagtta cagtttaaag aaagtgtatc caagtttgcg
     9481 caagatagga tcccgcacgg gatgaactca ctgatcaagg aaaatatgcg tatgatggtg
     9541 gtcatggaag gttcggtcaa cggctaccaa ttcaaatgca caggtgaagg agatggcaat
     9601 ccatacatgg gaactcaaac catgaggatc aaagtcgtcg agggaggacc tctgccattt
     9661 gcctttgaca ttcttgccac aagcttcatg tatggcagca agacttttat caagcacact
     9721 aaaggcattc ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt
     9781 acaagatacg aagatggtgg agtctttacc gttatgcagg acaccagcct tgaagatggc
     9841 tgtctcgttt accacgccaa agtcactggg gtaaactttc cctccaatgg tgccgtgatg
     9901 cagaagaaga ccaagggttg ggagccaaat acagagatgc tgtatccagc agatggtggt
     9961 ctgaggggat actctcaaat ggcactgaat gttgatggtg gtggctatct gtcttgctct
    10021 ttcgaaacaa cttacaggtc aaaaaagacc gtcgagaact tcaagatgcc cggtttccat
    10081 tttgttgatc accgcctgga aaggttagag gaaagtgaca aggaaatgtt cgtagtacaa
    10141 cacgaacacg cagttgccaa gttctgtgac cttccatcca aactgggacg tctttgaaag
    10201 ggtgggcgcg ccgacccagc tttcttgtac aaagtggttt acccatacga tgttccagat
    10261 tacgcttgag agctcgcata attctcaaca caacatatac aaaacaaacg aatctcaagc
    10321 aatcaagcat tctacttcta ttgcagcaat ttaaatcatt tcttttaaag caaaagcaat
    10381 tttctgaaaa ttttcaccat ttacgaacga tagccatggc actcatcttt ggcacagtca
    10441 acgctaacat cctgaaggaa gtgttcggtg gagctcggta cccggggatc ctctagagtc
    10501 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
    10561 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
    10621 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
    10681 ctaattccta aaaccaaaat ccagtgacct gcaggcatgc aagcttggca ctggccctac
    10741 gcggcaggtc tcatcaagac gatctacccg agtaataatc tccaggagat caaatacctt
    10801 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
    10861 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
    10921 cataaaccaa ggcaagtaat agagattgga gtctctaaga aagtagttcc tactgaatca
    10981 aaggccatgg agtcaaaaat tcagatcgag gatctaacag aactcgccgt gaagactggc
    11041 gaacagttca tacagagtct tttacgactc aatgacaaga agaaaatctt cgtcaacatg
    11101 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa
    11161 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
    11221 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc
    11281 catcattgcg ataaaggaaa ggctatcgtt caagatgccc ctgccgacag tggtcccaaa
    11341 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
    11401 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
    11461 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gactccggta
    11521 tttttacaac aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta
    11581 gtcgacctgc aggcggccgc actagtgata tcacaagttt gtacaaaaaa gcaggctccg
    11641 cggccgcccc cttcaccatg cttaaggctg ctggaagaag attttctctt attgcttgga
    11701 gaggatcttc tatttcttct tttacttctg cttctcattg ttatattcat tctattcatg
    11761 gaaatgctgc tgctgctgga gttcatagac cagatactct ttctattgct ggaggactta
    11821 ttagaggatt ttctgctgga gctcttgata gatctcatga aaagggtggg cgcgccgacc
    11881 cagctttctt gtacaaagtg gtgatatcaa tggtgagcaa gggcgaggag ctgttcaccg
    11941 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
    12001 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
    12061 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
    12121 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
    12181 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
    12241 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
    12301 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
    12361 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
    12421 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
    12481 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
    12541 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
    12601 ctctcggcat ggacgagctg tacaagtaac cgcggccatg ctagagtccg caaaaatcac
    12661 cagtctctct ctacaaatct atctctctct atttttctcc a
//
