LOCUS       sequence-455867-        7110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei.
  JOURNAL   Gene. 2009 Feb 1. 430(1-2):123-31.
  PUBMED    19010402
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2009 
            Feb 1. 430(1-2):123-31."
COMMENT     Sequence Label: pMo168
FEATURES             Location/Qualifiers
     source          1..7110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..772
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             773..1435
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     promoter        join(1906..1911,1912..1927,1928..1934)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     2977..2999
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        3123..3226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3227..4021
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3281..3300)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3891..3910
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5530..5549
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5616..5718)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg
       61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt
      121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt
      181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac
      241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc
      301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga agggcgaagc ccgcccgcct
      361 gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc caccttgggc
      421 aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt tgccggaggg
      481 ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga
      541 actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa aggggggtac
      601 gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa gaaaatctgt
      661 aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa agttgcagct
      721 gattgcgcat ggtgccgcaa ccgtgcggca cccctaccgc atggagataa gcatggccac
      781 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
      841 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
      901 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
      961 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     1021 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     1081 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     1141 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     1201 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     1261 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     1321 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     1381 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     1441 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     1501 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     1561 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     1621 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     1681 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     1741 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     1801 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     1861 atatcagggc cccgctagcc agatcttccc gggaagcttg agctgttgac cattaatcat
     1921 cggctcgtat aatgtgtgga atatcacaga aggagacaga atactatgaa caaaggtgta
     1981 atgcgaccgg gccatgtgca gctgcgtgta ctggacatga gcaaggccct ggaacactac
     2041 gtcgagttgc tgggcctgat cgagatggac cgtgacgacc agggccgtgt ctatctgaag
     2101 gcttggaccg aagtggataa gttttccctg gtgctacgcg aggctgacga gccgggcatg
     2161 gattttatgg gtttcaaggt tgtggatgag gatgctctcc ggcaactgga gcgggatctg
     2221 atggcatatg gctgtgccgt tgagcagcta cccgcaggtg aactgaacag ttgtggccgg
     2281 cgcgtgcgct tccaggcccc ctccgggcat cacttcgagt tgtatgcaga caaggaatat
     2341 actggaaagt ggggtttgaa tgacgtcaat cccgaggcat ggccgcgcga tctgaaaggt
     2401 atggcggctg tgcgtttcga ccacgccctc atgtatggcg acgaattgcc ggcgacctat
     2461 gacctgttca ccaaggtgct cggtttctat ctggccgaac aggtgctgga cgaaaatggc
     2521 acgcgcgtcg cccagtttct cagtctgtcg accaaggccc acgacgtggc cttcattcac
     2581 catccggaaa aaggccgcct ccatcatgtg tccttccacc tcgaaacctg ggaagacttg
     2641 cttcgcgccg ccgacctgat ctccatgacc gacacatcta tcgatatcgg cccaacccgc
     2701 cacggcctca ctcacggcaa gaccatctac ttcttcgacc cgtccggtaa ccgcaacgaa
     2761 gtgttctgcg ggggagatta caactacccg gaccacaaac cggtgacctg gaccaccgac
     2821 cagctgggca aggcgatctt ttaccacgac cgcattctca acgaacgatt catgaccgtg
     2881 ctgacctgag cggccgccct gcagcggatc cctctagatg catgcgacgg gcttgtctgc
     2941 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3001 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3061 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3181 gacaataacc ctgataaatg cttcaataat ttgaaaaagg aagagtatga ttgaacaaga
     3241 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3301 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     3361 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc
     3421 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     3481 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     3541 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     3601 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     3661 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     3721 cgcgccagcc gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt
     3781 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     3841 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     3901 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     3961 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4021 attaattaaa ctagtgcttg aattccgcaa ttaatgtaag ttagctcact cattaggcac
     4081 cgggatctcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     4141 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     4201 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     4261 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     4321 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc
     4381 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
     4441 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
     4501 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
     4561 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
     4621 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
     4681 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
     4741 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
     4801 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
     4861 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
     4921 aactcaacga gctggacgcg gatgaacagg cagacatctg ccgcggcata ccaggcctga
     4981 atcgccccat catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag
     5041 gtggaccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga
     5101 agatgcgtga tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt
     5161 cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga
     5221 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     5281 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     5341 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     5401 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     5461 ccggtgagaa tggcaaaagc taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     5521 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     5581 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     5641 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     5701 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     5761 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     5821 tcgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5881 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcgcccgcg ttcctgctgg
     5941 cgctgggcct gtttctggcg ctggacttcc cgctgttccg tcagcagctt ttcgcccacg
     6001 gccttgatga tcgcggcggc cttggcctgc atatcccgat tcaacggccc cagggcgtcc
     6061 agaacgggct tcaggcgctc ccgaaggtct cgggccgtct cttgggcttg atcggccttc
     6121 ttgcgcatct cacgcgctcc tgcggcggcc tgtagggcag gctcataccc ctgccgaacc
     6181 gcttttgtca gccggtcggc cacggcttcc ggcgtctcaa cgcgctttga gattcccagc
     6241 ttttcggcca atccctgcgg tgcataggcg cgtggctcga ccgcttgcgg gctgatggtg
     6301 acgtggccca ctggtggccg ctccagggcc tcgtagaacg cctgaatgcg cgtgtgacgt
     6361 gccttgctgc cctcgatgcc ccgttgcagc cctagatcgg ccacagcggc cgcaaacgtg
     6421 gtctggtcgc gggtcatctg cgctttgttg ccgatgaact ccttggccga cagcctgccg
     6481 tcctgcgtca gcggcaccac gaacgcggtc atgtgcgggc tggtttcgtc acggtggatg
     6541 ctggccgtca cgatgcgatc cgccccgtac ttgtccgcca gccacttgtg cgccttctcg
     6601 aagaacgccg cctgctgttc ttggctggcc gacttccacc attccgggct ggccgtcatg
     6661 acgtactcga ccgccaacac agcgtccttg cgccgcttct ctggcagcaa ctcgcgcagt
     6721 cggcccatcg cttcatcggt gctgctggcc gcccagtgct cgttctctgg cgtcctgctg
     6781 gcgtcagcgt tgggcgtctc gcgctcgcgg taggcgtgct tgagactggc cgccacgttg
     6841 cccattttcg ccagcttctt gcatcgcatg atcgcgtatg ccgccatgcc tgcccctccc
     6901 ttttggtgtc caaccggctc gacgggggca gcgcaaggcg gtgcctccgg cgggccactc
     6961 aatgcttgag tatactcact agactttgct tcgcaaagtc gtgaccgcct acggcggctg
     7021 cggcgcccta cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc
     7081 agcccgtgga tatgtggacg atggccgcga
//
