LOCUS       sequence-45587-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA
  TITLE     Differential WNT activity in colorectal cancer confers limited 
            tumorigenic potential and is regulated by MAPK signaling.
  JOURNAL   Cancer Res. 2012 Feb 8.
  PUBMED    22318865
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2012 Feb 8."
COMMENT     Sequence Label: FOP-GFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(70..101)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    306..423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(690..734)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tggtggcgac cggtggatcc aacagtaccg gaatgccaag ctttttacca acagtaccgg
       61 aatgccaagc tggaattcga gcttccatta tataccctct agagtctaga tctcgagccc
      121 gggctagcac gcgggcgcgc ccctttggcc ttaccccctt tggccttacc ccctttggcc
      181 ttaccccctt tggcccttag acctttggcc ttaccccctt tggccttacc ccctttggcc
      241 tcgcgtaaga gctcggtacc acgcgtcgtg atatcaagct tctcgaggct agtctcgtga
      301 tcgataaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
      361 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
      421 aaagttaacc gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc
      481 tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg
      541 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca
      601 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac
      661 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat
      721 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt
      781 gtgtaattgt taatttctct
//
