LOCUS       sequence-455870-       12710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-term transit peptide from Sapria himalayana Methionine 
            aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 440.10; 
            determination of S. himalayana MetRS target localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, 
            Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
  TITLE     Photosynthetic demands on translational machinery drive retention of
            redundant tRNA metabolism in plant organelles.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 
            10.1073/pnas.2421485121. Epub 2024 Dec 18.
  PUBMED    39693336
REFERENCE   2  (bases 1 to 12710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-12-24"; volume: "121(52):e2421485121. 
            doi"; pages: " 10.1073/pnas.2421485121. Epub 2024 Dec 18"
COMMENT     Sequence Label: Shim_MetRS_440.109_TP_GFP_pK7fWG2_IVD_FP611
FEATURES             Location/Qualifiers
     source          1..12710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4981..5005
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        5675..6019
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    6031..6055
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6947..6971)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6973..6999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        7902..8246
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    8346..8370
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8620..8644)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(8652..8654,8655..8657,8658..9371)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8652..8654,8655..8657,8658..9368)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8697..8718)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8958..8977)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9305..9326
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        9737..9916
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9950..10744
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10004..10023)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10614..10633
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      10784..11036
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11129..11153
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11674..12465
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa agtactttaa
     4861 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
     4921 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     4981 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     5041 aatctgatcc aagctcaagc taagcttgag ctctcccata tggtcgacta gagccaagct
     5101 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
     5161 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
     5221 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggctgcag gtcaacatgg
     5281 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     5341 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     5401 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     5461 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     5521 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     5581 agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata
     5641 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
     5701 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
     5761 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
     5821 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     5881 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     5941 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     6001 catttcattt ggagaggacc tcgagaattc acaagtttgt acaaaaaagc aggctccgcg
     6061 gccgccccct tcaccatgca gaggtttttc tccgccagat cgattctcgg ttacgccgtc
     6121 aagacgcgga ggaggtcttt ctcttctcgt tcttcgtctc tcctattcga cgacactcag
     6181 ttacagttta aagaaagtgt atccaagttt gcgcaagata ggatcccgca cgggatgaac
     6241 tcactgatca aggaaaatat gcgtatgatg gtggtcatgg aaggttcggt caacggctac
     6301 caattcaaat gcacaggtga aggagatggc aatccataca tgggaactca aaccatgagg
     6361 atcaaagtcg tcgagggagg acctctgcca tttgcctttg acattcttgc cacaagcttc
     6421 atgtatggca gcaagacttt tatcaagcac actaaaggca ttcctgattt ctttaaacag
     6481 tcctttcctg agggttttac ttgggaaaga gttacaagat acgaagatgg tggagtcttt
     6541 accgttatgc aggacaccag ccttgaagat ggctgtctcg tttaccacgc caaagtcact
     6601 ggggtaaact ttccctccaa tggtgccgtg atgcagaaga agaccaaggg ttgggagcca
     6661 aatacagaga tgctgtatcc agcagatggt ggtctgaggg gatactctca aatggcactg
     6721 aatgttgatg gtggtggcta tctgtcttgc tctttcgaaa caacttacag gtcaaaaaag
     6781 accgtcgaga acttcaagat gcccggtttc cattttgttg atcaccgcct ggaaaggtta
     6841 gaggaaagtg acaaggaaat gttcgtagta caacacgaac acgcagttgc caagttctgt
     6901 gaccttccat ccaaactggg acgtctttga aagggtgggc gcgccgaccc agctttcttg
     6961 tacaaagtgg tttacccata cgatgttcca gattacgctt gagagctcgc ataattctca
     7021 acacaacata tacaaaacaa acgaatctca agcaatcaag cattctactt ctattgcagc
     7081 aatttaaatc atttctttta aagcaaaagc aattttctga aaattttcac catttacgaa
     7141 cgatagccat ggcactcatc tttggcacag tcaacgctaa catcctgaag gaagtgttcg
     7201 gtggagctcg gtacccgggg atcctctaga gtccgcaaaa atcaccagtc tctctctaca
     7261 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
     7321 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     7381 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
     7441 cctgcaggca tgcaagcttg gcactggccc tacgcggcag gtctcatcaa gacgatctac
     7501 ccgagtaata atctccagga gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa
     7561 agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt
     7621 actattccag tatggacgat tcaaggcttg cttcataaac caaggcaagt aatagagatt
     7681 ggagtctcta agaaagtagt tcctactgaa tcaaaggcca tggagtcaaa aattcagatc
     7741 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     7801 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct cgtctactcc
     7861 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     7921 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
     7981 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
     8041 gttcaagatg cccctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     8101 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
     8161 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     8221 ggaagttcat ttcatttgga gaggactccg gtatttttac aacaattacc acaacaaaac
     8281 aaacaacaaa caacattaca atttactatt ctagtcgacc tgcaggcggc cgcactagtg
     8341 atatcacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgagaggag
     8401 ttggatttta ttgtgttgga gctcataaga gagctgctat tgatggagct tattctgctg
     8461 ctgcttatct tccaaagtct cttcttattc aaagacatgg aaagcatgct aagactacta
     8521 tgggacatgt tccactttct aataatgcta ctgctcaatg ttggactgaa gctaagattc
     8581 caattcttgg aaagagaaat attaagggtg ggcgcgccga cccagctttc ttgtacaaag
     8641 tggtgatatc aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     8701 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     8761 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     8821 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     8881 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     8941 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     9001 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     9061 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     9121 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     9181 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     9241 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     9301 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     9361 tgtacaagta accgcggcca tgctagagtc cgcaaaaatc accagtctct ctctacaaat
     9421 ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag
     9481 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     9541 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacct
     9601 gcaggcatgc gacgtcgggc cctctagagg atccccgggt accgcgaatt atcgatcatg
     9661 agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt
     9721 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tgagccgcgg gtttctggag
     9781 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg
     9841 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc
     9901 cctcggtatc caattagagt ctcatattca ctctcaactc gatcgaggca tgattgaaca
     9961 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    10021 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    10081 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc
    10141 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    10201 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    10261 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    10321 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    10381 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    10441 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct
    10501 cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    10561 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    10621 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    10681 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    10741 ctgagcggga ctctggggtt cggactctag ctagagtcaa gcagatcgtt caaacatttg
    10801 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
    10861 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
    10921 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
    10981 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacc
    11041 ggcatgcaag ctgataattc aattcggcgt taattcagta cattaaaaac gtccgcaatg
    11101 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
    11161 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
    11221 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
    11281 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
    11341 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca
    11401 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
    11461 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
    11521 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
    11581 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
    11641 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
    11701 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
    11761 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
    11821 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
    11881 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
    11941 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
    12001 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
    12061 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
    12121 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
    12181 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
    12241 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
    12301 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
    12361 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
    12421 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
    12481 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
    12541 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
    12601 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag
    12661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
