LOCUS       sequence-45588-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA
  TITLE     Differential WNT activity in colorectal cancer confers limited 
            tumorigenic potential and is regulated by MAPK signaling.
  JOURNAL   Cancer Res. 2012 Feb 8.
  PUBMED    22318865
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2012 Feb 8."
COMMENT     Sequence Label: pUG2K
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..67
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(74..106,107..109,110..640)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             656..709
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gggatctgga gcaacaaact tctcactact caaacaagca ggtgacgtgg aggagaatcc
       61 cgggccttct agaatgactg aatataaact tgtggtagtt ggagctgttg gcgtaggcaa
      121 gagtgccttg acgatacagc taattcagaa tcattttgtg gacgaatatg atccaacaat
      181 agaggattcc tacaggaagc aagtagtaat tgatggagaa acctgtctct tggatattct
      241 cgacacagca ggtcaagagg agtacagtgc aatgagggac cagtacatga ggactgggga
      301 gggctttctt tgtgtatttg ccataaataa tactaaatca tttgaagata ttcaccatta
      361 tagagaacaa attaaaagag ttaaggactc tgaagatgta cctatggtcc tagtaggaaa
      421 taaatgtgat ttgccttcca gaacagtaga cacaaaacag gctcaggact tagcaagaag
      481 ttatggaatt ccttttattg aaacatcagc aaagacaaga cagggtgttg atgatgcctt
      541 ctatacatta gttcgagaaa ttcgaaaaca taaagaaaag atgagcaaag atggtaaaaa
      601 gaagaaaaag aagtcaaaga caaagtgtgt aattatgtaa ggatccggct ccggcgaggg
      661 caggggaagt ctactaacat gcggggacgt ggaggaaaat cccggcccag ctagcgtgtg
      721 atcagaattc gttccggagt cgtcgactcg acaatcaacc tctggattac aaaatttgtg
      781 aaagattgac tggtattctt aacta
//
