LOCUS       sequence-45589-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA
  TITLE     Differential WNT activity in colorectal cancer confers limited 
            tumorigenic potential and is regulated by MAPK signaling.
  JOURNAL   Cancer Res. 2012 Feb 8.
  PUBMED    22318865
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2012 Feb 8."
COMMENT     Sequence Label: FOP-GFP.mC
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(528..572)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tggtggcgac cggtggatca tcccatggtg gctttaccaa caggtaccgg aatgccaagc
       61 ttacttagat cgcagatctc gagtaccgga tccattatat accctctagc ctttggcctt
      121 accccctttg gccttacccc ctttggcctt accccctttg gcctagacct ttggccttac
      181 cccctttggc cttaccccct ttggccctgc aggcatgcaa gcttcagctg ctcgagcccg
      241 ggctatcgag gctagtctcg tgataaccga taccgtcgag atccgttcac taatcgaatg
      301 gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc
      361 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
      421 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
      481 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
      541 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
      601 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
      661 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
      721 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
      781 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
      841 agatgct
//
