LOCUS       sequence-455911-        9715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Leng K, Rose IVL, Kim H, Xia W, Romero-Fernandez W, Rooney B, Koontz
            M, Li E, Ao Y, Wang S, Krawczyk M, Tcw J, Goate A, Zhang Y, Ullian 
            EM, Sofroniew MV, Fancy SPJ, Schrag MS, Lippmann ES, Kampmann M
  TITLE     CRISPRi screens in human iPSC-derived astrocytes elucidate 
            regulators of distinct inflammatory reactive states.
  JOURNAL   Nat Neurosci. 2022 Nov;25(11):1528-1542. doi: 
            10.1038/s41593-022-01180-9. Epub 2022 Oct 27.
  PUBMED    36303069
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-022-01180-9"; journalName: "Nat Neurosci"; date: 
            "2022-11"; volume: "25"; issue: "11"; pages: "1528-1542"
COMMENT     Sequence Label: pKL100
FEATURES             Location/Qualifiers
     source          1..9715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(54..122,123..914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(272..291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1085..1673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1574..1593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1721..2557
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    2590..2608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2626..2644
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2662..2680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2698..2716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2734..2752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2770..2788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2806..2824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2869..2893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4512..4565
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     enhancer        5970..6349
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6355..6579
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6568..6585
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    6601..6634
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6910..6929)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7179..7197
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7339..7348
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             7386..7654
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(7667..7878)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    7914..8138
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8159..8758)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8242..8262)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8739..8758
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(8768..8821)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    8845..8870
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    8877..8910
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8912..9715
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aagggcgaat tcaaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
       61 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1201 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1681 gcaacgcggc ctttttacgg ttcctggcga attcgccctt agagcagagc caggaacccc
     1741 tgtagggaag gggcaggaga gccaggggca tgagatggtg gacgaggaag ggggacaggg
     1801 aagcctgagc gcctctcctg ggcttgccaa ggactcaaac ccagaagccc agagcagggc
     1861 cttagggaag cgggaccctg ctctgggcgg aggaatatgt cccagatagc actggggact
     1921 ctttaaggaa agaaggatgg agaaagagaa agggagtaga ggcggccacg acctggtgaa
     1981 cacctaggac gcaccattct cacaaaggga gttttccaca cggacacccc cctcctcacc
     2041 acagccctgc caggacgggg ctggctactg gccttatctc acaggtaaaa ctgacgcacg
     2101 gaggaacaat ataaattggg gactagaaag gtgaagagcc aaagttagaa ctcaggacca
     2161 acttattctg attttgtttt tccaaactgc ttctcctctt gggaagtgta aggaagctgc
     2221 agcaccagga tcagtgaaac gcaccagaca gccgcgtcag agcagctcag gttctgggag
     2281 agggtagcgc agggtggcca ctgagaaccg ggcaggtcac gcatcccccc cttccctccc
     2341 accccctgcc aagctctccc tcccaggatc ctctctggct ccatcgtaag caaaccttag
     2401 aggttctggc aaggagagag atggctccag gaaatggggg tgtgtcacca gataaggaat
     2461 ctgcctaaca ggaggtgggg gttagaccca atatcaggag actaggaagg aggaggccta
     2521 aggatggggc ttttctgtca ccaatcctgt ccctagtaaa gctgatatct ccaccccaca
     2581 gtggtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2641 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2701 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     2761 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     2821 gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt
     2881 gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca actttccgta
     2941 ccacttccta ccctcgtaaa cttaaggccg ccaccatgta ttctccgctc tgtctcaccc
     3001 aggatgaatt tcatcctttc atcgaagcac ttctgcccca cgtccgagcc tttgcctaca
     3061 catggttcaa cctgcaggcc cgaaaacgaa aatacttcaa aaaacatgaa aagcgtatgt
     3121 caaaagaaga agagagagcc gtgaaggatg aattgctaag tgaaaaacca gaggtcaagc
     3181 agaagtgggc atctcgactt ctggcaaagt tgcggaaaga tatccgaccc gaatatcgag
     3241 aggattttgt tcttacagtt acagggaaaa aacctccatg ttgtgttctt tccaacccag
     3301 accagaaagg caagatgcga agaattgact gcctccgcca ggcagataaa gtctggaggt
     3361 tggaccttgt tatggtgatt ttgtttaaag gtattccgct ggaaagtact gatggcgagc
     3421 gccttgtaaa gtccccacaa tgctctaatc cagggctctg tgtccaaccc catcacatag
     3481 gggtttctgt taaggaactc gatttatatt tggcatactt tgtgcatgca gcagattcaa
     3541 gtcaatctga aagtcccagc cagccaagtg acgctgacat taaggaccag ccagaaaatg
     3601 gacatttggg cttccaggac agttttgtca catcaggtgt ttttagtgtc actgagctag
     3661 taagagtgtc acagacacca atagctgcag gaactggccc aaatttttct ctctcagatt
     3721 tggaaagttc ttcatactac agcatgagtc caggagcaat gaggaggtct ttacccagca
     3781 catcctctac gagctccaca aagcgcctca agtctgtgga ggatgaaatg gacagtcctg
     3841 gtgaggagcc attttataca ggccaagggc gctccccagg aagtggcagt cagtcaagtg
     3901 gatggcatga agtggagcca ggaatgccat ctccaaccac actgaagaag tcggagaagt
     3961 ctggtttcag cagcccctcc ccttcacaga cctcctccct gggaacggcg ttcacacagc
     4021 atcaccgacc tgtcattaca ggacccagag caagtccgca tgcaacacca tcgactcttc
     4081 atttcccgac atcacccatt atccagcagc ctgggcctta cttctcacac ccagccatcc
     4141 gctatcaccc tcaggagacg ctgaaagaat ttgtccaact tgtctgccct gatgctggtc
     4201 agcaggctgg acaggtgggg ttcctcaatc ccaatgggag cagccaaggc aaggtgcaca
     4261 acccattcct tcccacccca atgttgccac cgccaccgcc accaccgatg gccaggcctg
     4321 tgcctctgcc ggtgccagac acaaagcctc caaccacgtc aacagaagga ggtgcagcct
     4381 cccccacgtc accaacctac tcgacaccca gcacctcccc cgcaaaccga ttcgtcagtg
     4441 ttggaccacg ggatccaagc tttgtaaata tccctcaaca gacacagtcc tggtacctgg
     4501 gaggtagcgg agagggcaga ggcagtctgc tgacatgcgg tgacgtggaa gagaatcccg
     4561 gccctatgaa tctcctggac cccttcatga agatgaccga cgagcaggag aagggcctgt
     4621 ccggcgcccc cagccccacc atgtccgagg actccgcggg ctcgccctgc ccgtcgggct
     4681 ccggctcgga caccgagaac acgcggcccc aggagaacac gttccccaag ggcgagcccg
     4741 atctgaagaa ggagagcgag gaggacaagt tccccgtgtg catccgcgag gcggtcagcc
     4801 aggtgctcaa aggctacgac tggacgctgg tgcccatgcc ggtgcgcgtc aacggctcca
     4861 gcaagaacaa gccgcacgtc aagcggccca tgaacgcctt catggtgtgg gcgcaggcgg
     4921 cgcgcaggaa gctcgcggac cagtacccgc acttgcacaa cgccgagctc agcaagacgc
     4981 tgggcaagct ctggagactt ctgaacgaga gcgagaagcg gcccttcgtg gaggaggcgg
     5041 agcggctgcg cgtgcagcac aagaaggacc acccggatta caagtaccag ccgcggcgga
     5101 ggaagtcggt gaagaacggg caggcggagg cagaggaggc cacggagcag acgcacatct
     5161 cccccaacgc catcttcaag gcgctgcagg ccgactcgcc acactcctcc tccggcatga
     5221 gcgaggtgca ctcccccggc gagcactcgg ggcaatccca gggcccaccg accccaccca
     5281 ccacccccaa aaccgacgtg cagccgggca aggctgacct gaagcgagag gggcgcccct
     5341 tgccagaggg gggcagacag ccccctatcg acttccgcga cgtggacatc ggcgagctga
     5401 gcagcgacgt catctccaac atcgagacct tcgatgtcaa cgagtttgac cagtacctgc
     5461 cgcccaacgg ccacccgggg gtgccggcca cgcacggcca ggtcacctac acgggcagct
     5521 acggcatcag cagcaccgcg gccaccccgg cgagcgcggg ccacgtgtgg atgtccaagc
     5581 agcaggcgcc gccgccaccc ccgcagcagc ccccacaggc cccgccggcc ccgcaggcgc
     5641 ccccgcagcc gcaggcggcg cccccacagc agccggcggc acccccgcag cagccacagg
     5701 cgcacacgct gaccacgctg agcagcgagc cgggccagtc ccagcgaacg cacatcaaga
     5761 cggagcagct gagccccagc cactacagcg agcagcagca gcactcgccc caacagatcg
     5821 cctacagccc cttcaacctc ccacactaca gccccccccc ccgcccccat cgctgcacaa
     5881 aataattaaa aaataaataa atacaaaatt gggggtgggg agggggggga gatggggaga
     5941 gtgaagcaga acgtggggct cacctcgacc atggtaatag cgatgactaa tacgtagatg
     6001 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     6061 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     6121 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     6181 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     6241 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     6301 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg agacccatag
     6361 agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct
     6421 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
     6481 cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg
     6541 aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgggttt
     6601 ataacttcgt ataatgtatg ctatacgaag ttatggtacc ttactcgtcc atgccgccgg
     6661 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
     6721 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
     6781 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
     6841 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
     6901 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
     6961 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
     7021 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
     7081 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
     7141 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
     7201 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
     7261 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
     7321 cctcctcgcc cttgctcacc atggtggcga ccggtagcgc tagcgtaggc gccggtcaca
     7381 gcttggatct gtaacggcgc agaacagaaa acgaaacaaa gacgtagagt tgagcaagca
     7441 gggtcaggca aagcgtggag agccggctga gtctaggtag gctccaaggg agcgccggac
     7501 aaaggcccgg tctcgacctg agctttaaac ttacctagac ggcggacgca gttcaggagg
     7561 caccacaggc gggaggcggc agaacgcgac tcaaccggcg tggatggcgg cctcaggtag
     7621 ggcggcgggc gcgtgaagga gagatgcgag cccctcgaag cttcagctgt gttctggcgg
     7681 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
     7741 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
     7801 accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg
     7861 gcgatgtgcg ctctgcccac tgacgggcac cggagcgatc gcagatcatg catccataga
     7921 gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg
     7981 ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc
     8041 attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga
     8101 ggggcaaaca acagatggct ggcaactaga aggcacaggt ttaaacgggc cctctagatc
     8161 aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg
     8221 cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca
     8281 ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc
     8341 ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct
     8401 ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac
     8461 cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct
     8521 ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt
     8581 cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt
     8641 ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta
     8701 cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatct
     8761 cgagcctagg gccgggattc tcctccacgt caccgcatgt tagaagactt cctctgccct
     8821 ctccgctgcc agatctctcg aggccctgtg ggaggaagag aagaggtcag aagcttataa
     8881 cttcgtataa tgtatgctat acgaagttat tgccccactg tggggtggag gggacagata
     8941 aaagtaccca gaaccagagc cacattaacc ggccctggga atataaggtg gtcccagctc
     9001 ggggacacag gatccctgga ggcagcaaac atgctgtcct gaagtggaca taggggcccg
     9061 ggttggagga agaagactag ctgagctctc ggacccctgg aagatgccat gacagggggc
     9121 tggaagagct agcacagact agagaggtaa ggggggtagg ggagctgccc aaatgaaagg
     9181 agtgagaggt gacccgaatc cacaggagaa cggggtgtcc aggcaaagaa agcaagagga
     9241 tggagaggtg gctaaagcca gggagacggg gtactttggg gttgtccaga aaaacggtga
     9301 tgatgcaggc ctacaagaag gggaggcggg acgcaaggga gacatccgtc ggagaaggcc
     9361 atcctaagaa acgagagatg gcacaggccc cagaaggaga aggaaaaggg aacccagcga
     9421 gtgaagacgg catggggttg ggtgagggag gagagatgcc cggagaggac ccagacacgg
     9481 ggaggatccg ctcagaggac atcacgtggt gcagcgccga gaaggaagtg ctccggaaag
     9541 agcatccttg ggcagcaaca cagcagagag caaggggaag agggagtgga ggaagacgga
     9601 acctgaagga ggcggcaggg aaggatctgg gccagccgta gaggtgaccc aggccacaag
     9661 ctgcagacag aaagcggcac aggcccaggg gagagaatgc tggtcagaga aagca
//
