LOCUS       sequence-455918-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human RUNX1C gene overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  TITLE     Klusmann Lab plasmids 2022
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2423..2432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2435..2461
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3927..4501,4502..4504,4505..4513)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4094..4111)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4321..4340
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4514..4516,4517..4519,4520..5233)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4514..4516,4517..4519,4520..5230)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4559..4580)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4820..4839)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5167..5188
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5465..5868
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5958..6191
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6263..6384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6300..6319)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6354..6373
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6424..6559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6579..6598)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6580..6598)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6608..6625)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6608..6624)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6617..6639)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6766..7221
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6853..6872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7063..7084
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7247..7351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7352..7420,7421..8212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7570..7589)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8383..8971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8872..8891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9125..9142
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9259..9280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9295..9300,9301..9318,9319..9325)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9333..9349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9338..9360
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9357..9373
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9357..9373
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9392..9412
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9394..9412
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctagctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacggatcc
     2401 accggtacta gtatactccg gagccaccat gggatacccc tacgacgtgc ccgactacgc
     2461 ttcagacagc atatttgagt catttccttc gtacccacag tgcttcatga gagaatgcat
     2521 acttggaatg aatccttcta gggacgtcca cgatgccagc acgagccgcc gcttcacgcc
     2581 gccttccacc gcgctgagcc caggcaagat gagcgaggct ttgcctctgg gcgccccaga
     2641 cgccggcgct gccctggccg gcaagctgag gagcggcgac cgcagcatgg tggaggtgct
     2701 ggccgaccac ccgggcgagc tggtgcgcac cgacagcccc aacttcctct gctccgtgct
     2761 gcctacgcac tggcgctgca acaagaccct gcccatcgct ttcaaggtgg tggccctagg
     2821 ggatgttcca gatggcactc tggtcactgt gatggctggc aatgatgaaa actactcggc
     2881 tgagctgaga aatgctaccg cagccatgaa gaaccaggtt gcaagattta atgacctcag
     2941 gtttgtcggt cgaagtggaa gagggaaaag cttcactctg accatcactg tcttcacaaa
     3001 cccaccgcaa gtcgccacct accacagagc catcaaaatc acagtggatg ggccccgaga
     3061 acctcgaaga catcggcaga aactagatga tcagaccaag cccgggagct tgagcttcag
     3121 cgagagactg agtgaactgg agcagctgcg gcgcacagcc atgagggtca gcccacacca
     3181 cccagccccc acgcccaacc ctcgtgcctc cctgaaccac tccactgcct ttaaccctca
     3241 gcctcagagt cagatgcagg atacaaggca gatccaacca tctcctccct ggagctatga
     3301 ccagtcctac caatacctgg gaagcattgc ctctccttct gtgcacccag caacgcccat
     3361 ttcacctgga cgtgccagcg gcatgacaac cctctctgca gaactttcca gtcgactctc
     3421 aacggcaccc gacctgacag cgttcagcga cccgcgccag ttccccgcgc tgccctccat
     3481 ctccgacccc cgcatgcact atccaggcgc cttcacctac tccccgacgc cggtcacctc
     3541 gggcatcggc atcggcatgt cggccatggg ctcggccacg cgctaccaca cctacctgcc
     3601 gccgccctac cccggctcgt cgcaagcgca gggaggcccg ttccaagcca gctcgccctc
     3661 ctaccacctg tactacggcg cctcggccgg ctcctaccag ttctccatgg tgggcggcga
     3721 gcgctcgccg ccgcgcatcc tgccgccctg caccaacgcc tccaccggct ccgcgctgct
     3781 caaccccagc ctcccgaacc agagcgacgt ggtggaggcc gagggcagcc acagcaactc
     3841 ccccaccaac atggcgccct ccgcgcgcct ggaggaggcc gtgtggaggc cctactgacg
     3901 cgtatgcatg agtaactgag gatccgcccc tctccctccc ccccccctaa cgttactggc
     3961 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     4021 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     4081 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     4141 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     4201 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     4261 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     4321 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     4381 atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa
     4441 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     4501 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4561 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4621 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4681 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4741 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4801 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4861 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4921 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4981 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     5041 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5101 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     5161 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5221 gctgtacaag taaagcggcc gcgtcgacgg atcccccggg ctgcaggaat tcgagcatct
     5281 taccgccatt tattcccata tttgttctgt ttttcttgat ttgggtatac atttaaatgt
     5341 taataaaaca aaatggtggg gcaatcattt acatttttag ggatatgtaa ttactagttc
     5401 aggtgtattg ccacaagaca aacatgttaa gaaactttcc cgttatttac gctctgttcc
     5461 tgttaatcaa cctctggatt acaaaatttg tgaaagattg actgatattc ttaactatgt
     5521 tgctcctttt acgctgtgtg gatatgctgc tttaatgcct ctgtatcatg ctattgcttc
     5581 ccgtacggct ttcgttttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5641 gttgtggccc gttgtccgtc aacgtggcgt ggtgtgctct gtgtttgctg acgcaacccc
     5701 cactggctgg ggcattgcca ccacctgtca actcctttct gggactttcg ctttccccct
     5761 cccgatcgcc acggcagaac tcatcgccgc ctgccttgcc cgctgctgga caggggctag
     5821 gttgctgggc actgataatt ccgtggtgtt gtcggggaag ctgacgtcct ttcgaattcg
     5881 atatcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5941 aaaagaaaag gggggactgg aagggctacg taactcccaa cgaagacaag atctgctttt
     6001 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6061 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6121 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6181 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6241 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6301 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6361 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6421 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6481 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6541 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     6601 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     6661 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     6721 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     6781 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6841 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6901 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6961 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7021 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7081 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7141 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7201 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7261 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7321 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7381 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7441 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7501 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7561 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7621 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7681 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7741 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7801 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7861 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7921 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7981 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8041 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8101 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8161 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8221 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8281 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8341 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8401 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8461 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8521 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8581 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8641 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8701 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8761 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8821 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8881 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8941 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9001 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9061 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9121 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9181 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9241 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9301 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9361 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     9421 gctggagctg ca
//
