LOCUS       sequence-455920-       10648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLVX-TetOne-Puro-mitoLbNOX-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-mitoLbNOX-FLAG
FEATURES             Location/Qualifiers
     source          1..10648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(179..925)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(944..1454)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1007..1025)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        1471..1838
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    1479..1497
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1515..1533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1551..1569
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1587..1605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1623..1641
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1659..1677
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1695..1713
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3294..3317
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3513..3647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3691..3806
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(4069..4113)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4298..4531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5028..5153
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5200..5833
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6001..6135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6183..6287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6288..6356,6357..7148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6506..6525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7319..7907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7808..7827
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8061..8078
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8195..8216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8231..8236,8237..8254,8255..8261)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8269..8285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8274..8296
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8293..8309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8293..8309
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8437..9070
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9277..9865
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9383..9394
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9792..9812
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9879..10478)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9962..9982)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     10459..10478
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(10487..10648,1..168))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10501..10636
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
       61 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
      121 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tcgcgcgttt
      181 acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt
      241 caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg
      301 tcggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt agttccaggc
      361 cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtttcagaa
      421 gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct
      481 gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt
      541 tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact
      601 gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag
      661 cacagcggta tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg
      721 ggcaggagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg agcagggccc
      781 gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt
      841 tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagca gagtttatga
      901 ctttgctctt gtccagtcta gacatggtaa ttcgatgatc cccctgggga gagaggtcgg
      961 tgattcggtc aacgagggag ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg
     1021 aacaccgcgc gggcaggaac agggcccaca ctaccgcccc acaccccgcc tcccgcaccg
     1081 ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca gccgctattg gccacagccc
     1141 atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg gtactagtga
     1201 gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac
     1261 ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg aagatccggg
     1321 tgacgctgcg aacggacgtg aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc
     1381 cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc
     1441 gcaaccccaa ccccgtggaa ttatcacctc gagtttactc cctatcagtg atagagaacg
     1501 tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag
     1561 tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt
     1621 actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg
     1681 tatatccagt ttactcccta tcagtgatag agaacgtata agctttgctt atgtaaacca
     1741 gggcgcctat aaaagagtgc tgattttttg agtaaacttc aattccacaa cacttttgtc
     1801 ttataccaac tttccgtacc acttcctacc ctcgtaaaga attcatgctc gctacaaggg
     1861 tctttagcct cgtcggaaag agagctatca gcacctccgt ctgcgtgaga gctcataagg
     1921 tcaccgtggt cggatgcacc catgccggca ccttcgccat caagcaaatc ctcgctgagc
     1981 accctgacgc cgaggtcacc gtctacgaga ggaacgatgt gatctccttc ctgtcctgtg
     2041 gcatcgccct ctacctgggc ggaaaagtgg ccgatcccca aggcctcttc tacagctccc
     2101 ctgaagaact gcagaagctg ggcgctaatg tgcagatgaa ccacaacgtg ctggccatcg
     2161 accctgacca aaagaccgtc acagtcgagg acctcaccaa tcacgcccag accaccgagt
     2221 cctacgacaa actggtgatg acctccggaa gctggcctat cgtgcccaaa atccccggca
     2281 tcgacagcga tagggtgaag ctctgcaaga attgggccca cgcccaggct ctgattgagg
     2341 acgccaagga ggccaagagg atcaccgtca tcggcgccgg atacatcgga gccgaactgg
     2401 ccgaggccta ctccacaaca ggccacgacg tcaccctgat tgacgccatg gctagggtca
     2461 tgcccaagta cttcgatgcc gacttcaccg acgtcatcga acaggactac agggaccatg
     2521 gcgtgcaact cgctctgggc gagacagtgg agagcttcac cgacagcgcc accggcctca
     2581 caatcaagac agacaagaac tcctatgaga ccgacctggc catcctctgc attggcttta
     2641 ggcccaacac agacctgctg aaaggcaaag tggacatggc ccctaacggc gccatcatta
     2701 ccgacgacta catgaggtcc agcaaccctg atattttcgc tgctggcgac tccgccgccg
     2761 tccattacaa ccccacacac caaaacgcct acattcccct cgctaccaac gccgtcaggc
     2821 agggaatcct cgtcggaaag aacctcgtca agcccacagt gaagtacatg ggaacccagt
     2881 ccagctccgg actggccctc tatgacagga caattgtctc cacaggcctc acactggccg
     2941 ccgccaagca acaaggcctc aatgccgagc aggtcatcgt ggaggacaac tataggcccg
     3001 agttcatgcc ttccaccgag cccgtcctca tgagcctggt cttcgacccc gatacacaca
     3061 gaatcctggg aggcgccctg atgtccaaat acgacgtgtc ccagagcgct aataccctgt
     3121 ccgtctgcat ccagaacgag aacaccatcg atgacctggc catggtggac atgctgttcc
     3181 agcccaattt cgacaggccc ttcaactacc tgaacattct cgcccaggct gcccaagcta
     3241 aagtggccca atccgtcaac gctggtggat ctggtggatc tggtggatct atggattaca
     3301 aggatgacga tgacaagtaa accggtgccg gcggatccag accacctccc ctgcgagcta
     3361 agctggacag ccaatgacgg gtaagagagt gacatttttc actaacctaa gacaggaggg
     3421 ccgtcagagc tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa
     3481 cctaagacag gcgcagcttc cgagggattt gagatccaga catgataaga tacattgatg
     3541 agtttggaca aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg
     3601 atgctattgc cttatttgta accattataa gctgcaataa acaagttaag tcgataaact
     3661 ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
     3721 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     3781 cccccaatcc ccccttttct tttaaaggcg ataccgtcga gatccgttca ctaatcgaat
     3841 ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc
     3901 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     3961 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     4021 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     4081 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     4141 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     4201 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     4261 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     4321 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     4381 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     4441 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     4501 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     4561 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     4621 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
     4681 cggccggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
     4741 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     4801 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     4861 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     4921 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     4981 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     5041 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     5101 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     5161 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     5221 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     5281 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     5341 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct
     5401 gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccct
     5461 ggaaagtccc cagcggaaag tcccttgtag caagctcgat atcagcagtt cttgaagtac
     5521 tccggatgca gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa cctccactct
     5581 aacacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa caagctggtg
     5641 ttctctcctt tattggcctc ttctacctta tctggctcaa ctggtactag cttgtagcac
     5701 catccaaagg tcagtggata tctgacccct ggccctggtg tgtagttctg ctaatcaggg
     5761 aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttct ttgggagtga
     5821 attagccctt ccaactacta agtttgtagt acatatttaa caaatacaat ttctttaaaa
     5881 tgaaaataat tcagaggaat cacaggttta gagtaaatga aaccacaggt aattggcagt
     5941 ggtaataggg tatggggtgg gaagtttggg atgattttgg ttagcttgag ttatccagtt
     6001 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     6061 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6121 tgcaataaac aagttgatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
     6181 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6241 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6301 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6361 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6421 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6481 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6541 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6601 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6661 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6721 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6781 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6841 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6901 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6961 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7021 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7081 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7141 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7201 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7261 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7321 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7381 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7441 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7501 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7561 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7621 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7681 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7741 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7801 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7861 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7921 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7981 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8041 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8101 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8161 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8221 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8281 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg
     8341 acggtaaacg ctagcaatgt caaggcctct cactctctga tattcatttc tttgcaagtt
     8401 ataaatactg aataataaga tgacatgaac tactactgct agagattttc cacactgact
     8461 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     8521 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     8581 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa agcagctgct
     8641 tatatgcagg atctgagggc tcgccactcc ccagtcccgc ccaggccacg cctccctgga
     8701 aagtccccag cggaaagtcc cttgtagcaa gctcgatatc agcagttctt gaagtactcc
     8761 ggatgcagct ctcgggccac gtgatgaaat gctaggcggc tgtcaaacct ccactctaac
     8821 acttctctct ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gctggtgttc
     8881 tctcctttat tggcctcttc taccttatct ggctcaactg gtactagctt gtagcaccat
     8941 ccaaaggtca gtggatatct gacccctggc cctggtgtgt agttctgcta atcagggaag
     9001 tagccttgtg tgtggtagat ccacagatca aggatatctt gtcttcgttg ggagtgaatt
     9061 agcccttcca gtcccctctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa
     9121 gtcattggtc ttaaaggtac ctgaggtgtg actggaaaac ccacctcctc ctcctcttgt
     9181 gcttctagcc aggcacaatc agcattggta gctgctgtat tgctacttgt gattgctcca
     9241 tgtttttcta ggtctcgact gcagaattaa ttccaggcgg ggaggcggcc caaagggaga
     9301 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     9361 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     9421 gcagaatcca ggtggcaaca caggcgagca gccatggaaa ggacgtcagc ttccccgaca
     9481 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     9541 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     9601 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     9661 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     9721 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     9781 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     9841 aaattttgta atccagaggt tgattgttcc agacgcgttc aggcaccggg cttgcgggtc
     9901 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     9961 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
    10021 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
    10081 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
    10141 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
    10201 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
    10261 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
    10321 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccgggtcgac ggtgtggcgc
    10381 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggcccgggg gacgtcgtcg
    10441 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tcgcgttttg caaaagccta
    10501 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
    10561 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
    10621 gagttagggg cgggatgggc ggagttag
//
