LOCUS       sequence-455928-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Beilina A, Van Der Brug M, Ahmad R, Kesavapany S, Miller DW, Petsko 
            GA, Cookson MR
  TITLE     Mutations in PTEN-induced putative kinase 1 associated with 
            recessive parkinsonism have differential effects on protein 
            stability.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8.
  PUBMED    15824318
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Apr 19. 102(16):5703-8."
COMMENT     Sequence Label: pLenti6-DEST PINK1-V5 G309D
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    625..649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             667..2409
                     /codon_start=1
                     /product="human PTEN-induced putative kinase 1, a 
                     mitochondria serine/threonine protein kinase"
                     /label=PINK1
                     /translation="MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGER
                     PGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAF
                     GLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIG
                     KGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMW
                     NISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAF
                     TSSVPLLPGALVDYPDVLPSRLHPEGLDHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAA
                     MMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLP
                     FSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYG*GK
                     AHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHIL
                     ALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCS
                     WRAAL"
     protein_bind    complement(2429..2453)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2506..2547
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2584..2604)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2589..2918
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2769..2904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2966..3013
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3032..3430
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3517..3750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3822..3943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3859..3878)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3913..3932
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3983..4118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4138..4157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4139..4157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4167..4184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4167..4183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4176..4198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4325..4780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4412..4431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4622..4643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4806..4910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4911..4979,4980..5771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5129..5148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5942..6530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6431..6450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6684..6701
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6818..6839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6854..6859,6860..6877,6878..6884)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6892..6908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6897..6919
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6916..6932
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6916..6932
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6951..6971
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6953..6971
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6999..7225
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7226..7406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7453..7578
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8071..8304
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8489..8533
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc actagtccag
      601 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctt gaaggaattc
      661 ggtaccatgg cggtgcgaca ggcgctgggc cgcggcctgc agctgggtcg agcgctgctg
      721 ctgcgcttca cgggcaagcc cggccgggcc tacggcttgg ggcggccggg cccggcggcg
      781 ggctgtgtcc gcggggagcg tccaggctgg gccgcaggac cgggcgcgga gcctcgcagg
      841 gtcgggctcg ggctccctaa ccgtctccgc ttcttccgcc agtcggtggc cgggctggcg
      901 gcgcggttgc agcggcagtt cgtggtgcgg gcctggggct gcgcgggccc ttgcggccgg
      961 gcagtctttc tggccttcgg gctagggctg ggcctcatcg aggaaaaaca ggcggagagc
     1021 cggcgggcgg tctcggcctg tcaggagatc caggcaattt ttacccagaa aagcaagccg
     1081 gggcctgacc cgttggacac gagacgcttg cagggctttc ggctggagga gtatctgata
     1141 gggcagtcca ttggtaaggg ctgcagtgct gctgtgtatg aagccaccat gcctacattg
     1201 ccccagaacc tggaggtgac aaagagcacc gggttgcttc cagggagagg cccaggtacc
     1261 agtgcaccag gagaagggca ggagcgagct ccgggggccc ctgccttccc cttggccatc
     1321 aagatgatgt ggaacatctc ggcaggttcc tccagcgaag ccatcttgaa cacaatgagc
     1381 caggagctgg tcccagcgag ccgagtggcc ttggctgggg agtatggagc agtcacttac
     1441 agaaaatcca agagaggtcc caagcaacta gcccctcacc ccaacatcat ccgggttctc
     1501 cgcgccttca cctcttccgt gccgctgctg ccaggggccc tggtcgacta ccctgatgtg
     1561 ctgccctcac gcctccaccc tgaaggcctg gaccatggcc ggacgctgtt cctcgttatg
     1621 aagaactatc cctgtaccct gcgccagtac ctttgtgtga acacacccag cccccgcctc
     1681 gccgccatga tgctgctgca gctgctggaa ggcgtggacc atctggttca acagggcatc
     1741 gcgcacagag acctgaaatc cgacaacatc cttgtggagc tggacccaga cggctgcccc
     1801 tggctggtga tcgcagattt tggctgctgc ctggctgatg agagcatcgg cctgcagttg
     1861 cccttcagca gctggtacgt ggatcggggc ggaaacggct gtctgatggc cccagaggtg
     1921 tccacggccc gtcctggccc cagggcagtg attgactaca gcaaggctga tgcctgggca
     1981 gtgggagcca tcgcctatga aatcttcggg cttgtcaatc ccttctacgg ctagggcaag
     2041 gcccaccttg aaagccgcag ctaccaagag gctcagctac ctgcactgcc cgagtcagtg
     2101 cctccagacg tgagacagtt ggtgagggca ctgctccagc gagaggccag caagagacca
     2161 tctgcccgag tagccgcaaa tgtgcttcat ctaagcctct ggggtgaaca tattctagcc
     2221 ctgaagaatc tgaagttaga caagatggtt ggctggctcc tccaacaatc ggccgccact
     2281 ttgttggcca acaggctcac agagaagtgt tgtgtggaaa caaaaatgaa gatgctcttt
     2341 ctggctaacc tggagtgtga aacgctctgc caggcagccc tcctcctctg ctcatggagg
     2401 gcagccctga agctcgagtg cggccgcaac ccagctttct tgtacaaagt ggttgatatc
     2461 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     2521 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagtttg gaattaattc
     2581 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     2641 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     2701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     2761 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2821 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     2881 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     2941 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     3001 agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc
     3061 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     3121 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     3181 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     3241 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     3301 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     3361 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     3421 ggagggctaa gcacaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     3481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3721 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     3781 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3961 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4021 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4081 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4141 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4201 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4261 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4321 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4381 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4621 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4681 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4741 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4801 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4861 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4921 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4981 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5041 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5101 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5161 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5221 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5281 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5341 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5401 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5461 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5521 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5581 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5641 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5701 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5761 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5821 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5881 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5941 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6001 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6061 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6121 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6181 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6241 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6301 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6361 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6421 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6481 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6541 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6601 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6661 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6721 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6781 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6841 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6901 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6961 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     7021 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7081 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7141 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7201 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7381 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7441 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7501 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7561 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7621 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7681 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7741 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7801 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7861 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7921 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7981 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8041 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8101 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8161 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8221 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8281 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8341 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8401 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8461 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8521 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8581 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8641 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8701 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     8761 cattcgatta gtgaacggat ctcgacggta tcgataagct tgggagttcc g
//
