LOCUS       sequence-455971-        4348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPMS0CM0031
FEATURES             Location/Qualifiers
     source          1..4348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2391..2407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2391..2407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2404..2426)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2579..3167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2659..2678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3300..3394)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3415..4074)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3989..4008
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4075..4177)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4264..4286
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4278..4295
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4279..4295
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aggtctctaa tgtggaaact caggattggc gaaaaaaacg gcaaatttaa catcggtgac
       61 gggaacggag atgactatct gcacagtact aacaacttcg tcggtcgtca aacgtgggaa
      121 ttcgatccag acgccggaac gcaaaaggag cgagatgaaa tagaacgtct tagggaacag
      181 ttcctgataa ataaaaaaaa attggatatc aggtgctgcg ccgacttatt aatgaggaac
      241 caactcatca aagaaagtgg aatagattta atcagtgtac cacccgttcg tttaggggat
      301 gatgaggacg taaattatga agcagtgacg acagccgttc gtaaagctgt gcgtctgaat
      361 agagctatac aggcctggga cggacattgg cccgctgaaa atgctggccc gttattcttc
      421 actccgccat taattatagc attgtacatc tccgggacct tagatactat actgactcaa
      481 gagcacaagc gagaaatgat ccgatatatg tatatacacc aaaatgaaga tggaggctgg
      541 ggattttaca tatccgggcg tagcactatg atagggacag cactcaatta cgtcggactg
      601 aggttactgg gcgaagatga taatgatgcc attgcaaagg gtcgaaagtg gatccttgat
      661 cacggagggg caacatcaat tccaagttgg ggcaaggttt acctctccgt gcttggcgtc
      721 tacgaatggg ctggctgtaa cccattacca ccagagtttt ggttgttccc cagttttctc
      781 ccctaccacc cagctaagat gtggtgttat tgtcgaacta cctacatgcc gatgagctac
      841 ctctacggga caggattcca gggtcccata actgatctgg taaaatctct gaggaaagag
      901 attcatgtta tcccttacca ccagattgac tggaataaac aaagacacaa ctgttgtaaa
      961 gaagatctgt actatccgca tacatacata caggacctct tgtgggatgg gttacattac
     1021 ttctctgagc ccctcgttag taagtggcct cttaaaaagt tacgtgagaa gggcttgaag
     1081 agggtccttg acttgatgca atataacgcc gaagaaggta gatacataac attggggtgt
     1141 gtcgagaaat ccttacaaat gatgtgcttc tcagctcttg acccaaatgg tatcgatttt
     1201 aaaagacacc tggcccgagt gcctgactac ctctgggtcg ctgaggacgg gatgaagatg
     1261 caatctttcg ggagtcagtt gtgggactgt acgctggtaa cgcaagcaat aatcgcctcc
     1321 gacatggtcg aagaatatgg cgactcttta aagaaagcta atttctactt aaaagagtca
     1381 caaataaagc aaaaccccaa gggggacttt gagaatatgt gtaggcaatt taccaaagga
     1441 gcttggacat ttagcgacca ggaccaaggc tgggttgtta gtgattgcac tgctgaagca
     1501 gttaaatgcc tgctcgcttt gtctcagatg cctcaggaaa tttctggtga gaaagtggac
     1561 gttgagcgat tgtatgacgc tattaacgtc cttttgtatt tgcaaagtcc agagactggg
     1621 gggttcgcca tctgggaagc tccggtgcct aagccatacc tcgaaaagct caacccctct
     1681 gaactttttg ctgacatagt agtagaacgt gagcacgtag agtgcacagg gagtataatt
     1741 cagactctcc agactttcaa gacactgcac ccgggccatc gtcagaaaga aatcgaggtg
     1801 gctattgaga aaggaatcag gtttctcgag aataggcagc aagagaacgg atcatggtac
     1861 ggatattggg gtatatgtta cttgtacgga acgtatttcg tgctgcaagg actggtggcc
     1921 tgtggacaga cgtatgagaa ctccgaagcc gttagaaaag ctgttaaatt tttcctttca
     1981 acacaaaatt cagagggtgg ctggggagaa aatttcgagt cttgccctca ggaaaaattt
     2041 atcccacttg agggaaaccg aacaaatttt gtacagacct cttgggccat gttagggttg
     2101 ctgtgtggag ggcaagcaga aagggatccg acacctcttc acaaagctgc taagctgctt
     2161 attaatggtc aaatggacaa cggggacttc cctcagcaag aaatcactgg ggtttatatg
     2221 aagaattgca tgctgcacta cgccgaatat cgaaacacct tccctttatg ggctttaggc
     2281 gaataccgta aacgagtgtg gctggcaaaa caagagactg gttcgagaga cctaaggcta
     2341 ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg
     2401 tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca
     2461 atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca
     2521 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2581 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2641 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2701 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2761 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2821 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2881 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2941 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3001 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3061 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3121 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3181 tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta
     3241 aatgggtagg gggcttcaaa tcgtcccccc atacgatata agttgttact agtgcttgga
     3301 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     3361 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     3421 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     3481 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     3541 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     3601 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     3661 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     3721 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     3781 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     3841 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     3901 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     3961 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     4021 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     4081 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     4141 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattgat acctgaaaca
     4201 aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt
     4261 tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga
     4321 ataaggaagt gccattccgc ctgacctt
//
