LOCUS       sequence-455972-        4348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPMS0CM0032
FEATURES             Location/Qualifiers
     source          1..4348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2392..2408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2392..2408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2405..2427)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2580..3168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2660..2679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3301..3395)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3416..4075)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3990..4009
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4076..4178)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4265..4287
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4279..4296
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4280..4296
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgtggaagt tacgaatagg tgagaaaaac ggaaaattca acatcgggga
       61 cgggaacggc gacgactacc ttcatagcac aaataacttt gtcgggaggc aaacttggga
      121 gttcgatcca gacgccggca cacaagaaga gagagatgaa atagagaggt ttcgtgagca
      181 attcttaatt aataagaaaa agcttgacat ctcatgctgt gccgatttgt taatgcgaaa
      241 tcagcttata aaggaaagtg gaatcgattt aataagcgtg ccgccggtga ggcttggcga
      301 cgacgaagat gtcaactacg aagctgtaac aacggcagtc cgtaaagccg tccgattgaa
      361 ccgtgccata caagcatggg atggccattg gccggcagaa aatgcaggac cccttttctt
      421 tactcctccc ctcatcatcg ctatgtacat atccggaacg ctggatacga ttttgactca
      481 agagcacaag cgagaaatga tacgttatat gtatatccat cagaacgaag atggaggctg
      541 gggtttttac atttcaggca ggagtacgat gattggaact gcactgaatt acgtcggact
      601 gaggctcctg ggcgaggatg ataatgatgc catagccaaa ggtcgaaaat ggattctcga
      661 ccatggaggc gcaacatcta tcccgagctg gggcaaggta tacatctccg tgcttggagt
      721 ttacgaatgg gctggctgta accctttacc acctgagttt tggctgtttc ctagtttttt
      781 accgtatcat ccagctaaaa tgtggtgtta ttgtagaacc acctatatgc caatgtccta
      841 tttatatgga cgtgggttcc aaggacccat caccgacctg gtaaagagtc tccgaaaaga
      901 aattcatgtc acaccgtatc atcaaattga ttggaataaa caacgacata actgctgtaa
      961 agaagattta tactacccac atacctatat acaggacctc ctctgggatg ggcttcacta
     1021 cttcagcgaa cccctggttt caaaatggcc gttcaaaaag ttgcgtgaga aagggttaaa
     1081 acgagtgctg gacttaatgc agtacaatgc agaggaaggg cgttacatca caatggggtg
     1141 cgttgaaaaa gcactgcaaa tgatgtgttt ttatgcctta gatcccaatg gtgtcgattt
     1201 caagagacac ttagctaggt taccggatta tttgtgggtt gctgaagatg ggatgaaaat
     1261 gcaatctttc ggctctcagc tgtgggactg cacactcgtc actcaagcca taattgcaag
     1321 tgacatggtt gaggaatatg gggactcctt gaagaaagcc aacttttatt tgaaggaaag
     1381 tcaaataaaa cagaacccca agggggactt tgagaacatg tgtcgacaat ttactaaagg
     1441 cgcctggact ttctctgacc aagaccaggg gtgggtcgta agcgactgca ctgctgaagc
     1501 agttaagtgc ttgttagccc tctcacagat gccacaagag atcagtggtg aaaaggtaga
     1561 cgtggaaaga ctgtacgacg ccattaatgt tctgctctat ctgcaaagcc ccgagactgg
     1621 aggtttcgct atctgggagg ctccagtgcc gaagccgtat cttgaaaaac ttaaccccag
     1681 tgagctcttt gccgacatcg tagtcgaacg tgaacatgtt gagtgcaccg gttccattat
     1741 tcaggcactc cagaccttca agacgctgca cccgggtcat cgtcaaaagg agattgaggt
     1801 tgccatcgaa aaaggaatac gattcttgga aaacaggcaa caggagaatg ggagttggta
     1861 cggttactgg ggcatatgtt atttatatgg aacgtacttc gttttgcaag gactggttgc
     1921 ctgtggacag acatacgaaa actccgaggc cgtcaggaag gctgtaaaat tcttcctgtc
     1981 tacgcaaaac agcgaagggg gctggggaga gaatttcgaa agttgcccgc aagaaaaatt
     2041 tataccactc gaagggaacc gtacgaattt agttcagact agctgggcca tgctcggtct
     2101 gctgtgtgga ggtcaggcag agcgtgatcc tacccccctg cacaaggcag caaagcttct
     2161 cattaacggg cagatggata acggagactt cccgcaacaa gagatcacag gggtctacat
     2221 gaaaaattgt atgctgcact acgcagagta taggaacacc tttccccttt gggcactcgg
     2281 agagtacaga aaaagggttt ggctgccgaa gcaggagaca ggttcgagag acctaaggct
     2341 aggtggaggc tcagtgatga taagtctgcg atggtggatg catgtgtcat ggtcatagct
     2401 gtttcctgtg tgaaattgtt atccgctcag agggcacaat cctattccgc gctatccgac
     2461 aatctccaag acattaggtg gagttcagtt cggcgtatgg catatgtcgc tggaaagaac
     2521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3181 atcttttcta cggggtctga cgctctattc aacaaagccg ccgtcccgtc aagtcagcgt
     3241 aaatgggtag ggggcttcaa atcgtccccc catacgatat aagttgttac tagtgcttgg
     3301 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     3361 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     3421 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     3481 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     3541 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     3601 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     3661 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     3721 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     3781 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     3841 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     3901 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     3961 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     4021 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     4081 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     4141 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcattga tacctgaaac
     4201 aaaacccatc gtacggccaa ggaagtctcc aataactgtg atccaccaca agcgccaggg
     4261 ttttcccagt cacgacgttg taaaacgacg gccagtcatg cataatccgc acgcatctgg
     4321 aataaggaag tgccattccg cctgacct
//
