LOCUS       sequence-455988-        6142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Burkholderia allelic exchange suicide vector pMo130, ref EU862243.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei.
  JOURNAL   Gene. 2009 Feb 1. 430(1-2):123-31.
  PUBMED    19010402
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2009 
            Feb 1. 430(1-2):123-31."
COMMENT     Sequence Label: pMo130
FEATURES             Location/Qualifiers
     source          1..6142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(756..761,762..777,778..784)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     1827..1849
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1887..1905)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1973..2076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2077..2871
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2131..2150)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2741..2760
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    2930..2946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      4438..4483
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     oriT            5734..5843
                     /note="incP origin of transfer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg atatcagggc
      721 cccgctagcc agatcttccc gggaagcttg agctgttgac cattaatcat cggctcgtat
      781 aatgtgtgga atatcacaga aggagacaga atactatgaa caaaggtgta atgcgaccgg
      841 gccatgtgca gctgcgtgta ctggacatga gcaaggccct ggaacactac gtcgagttgc
      901 tgggcctgat cgagatggac cgtgacgacc agggccgtgt ctatctgaag gcttggaccg
      961 aagtggataa gttttccctg gtgctacgcg aggctgacga gccgggcatg gattttatgg
     1021 gtttcaaggt tgtggatgag gatgctctcc ggcaactgga gcgggatctg atggcatatg
     1081 gctgtgccgt tgagcagcta cccgcaggtg aactgaacag ttgtggccgg cgcgtgcgct
     1141 tccaggcccc ctccgggcat cacttcgagt tgtatgcaga caaggaatat actggaaagt
     1201 ggggtttgaa tgacgtcaat cccgaggcat ggccgcgcga tctgaaaggt atggcggctg
     1261 tgcgtttcga ccacgccctc atgtatggcg acgaattgcc ggcgacctat gacctgttca
     1321 ccaaggtgct cggtttctat ctggccgaac aggtgctgga cgaaaatggc acgcgcgtcg
     1381 cccagtttct cagtctgtcg accaaggccc acgacgtggc cttcattcac catccggaaa
     1441 aaggccgcct ccatcatgtg tccttccacc tcgaaacctg ggaagacttg cttcgcgccg
     1501 ccgacctgat ctccatgacc gacacatcta tcgatatcgg cccaacccgc cacggcctca
     1561 ctcacggcaa gaccatctac ttcttcgacc cgtccggtaa ccgcaacgaa gtgttctgcg
     1621 ggggagatta caactacccg gaccacaaac cggtgacctg gaccaccgac cagctgggca
     1681 aggcgatctt ttaccacgac cgcattctca acgaacgatt catgaccgtg ctgacctgag
     1741 cggccgccct gcagcggatc cctctagatg catgcgacgg gcttgtctgc tcccggcatc
     1801 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     1861 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1921 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1981 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2041 ctgataaatg cttcaataat ttgaaaaagg aagagtatga ttgaacaaga tggattgcac
     2101 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     2161 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     2221 gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg
     2281 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     2341 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     2401 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     2461 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     2521 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     2581 gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccat
     2641 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     2701 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     2761 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     2821 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg attaattaaa
     2881 ctagtgagct gttgacaatt aatcatcgaa tctagtttaa tgtgtggaat tgtgagcgga
     2941 taacaattaa gcgttaggag gaaacgatga cgtactccaa acaagccgtt tcccgtagac
     3001 acaaacgttt ggccctgagc gccgccgcgc tcaccgcagc cgcttgcatg tcggcgcacg
     3061 cgcagagcga cggcgcaact caagcgtttg cggaagaaac gaaccaaaag ccatataagg
     3121 aaacatacgg catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa
     3181 aaaatgaaaa atatcaagtt tctgaatttg attcgtccac aattaaaaat atctcttctg
     3241 caaaaggcct ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa
     3301 actatcacgg ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca
     3361 catcgattta catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg
     3421 ctggccgcgt ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc
     3481 aaacacaaga atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct
     3541 acactgattt ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg
     3601 tatcagcatc agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg
     3661 acggtgacgg aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct
     3721 caggcgacaa ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact
     3781 tagtatttga agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta
     3841 acaaagcata ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc
     3901 aaagcgataa aaaacgcccg gctgagttag caaacggcgc tctcggtatg attgagctaa
     3961 acgatgatta cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag
     4021 atgaaattga acgcgcgaac gtctttaaaa tgaacggcaa atggtacctg ttcactgact
     4081 cccgcggatc aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt
     4141 atgtttctaa ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa
     4201 aaatggatct tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag
     4261 cgaaaggaaa caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca
     4321 aacaatcaac gtttgcgccg agcttcctgc tgaacatcaa aggcaagaaa acatctgttg
     4381 tcaaagacag catccttgaa caaggacaat taacagttaa caaataatag tagctttcaa
     4441 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttaatctgag aattccgcaa
     4501 ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct
     4561 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     4621 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     4681 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     4741 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     4801 agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt
     4861 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     4921 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     4981 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     5041 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     5101 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
     5161 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
     5221 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     5281 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     5341 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     5401 cagacatctg ccgcggagga acgcaaccgc agcctcatca cgccggcgct tcttggccgc
     5461 gcgggattca acccactcgg ccagctcgtc ggtgtagctc tttggcatcg tctctcgcct
     5521 gtcccctcag ttcagtaatt tcctgcattt gcctgtttcc agtcggtaga tattccacaa
     5581 aacagcaggg aagcagcgct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     5641 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     5701 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
     5761 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     5821 tcctgctctg cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc
     5881 tgatgaaacc aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga
     5941 cgaacgaaga gcgattgagg attaattaat aactgtcaga ccaagtttac tcatatatac
     6001 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6061 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6121 tagaaaagat caaaggatct tc
//
