LOCUS       sequence-455997-        9400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in BL21(DE3) of T4 primase and helicase fusion with 24 
            amino acid linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Manosas M, Spiering MM, Zhuang Z, Benkovic SJ, Croquette V
  TITLE     Coupling DNA unwinding activity with primer synthesis in the 
            bacteriophage T4 primosome.
  JOURNAL   Nat Chem Biol. 2009 Dec;5(12):904-12. doi: 10.1038/nchembio.236. 
            Epub 2009 Oct 18.
  PUBMED    19838204
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.236"; journalName: "Nat Chem Biol"; date: 
            "2009-12"; volume: "5"; issue: "12"; pages: "904-12"
COMMENT     Sequence Label: pTBY1-T4gp61/41 fusion
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(51..98)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     94..112
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(177..332)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             complement(442..453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(909..1727)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     RBS             4258..4280
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    4295..4319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4319..4338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4320..4338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(4473..4559)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(4656..4742)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        4895..4972
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4973..6055
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4992..5011)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             6612..6803
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6797..6819)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6960..6979
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7001..7018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7172..7760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7252..7271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      7871..8384
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7958..7977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8168..8189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(8426..9217,9218..9286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9049..9068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9287..9391)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgacg tagttatccg gatatagttc ctcctttcag caaaaaaccc
       61 ctcaagaccc gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca
      121 gccaactcag cttcctttcg ggctttgtta gcagccggat ccccttcctg cagtcattga
      181 agctgccaca aggcaggaac gttggatggt tcccatcctg ccaaggaggt gtggggctgc
      241 aaacatttat acgtcttgcc gttatatgtg accaattgtc ccgcagtata agctgtgttg
      301 acctgccaag cggatacacc aggatttgtc gtgaggcctg agttcagacc ggtcaggcca
      361 ccgcatgcat ggacgacaac ctgggatcca agcaaaaact gatgatcaga atcatcagat
      421 aaagtaatcc cataataatc gtcttccttc aattcttgta actcgaaata aaatccgcgg
      481 cactcacgtg caaaagcagc ggcgggagca ggcctgaatt ttttagagcc ggcacacttc
      541 gaaagaacgt taagcaaaac atctccacca gacatataaa tagcataact aattttatgt
      601 ttggtgacat tcatgtcaac cttagcaggt tctgcgttaa ccgagactac taagcctaaa
      661 gaacgagcaa gggaaaccaa accatctctg acagaagtat gaattgtctt tattgttgct
      721 ttaataccat gctcatcagt aacatagcca tcagaatcaa ttagaccagc aagaaatgtt
      781 tcacgagtac cgatattgtc cgtagacaag aaagaaggaa tatttttgac accgtccttc
      841 aagaatccta agccaacaat agcgtcccat aatggattct cagtattaag attattgcga
      901 ataccattac ctctgacaac tttagagtac aaattaacag ttttggcaac ttgtggttct
      961 tttctgtcct tatactcggc gcacaaattc aacttttcag catattcagt aacacgttcc
     1021 atcaaagaag tatctctgga atcaaccgaa aaagttgccc tgtcagacaa tccatcacca
     1081 atccataaac caagtaaata agcaagtact tttggacctt caatggtgag atgaaactta
     1141 cttttttgca tgtagtcgaa aaagtggtca ttctcataaa gaattggagc gtaagtctgg
     1201 taggtagctt tacgaacatg ggaacccaac agagaaagat ctctggcctc aatagtccac
     1261 tcaaaataag ctttatttga agcctttcta taggattcta ctaattcgtt ggctctctca
     1321 ggcccctcag atattgggta gctctttgaa acttccttga caagctcaac aattctaccg
     1381 tcgggggctt tcttttggcc catctcaaaa gtaataactt caaaatattc gacaccctta
     1441 atggtacgag acaaacggcg gacactacga ggtgttctaa caaccaactc atgggtcgca
     1501 ttacacgtaa acttgagtaa ttctggcact tcacgacttg agtcactttt gtgggctctg
     1561 tgctgacttt tctgcacgac gctgtacata gtttctcttc ctctgggcaa tttaattacc
     1621 tcacgaggtc tgccatcttt acccatgacc ttattaccaa cctcaatgtt ttcaatacat
     1681 tcaatagacc catccgccat taaaacattg gtacccttgg caaagcaaaa ttttaattca
     1741 ttcgccaaag catctaactg agctcgagtc gattcatttc tttgatagcg attctgctca
     1801 gcctgaatct gttgtgaacc tgctacttcg ttcacttcag ttggagtaga atcttgttca
     1861 atttctaccc atttttgatt tcctttttga acacccatca aaaacttatt ccacttattc
     1921 ttatcaccat atcgtgattt gatttgctta atgagttgtt gttcagcagc tgctagctcc
     1981 tcggtttcaa tgaccgcaag cataaaatcg gctgttgctg gaagaccggc agattctgca
     2041 atatcgctca tgttaacatc ggaagagtcc caagcttgtt taccaacctg tgctgcagtc
     2101 caaagaacag tttcggtttc aacagccaga gcacgtaatt cctctgcaat agctttaaca
     2161 gttgtgtaac tattttctga ataaactcta atgcggcaag atttacaaat acctagatag
     2221 tcgacaataa tgattgttgg aacaaaattc ttcttgagct tcaattcgtt taaaagtgat
     2281 cgaaatgtat tagcgtctgc tccaccagta ggatactgtt taacgattaa acgaccaaga
     2341 gtagatttct cacgccattt ttccattttt cctttatact cagcgtaaga aatatgccca
     2401 tcatcaatgt catcaagaga aacatcaagc atattagcgt caatacgttt agcacagact
     2461 tcttctgcca tttccatgga aatgtaaaga acattatgtc cgagctgtaa ataatctgcc
     2521 gctaatgaac acaatcctaa tgatttacca acgttaacgc cagccattaa aacgttcagt
     2581 gttccagttt cagctccgcc tttcgtaatt ttgttcagaa ttctgagttt aaatggaacc
     2641 ttacgagctt tattcatata agatagccaa cgtgcttcgt agtcatccat ccaatcatga
     2701 ccaacgtaac tatcaaatga aattgataat gcctggcgca tgatgtcagg aatagcacca
     2761 acatccggca ttttcttatt tcgtttttcc ggaggaagct cagcattagt ttgaatttcg
     2821 attattttag acgtagcatt aaacatcgcc ctttgctgaa catatttttc tgtttctttt
     2881 actaaccagc tgtggtcttc cggagaatca gccagttttg aaataagtgt ttttacacca
     2941 gaatattctg tttcagtaaa tgaactattt tctaatgcaa catttaacgc attaatagat
     3001 ggaacgctat ggtactcatt aacatgagat ttaattaatt tgaatgtatt tttagctgga
     3061 ccactttcaa aatattctga atccatatat ggccaaactt ttgaaaaata agcttgatca
     3121 aatatgagat gagaaagaat aatttctacc atgctaccac caccttcgct accaccaccg
     3181 gtaccaccac cttcaccgct accaccacct tcgctaccac caccaccctt agcatattta
     3241 gatagccgca ttttagccat taacccctgg gcaatattat ttttcatata ttccataatt
     3301 tgttcagggg ttgcaccttc ctttctaatc atatcattaa catcttttga tttccaggga
     3361 gatttatccc aaaacataac cctttctcct gcatcaacta atttagtcat tcgtttaata
     3421 gtgtcagggt gacgaggttc attatctaag acccacacac gtctatcttt aaatggaaca
     3481 acttctaggt ctaattgacc gcccgtaata gctataccat tttcaataaa aagtgaatct
     3541 ataggtcctt ctagaacata tacatcacca tctttaactc gttcgactcc atagattttt
     3601 gttgcctcag gataagcttt gatggtgata tatttttgag gagcatcttt ctttaatgca
     3661 cgtccttgaa aagactcagc ttttccatta gcattataaa ttggaataac aagacgaggc
     3721 tcagaaattt cctttttgta tgttcccggt gctatgctat taactaattt aggccattcg
     3781 gttgtaaacc aaagatattt ccatttatcc tttggaatac aacgagcttt tacgtatttt
     3841 ataattggat ggtcttccgc cagtttatct aatctaacac atgacggaag agatttaatt
     3901 attttcttct cgggttgttt aggaagttct ttaggttttt ctattggacg acttttacct
     3961 ttttcttttc ttatttcaaa gatatactca cgatataaat cgggttcaaa ctcctttaaa
     4021 tatattccga ttggtgcatg atagttacag ttataacaat gaatatttcc ttcattatta
     4081 tcaccataat accatccacg ggctttattc tggtcggttt ttgaatctcc acaaacaggg
     4141 catctaaacc gtaatttaaa agttgaacta ttatttactt gtgtgaattt aggtaaatga
     4201 gctaatgcac ggtatgcaaa ctcattatca atccaaggta ttgatgacat atgtatatct
     4261 ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
     4321 ctatagtgag tcgtattaat ttcgcgggat cgagatcccc ggtttaaacc gggaattaat
     4381 tccgatcccc aattcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
     4441 tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
     4501 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga
     4561 tgcctgggga attaattcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     4621 cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     4681 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     4741 tgatgcctgg ggaattggga attaattctt gaagacgaaa gggcggcatg ccggcatgca
     4801 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     4861 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
     4921 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
     4981 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
     5041 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
     5101 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
     5161 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
     5221 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
     5281 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
     5341 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
     5401 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
     5461 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     5521 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     5581 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     5641 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     5701 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     5761 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     5821 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     5881 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     5941 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6001 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6061 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga
     6121 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     6181 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     6241 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     6301 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     6361 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     6421 cgttcggctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
     6481 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
     6541 tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca
     6601 cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa
     6661 gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca
     6721 tctgtgaatc gctgcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg
     6781 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6841 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6901 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     6961 ggcatcagag cagattgtac tgagagtgca ccatagcttc ctcgctcact gactcgctgc
     7021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7801 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7861 agatcctttt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7921 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7981 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8041 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     8101 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8161 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     8221 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8281 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaat atttgcttat
     8341 acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtaaatca atctaaagta
     8401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     9061 cacatagcag aactttaaaa gtgctcatca ttgggaaacg ttcttcgggg cgaaaactct
     9121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc
//
