LOCUS       sequence-45601-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB542
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    128..144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             196..207
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcaccattcg atggtgtcaa
       61 cgtaaatgca tgccgcttcg ccttcgcgcg cgaattgtcg agaaaattta tcaaaaagag
      121 tgttgacttg tgagcggata acaatgatac ttagattcaa ttgtgagcgg ataacaattt
      181 cacacagaat tcattaaaga ggagaaagga tccatgggta aaataattgg tatcgacctg
      241 ggtactacca actcttgtgt agcgattatg gatggcacca ctcctcgcgt gctggagaac
      301 gccgaaggcg atcgcaccac gccttctatc attgcctata cccaggatgg tgaaactcta
      361 gttggtcagc cggctaaacg tcaggcagtg acgaacccgc aaaacactct gtttgcgatt
      421 aaacgcctga ttggtcgccg cttccaggac gaagaagtac agcgtgatgt ttccatcatg
      481 ccgttcaaaa ttattgctgc tgataacggc gacgcatggg tcgaagttaa aggccagaaa
      541 atggcaccgc cgcagatttc tgctgaagtg ctgaaaaaaa tgaagaaaac cgctgaagat
      601 tacctgggtg aaccggtaac tgaagctgtt atcaccgtac cggcatactt taacgatgct
      661 cagcgtcagg caaccaaaga cgcaggccgt atcgctggtc tggaagtaaa acgtatcatc
      721 aacgaaccga ccgcagctgc gctggcttac ggtctggaca aaggcactgg caaccgtact
      781 atcgcggttt atgacctggg tgg
//
