LOCUS       sequence-456022-        6377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative control plasmid encoding yfp downstream of an attenuated 
            ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli 
            and chloramphenicol resistance to S. aureus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Kviatkovski I, Zhong Q, Vaidya S, Grundling A
  TITLE     Identification of novel genetic factors that regulate c-di-AMP 
            production in Staphylococcus aureus using a riboswitch-based 
            biosensor.
  JOURNAL   mSphere. 2024 Sep 17:e0032124. doi: 10.1128/msphere.00321-24.
  PUBMED    39287429
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere. 
            2024 Sep 17:e0032124. doi: 10.1128/msphere.00321-24."
COMMENT     Sequence Label: pCN38-yfp
FEATURES             Location/Qualifiers
     source          1..6377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..117
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(155..173)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        241..345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(346..414,415..1206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(564..583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1377..1965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1866..1885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3098..3115)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3098..3114)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3107..3129)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
       61 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      121 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
      181 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
      241 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      301 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      361 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      421 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      481 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      541 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      601 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      661 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      721 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      781 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      841 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      901 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      961 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1021 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1081 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1141 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1201 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1261 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1321 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1381 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1441 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1501 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1561 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1621 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1681 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1741 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1801 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1861 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1921 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1981 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2041 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctggcgg ccgctgcatg
     2101 cctgcaggtc gactctagag gatccgtagc ccttgcctac ctagcttcca agaaagatat
     2161 ccttacagca caagagcgga aagatgtttt gttctacatc cagaacaacc tctgctaaaa
     2221 ttcctgaaaa attttcgaaa aagttgttga ctttatctac aaggtgtggc ataatgtgtg
     2281 tgcataatca aacacgaacg ggggaaccaa aagcttaagg aggaaagtca cattatgagc
     2341 aaaggtgaag aactgttcac cggcgttgtg ccaattctgg ttgagctgga tggtgacgtg
     2401 aatggccaca aattttccgt gtctggtgaa ggcgagtgtg atgctactta tggcaaactg
     2461 actctgaaac tgatctgtac caccggcaaa ctgcctgttc cgtggccaac tctggtcact
     2521 actctgggtt acggcctgat gtgttttgcg cgttacccgg atcacatgaa acagcatgac
     2581 ttcttcaaat ctgccatgcc ggaaggctat gtccaagaac gtacgatctt tttcaaggac
     2641 gacggcaact ataaaacccg tgccgaagtt aaattcgagg gtgacaccct ggtcaaccgc
     2701 atcgaactga aaggcattga cttcaaagag gacggcaaca ttctgggtca caagctggaa
     2761 tacaactaca actcccacaa cgtttacatt actgctgaca agcagaaaaa cggcatcaaa
     2821 gcaaacttca agatccgtca caacattgaa gatggtggcg tacagctggc agatcactac
     2881 cagcagaaca ctccaatcgg tgatggccca gtactgctgc cagataacca ttacctgtcc
     2941 taccagagca aactgtctaa agacccgaac gaaaaacgtg accacatggt actgctggaa
     3001 tttgttaccg cggcaggcat tacccacggt atggacgaac tgtataaata agctagcaaa
     3061 aaccccgccc ctgacagggc ggggtttttt tgaattcact ggccgtcgtt ttacaacgtc
     3121 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     3181 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     3241 tgaatggcga atggcgcctt tgcggaaaga gttaataagt taacagaaga tgaaccaaaa
     3301 ctaaatggtt tagcaggaaa cttagataaa aaaatgaatc cagaattata ttcagaacag
     3361 gaacagcaac aagaacaaca aaagaatcaa aaacgagata gaggtatgca cttatagaac
     3421 atgcatttat gccgagaaaa cttattggtt ggaatgggct atgtgttagc taacttgtta
     3481 gcgagttggt tggacttgaa ttgggattaa tcccaagaaa gtaccaactc aacaacacat
     3541 aaagccctgt aggttccgac caataaggaa attggaataa agcaataaaa ggagttgaag
     3601 aaatgaaatt cagagaagcc tttgagaatt ttataacaag taagtatgta cttggtgttt
     3661 tagtagtttt aactgtttac cagataatac aaatgcttaa ataaaaaaag acttgatctg
     3721 attagaccaa atcttttgat agtgttatat taataacaaa ataaaaagga gtcgctcacg
     3781 ccctgaccaa agtttgtgaa cgacatcatt caaagaaaaa aacactgagt tgtttttata
     3841 atcttgtata tttagatatt aaacgatatt taaatataca tcaagatata tatttgggtg
     3901 agcgattcct taaacgaaat tgagattaag gagtcgattt tttatgtata aaaacaatca
     3961 tgcaaatcat tcaaatcatt tggaaaatca cgatttagac aatttttcta aaaccggcta
     4021 ctctaatagc cggttggacg cacatactgt gtgcatatct gatccaaaat taagttttga
     4081 tgcaatgacg atcgttggaa atctcaaccg agacaacgct caagcccttt ctaaatttat
     4141 gagtgtagag ccccaaataa gactttggga tattcttcaa acaaagttta aagctaaagc
     4201 acttcaagaa aaagtttata ttgaatatga caaagtgaaa gcagatagtt gggatagacg
     4261 taatatgcgt attgaattta atccaaacaa acttacacga gatgaaatga tttggttaaa
     4321 acaaaatata ataagctaca tggaagatga cggttttaca agattagatt tagcctttga
     4381 ttttgaagat gatttgagtg actactatgc aatgtctgat aaagcagtta agaaaactat
     4441 tttttatggt cgtaatggta agccagaaac aaaatatttt ggcgtgagag atagtaatag
     4501 atttattaga atttataata aaaagcaaga acgtaaagat aatgcagatg ctgaagttat
     4561 gtctgaacat ttatggcgtg tagaaatcga acttaaaaga gatatggtgg attactggaa
     4621 tgattgcttt agtgatttac atatcttgca accagattgg aaaactatcc aacgcactgc
     4681 ggatagagca atagttttta tgttattgag tgatgaagaa gaatggggaa agcttcacag
     4741 aaattctaga acaaaatata agaatttgat aaaagaaatt tcgccagtcg atttaacgga
     4801 cttaatgaaa tcgactttaa aagcgaacga aaaacaattg caaaaacaaa tcgatttttg
     4861 gcaacatgaa tttaaatttt ggaaatagtg tacatattaa tattactgaa caaaaatgat
     4921 atatttaaac tattctaatt taggaggatt tttttatgaa gtgtctattt aaaaatttgg
     4981 ggaatttata tgaggtgaaa gaataattta cccctataaa ctttagtcac ctcaagtaaa
     5041 gaggtaaaat tgtttagttt atataaaaaa tttaaaggtt tgttttatag cgttttattt
     5101 tggctttgta ttctttcatt ttttagtgta ttaaatgaaa tggttttaaa tgtttcttta
     5161 cctgatattg caaatcattt taatactact cctggaatta caaactgggt aaacactgca
     5221 tatatgttaa ctttttcgat aggaacagca gtatatggaa aattatctga ttatataaat
     5281 ataaaaaaat tgttaattat tggtattagt ttgagctgtc ttggttcatt gattgctttt
     5341 attgggccca cctaggtatt atcaagataa gaaagaaaag gatttttcgc tacgctcaaa
     5401 tcctttaaaa aaacacaaaa gaccacattt tttaatgtgg tcttttattc ttcaactaaa
     5461 gcacccatta gttcaacaaa cgaaaattgg ataaagtggg atatttttaa aatatatatt
     5521 tatgttacag taatattgac ttttaaaaaa ggattgattc taatgaagaa agcagacaag
     5581 taagcctcct aaattcactt tagataaaaa tttaggaggc atatcaaatg aactttaata
     5641 aaattgattt agacaattgg aagagaaaag agatatttaa tcattatttg aaccaacaaa
     5701 cgacttttag tataaccaca gaaattgata ttagtgtttt ataccgaaac ataaaacaag
     5761 aaggatataa attttaccct gcatttattt tcttagtgac aagggtgata aactcaaata
     5821 cagcttttag aactggttac aatagcgacg gagagttagg ttattgggat aagttagagc
     5881 cactttatac aatttttgat ggtgtatcta aaacattctc tggtatttgg actcctgtaa
     5941 agaatgactt caaagagttt tatgatttat acctttctga tgtagagaaa tataatggtt
     6001 cggggaaatt gtttcccaaa acacctatac ctgaaaatgc tttttctctt tctattattc
     6061 catggacttc atttactggg tttaacttaa atatcaataa taatagtaat taccttctac
     6121 ccattattac agcaggaaaa ttcattaata aaggtaattc aatatattta ccgctatctt
     6181 tacaggtaca tcattctgtt tgtgatggtt atcatgcagg attgtttatg aactctattc
     6241 aggaattgtc agataggcct aatgactggc ttttataata tgagataatg ccgactgtac
     6301 tttttacagt cggttttcta atgtcactaa cctgccccgt tagttgaaga aggccgcggc
     6361 ctcgagcggc cgcatag
//
