LOCUS       sequence-456056-       10023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance.
            For use with AID degron..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, 
            Wan J, Fang D, Bintu L
  TITLE     The H3.3 K36M oncohistone disrupts the establishment of epigenetic 
            memory through loss of DNA methylation.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147.
  PUBMED    37873347
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 
            10.1101/2023.10.13.562147."
COMMENT     Sequence Label: pLenti-osTIR1-9xMyc-Zeo
FEATURES             Location/Qualifiers
     source          1..10023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             651..831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1340..1356
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1341..1357
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1360..1948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1466..1477
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1875..1895
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    2004..2037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2063..2437)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2456..2503)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2551..2880)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2565..2700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2619..2638)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2865..2885
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    2918..3125
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3114..3131
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3162..3191)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3198..3227)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3234..3263)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3291..5015)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     intron          5026..5964
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5049..5069)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(5965..6176)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    6299..6416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6714..6758)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6943..7176
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7673..7798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7845..8025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8043..8241)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8068..8088)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8243..8622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8794..8813
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8888..8992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8993..9061,9062..9853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9211..9230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg
     1381 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     1441 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     1501 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     1561 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     1621 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     1681 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     1741 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     1801 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     1861 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     1921 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
     1981 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
     2041 gtccctcgac gaattcgtcg actcagtcct gctcctcggc cacgaagtgc acgcagttgc
     2101 cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc tcggtcatgg
     2161 ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg gcgtacagct
     2221 cgtccaggcc gcgcacccac acccaggcca gggtgttgtc cggcaccacc tggtcctgga
     2281 ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga
     2341 agtcccggga gaacccgagc cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg
     2401 cggtgagcac cggaacggca ctggtcaact tggccatggt ttagttcctc accttgtcgt
     2461 attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct gatcagatcc
     2521 gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc
     2581 tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa
     2641 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     2701 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     2761 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     2821 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     2881 attccacaga attaattcgc gttaaattgc ggccgcgtcc ccagcatgcc tgctattgtc
     2941 ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct
     3001 actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc
     3061 ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg
     3121 cacagtcgag gccgatttaa attttagcta gtggatccgt tcaagtcttc ttctgagatt
     3181 aatttttgtt caccgttcaa gtcttcctcg gagattagct tttgttcacc gttcaaatct
     3241 tcttcagaaa tcaacttttg ttcaccacta gcagcagaac cggagtcgac taggatttta
     3301 acaaaatttg gtgcatcatc cctcgcccca gcagttgtgc ggtacacata taatttctcc
     3361 actttaggca gatctccatg gttttcctcc atttcattgc taccatcccg ctcatttatg
     3421 acctcaacat tgagcatcgg catctttgac gcaaggactt ggcacccctt taacgtgaca
     3481 ttgcaagatg acatccaaag ggatcgcatt gtctcgtacc tagcaaaatt ccccaagagt
     3541 gcagcatcac caaacgggct atctcttatc tccagtttcc ttaaattctt gcatccattc
     3601 ataacatgca tcataccctt atcactgtca ccagcaaatg ctatagaaag catctcaagt
     3661 tgttttgcat atttcccaat atacatgaaa actttgtctg tgagaagacc agatattgac
     3721 aaacgttgta atcccttgca ctcacgaaca atagctccaa agccttcatc taatggttgg
     3781 cttgtcacaa catcaggctt ccctggctca agaatacaaa gtctgaatcg tgtgaaattt
     3841 ggacagttct tggcgacagt aactagtgca gcattagtca tttggtgaca gaagtacagt
     3901 agtgagttca gttttggaca gcccaaggat actgcaacaa gtccctcctc tgtcactgca
     3961 gaataaccag ctacgtagaa atctgatgga aataccctga gttcttgcaa gtctttgcaa
     4021 ctggaggcca ccacttgcaa gcctttgtcc gagatacaat ccagtaccca aaggcgttgg
     4081 agcttcacac agcggctaat catttttgta aggtcagaag catcaagtgt gggtgcatag
     4141 ctcaagttca atcctgtcag ttgagcacac agggggtaga taaatgacag gcaaacagga
     4201 gaagcatccc agaatccaga caaactcctc aacatcttgc atttctccag agcactggta
     4261 agcttaaagt aggactcagt ttggaagtca tctgtcaaat tccctgtccc caaatcctcc
     4321 aagttagggg tacgcagtag tatctttgca agtgtatcta ccgatacaga tcgattcagc
     4381 ctcaaactcc gcaggtttgg ggacctgcta acaagtctct ccagtgaacc agcattaacc
     4441 tcccctttga tgcaggcaaa attcaatgag acaagtgatg tgcaggaatc agggaagcag
     4501 gaaagccacc taggccctcg atcctccact tcattttcct gcaaatccaa ctccctcaga
     4561 agcttgcaat ggctcgcgac ggcggctagc ccgtcagtgc tgaacccctc gcagctgata
     4621 agaacaagag ccctgaaccg cgggaacgag cgagccagca gctcgaggct ctcgtcggac
     4681 accaccatcc gcttcatcct gagctcctcc aggccgtggc atcccctcgc ggccgcctcg
     4741 atccacggcc ccgcgtagcc gccccagtcg gggggcacga ggttgaagtc ggcgaagtgg
     4801 ggcttcccct tcaccgtgag cgcccgcacg ttggggaacc gcgcggcgac gcggccggcg
     4861 cgcacggcgt agcagttgcc cacgaagacg ccgcggcggc tcagcctctc gatctcgtac
     4921 cacaccttgc agacgagcga gaccgtgttg cggtcgcgct gcgccggcag gaagctgaag
     4981 atgtgctcca ccacctcctc cgggaagtac gtcatctcac gacacctgaa atggaagaaa
     5041 aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc
     5101 aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc
     5161 agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca
     5221 aaagcgcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga
     5281 agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc
     5341 tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct
     5401 ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca
     5461 cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat
     5521 tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc
     5581 cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt ggcccgcatt
     5641 tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc
     5701 tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc gcacgcggcg
     5761 gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc cttaagcgca
     5821 aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa tcacgtactg
     5881 cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga ggccaggccc
     5941 gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc gaaaaagaac
     6001 gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag
     6061 ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca
     6121 attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg
     6181 acgggcacat gcatggcggt aatacggtta tccacgcggc cgcctgagtc aaatgggatc
     6241 tctagagcgg gcccggtact aaccaaactg gatctctgct gtccctgtaa taaacccgaa
     6301 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
     6361 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt
     6421 ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat
     6481 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
     6541 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     6601 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     6661 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     6721 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     6781 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     6841 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     6901 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     6961 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     7021 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     7081 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     7141 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     7201 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     7261 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     7321 atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     7381 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     7441 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     7501 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     7561 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     7621 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     7681 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     7741 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     7801 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     7861 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     7921 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     7981 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaacg
     8041 cgctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     8101 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     8161 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     8221 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     8281 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     8341 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     8401 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     8461 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     8521 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     8581 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
     8641 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
     8701 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
     8761 cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag
     8821 tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     8881 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8941 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9001 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9061 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9841 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10021 ttc
//
