LOCUS       sequence-456063-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the expression of the passenger domain of the Orientia 
            tsutsugamushi protein ScaA in HeLa Flp-In cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Manigrasso G, Saharat K, Kullapanich C, Boulanger J, Morgan TE, 
            Kramer H, Salje J, Carter AP
  TITLE     The intracellular bacterium Orientia tsutsugamushi hijacks the 
            adaptor protein BICD2 for dynein-based motility
  JOURNAL   bioRxiv 2024.04.11.589026
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.11.589026"
COMMENT     Sequence Label: pcDNA5/FRT/TO_eGFP-pScaA
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(987..989,990..992,993..1703)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1032..1053)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5401..5418)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5407..5631
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5764..5783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5915..5962
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5970..6992
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5970..6992
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7122..7243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7159..7178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7213..7232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7292..7308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7292..7308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7305..7327)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7316..7332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7340..7346,7347..7364,7365..7370))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7385..7406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7523..7540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7694..8282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7774..7793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8453..9244,9245..9313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9076..9095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9314..9418)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1021 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1081 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1141 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     1201 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1261 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1321 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1381 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1441 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1501 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1561 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1621 ccaacgagaa gcgcgatcac atggtcctta aggagttcgt gaccgccgcc gggatcactc
     1681 tcggcatgga cgagctgtac aagggaggtt caggtggatc tggaggtatg caaaccgctg
     1741 acaatgaccc ttctgcctct tcaagtagtt ctggagtgtt tactgggtca ggtgcatcac
     1801 agtccaacaa cgctaccgag tcctcaaatg acaatcaagg agatacaggg tcaggagcta
     1861 ccaacaaccc ttccaatccc atcgaaggag cttcaggcac cagtcctgca actggagcta
     1921 gcaatgctac cggtgattcc gacgccacag gcaacaagga agcagatggc gacgactcag
     1981 ttaccgactc tggagcaagt cagtccaaca atgctacaaa gggatcaaat gatgaccagg
     2041 gtgacaccag cggtggcaca gccagcaacc catcaaaccc tagtaaccca attgaaggag
     2101 ccagtggcac aagtcctgcc accggagctt cagatgccac cggagtcact gagactactg
     2161 gtggaagtgc tactggtgga gaggtgacag acggcaagaa gcccaagaag tcacctttca
     2221 agaaactgaa ggagtttggc tctacactct ccctgtcagg gaacactaaa ccagaggagc
     2281 cctcagaggg agctacaggc gagaaaggta gctctacttc aaccttcaag cggattcgca
     2341 agcagctcgc tagctctacc ctgtctctgt ccagtacaac ccacgaggaa tccaagccac
     2401 caatcgtcct gtcagatccc accaacaaga gcgttgagat caatcccaac aagggctctg
     2461 gcgttcagaa agttatcgtt aagcaacctg aagtcgcctt tgagaagatg gaagggaaga
     2521 acgatagcat cgagatcaac gttgagacag ataccaaagt gaccgtgaag ggtgacgacg
     2581 gcagtaagat caaactggtg aatctgaatc tgagtaactc agagctcatc atcaacaaca
     2641 caacttactc caactctgat ctcaagccaa cttctccaat cactagcagc gtctccatgc
     2701 ccaacctgtc cgatactgac gtcagctcct ctagctcacc acccacacgg aggtctagta
     2761 gtatcatcca acctacaggt ccacagccat ttgacctgtc cttcttctcc agcacaactc
     2821 acggtggtaa gaccgaagcc accgacagaa gtatcttcac cggtttcggg tcctttgtga
     2881 cacgcagccc aacaaccctg agctccgacc tcaagatatg tggcaacatc gacgttgaca
     2941 aaccactgca gctggttact gtgacaccag tgatgctcaa gactctgacc gttgaaagat
     3001 ccaaactgat tcccaagaag agcggtgtcc tcaagctgaa gagtaacctg atggccaaca
     3061 tcgaaggaga aggagctacc atcatcacca gcggttccag cttctccatc tccttcaaca
     3121 agttcggtac ccagaacaat ccaattcccg tgcaggtgga cggagtgctg gagctgcaag
     3181 ccgaactccc tcccagtgat tccaatgaag tcaacatgca tgcctcaatc acatctaaga
     3241 agcagtccgt cagtgaagtt catttcctcc actctcccaa ttccaatctc actcggtaca
     3301 acgtccacgg ctgcatcgga actccagatt ctattgtgga gaaggtggtg ctcaacggtc
     3361 ccacaatgct gaacgtgacc ggaaatgtgt atgtggataa gatgaaactg aaatcctcta
     3421 atcgggtgtc acatggcgat tccgagcctc acggagggat cgtgctgaac ggagaaatgt
     3481 attccagact cgttgagccc agtatcccaa actacggcaa gatcactatt catggtaatc
     3541 catccaccaa gtacgacagt aacaactaca agtcaatctt ctacgggaac atcggtaccg
     3601 ataagaacaa ggtcagtcgg atagagtttg ccgagcatct gaacaaccct aacgggatca
     3661 ggcctcacac tgagctcttt gtctcatatg acaaatcaac taacaagagt accatcttct
     3721 gtagcgacat catcttctcc catccagaag atactgtgaa cattctgggt aacactgaca
     3781 tcaaaggaaa cattggcacc aaaggtaact ctggaggtag gatcatcgtg cgcaacaacg
     3841 ggaccctgac ccacagcgga ggcgacatag ggaacaacaa gacttccggt agcaacctgc
     3901 tgcaggccat ccacctgtgt gagggagcat ccttcatttc aaagcatgat agtaacatca
     3961 tccctcacac ctgcaacatc aaggagatta cactggataa gaactccaac atcgagctga
     4021 acagtaatgc cgactggaag accgatatca ttcctaaggg cactggtgca aagctcacaa
     4081 tgagccaagg gtcatactcc ggaaacatag ggaaccaggc taatcccatg gaaagtgtga
     4141 cactgctgcc taagagtaag aggccttccc tcatcgaggt ggaaggctcc gtgtatactc
     4201 accagtgcag cataagcgat aagtcaagtg ccaagggaga agtgaaactg ctgggagact
     4261 ataccataac agctcaaact aacgaagaga atggtggaat ctccattggg aacgccaaga
     4321 tcgacctctc atcaaacacc ctgaccctgg cctccgatag cataagcatc ctgggtcata
     4381 agtctaagaa gggctcctca acaaatcccg agaagttcaa gaagaagcct gtgactgtct
     4441 tcagcatcaa ctacactact aacagtgatg gcaccgtgaa gacccaaggt aaactgaagc
     4501 tgtctaagta ctacgatccc aacaacaaga tcatcgtcaa cgtcgatcat caaggcgacc
     4561 tcagggatct ctcaaagcac ggaacccaaa ctgtcattca accagtggac atcgccaata
     4621 caggaggact cgctgtggac aatctggtgt acatctgtga tggcaagtgt aagtggcagc
     4681 cagtggagtg ctcagacggg aagttcgtgt ttgctgggct ggtcgataag aagaagaaag
     4741 ataagggaga tgacgggacc agtggctcct caggctcctc tgctgctcct tctgccaggg
     4801 tgtcacctga agtctctgat gatgaggaag gcgacaaagg agaggacatt caggcatcaa
     4861 cagtctcaca cctgcactct gaggatagtg cccatggcaa gcagtcccat gggaaagacc
     4921 acaccgagga cccaaaggac cctaccgaag atgccattga ggctctggcc aggctgctgg
     4981 acgatccagc accacctcca caaccagctg acgaacaacc tcaacctgac ccagatgaac
     5041 acgtgcagcc tgtcgtggca cagcccatcg ctgctggtgc tggccagcag cagcagcaac
     5101 aacaacagca acaacagcaa cagcagcagc agcaacagca acaacagcag caacagcaga
     5161 gatacgtcta cacatccagc ccttcatccg atgaccagga gcagagcagc aaacctgaac
     5221 ccatcctgca gccaattctc ccaaagatca gtgacgctca tctgaagtca ggcgttcacg
     5281 caaccgtggc tgccatcagt agcatcgttg agtctaaagc tgctaacaac aattctggct
     5341 cagacatcgt gagttcaggg tctaacatca acaaacagtg ataaagcggc cgctgatcag
     5401 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5461 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5521 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5581 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5641 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5701 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5761 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5821 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5881 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
     5941 ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg
     6001 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     6061 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     6121 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     6181 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     6241 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     6301 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     6361 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     6421 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     6481 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     6541 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     6601 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     6661 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     6721 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     6781 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     6841 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     6901 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     6961 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
     7021 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7081 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     7141 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7201 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7261 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7321 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7381 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7441 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7501 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8461 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8521 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8581 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8641 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8701 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8761 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8821 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8881 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8941 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9001 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9061 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9121 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9181 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9241 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9301 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9361 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9421 catttccccg aaaagtgcca cctgacgtc
//
