LOCUS       sequence-456066-        2192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 promoter driving expression of zntR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   McNerney MP, Zhang Y, Steppe P, Silverman AD, Jewett MC, Styczynski 
            MP
  TITLE     Point-of-care biomarker quantification enabled by sample-specific 
            calibration.
  JOURNAL   Sci Adv. 2019 Sep 25;5(9):eaax4473. doi: 10.1126/sciadv.aax4473. 
            eCollection 2019 Sep.
  PUBMED    31579825
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-09-25"; volume: "5(9):eaax4473. doi"; pages: " 
            10.1126/sciadv.aax4473. eCollection 2019 Sep"
COMMENT     Sequence Label: pT7_zntR
FEATURES             Location/Qualifiers
     source          1..2192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             683..705
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1193..1211)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1207..1254
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1272..2087)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1995..2014
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2079..2098
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatgtatcg
      721 cattggtgag ctggcaaaaa tggcggaagt aacacccgac acgattcgtt attacgaaaa
      781 acagcagatg atggagcatg aagtgcgtac tgaaggtggg tttcgcctat ataccgaaag
      841 cgatctccag cgattgaaat ttatccgcca tgccagacaa ctaggtttca gtctggagtc
      901 gatccgcgag ttgctgtcga tccgcatcga tcctgaacac catacctgtc aggagtcaaa
      961 aggcattgtg caggaaagat tgcaggaagt cgaagcacgg atagccgagt tgcagagtat
     1021 gcagcgttcc ttgcaacgcc ttaacgatgc ctgttgtggg actgctcata gcagtgttta
     1081 ttgttcgatt cttgaagctc ttgaacaagg ggcgagtggc gttaagagtg gttgttgata
     1141 agtcgaccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     1201 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     1261 gccaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1321 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1381 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     1441 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     1501 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     1561 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     1621 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     1681 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     1741 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     1801 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     1861 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     1921 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     1981 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2041 tttaatcgcg gcttcgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     2101 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     2161 atgtaacatc agagattttg agacacaacg tg
//
