LOCUS       sequence-456073-        5143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, 
            and ulaC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Piorino F, Styczynski MP
  TITLE     Harnessing Escherichia coli's Native Machinery for Detection of 
            Vitamin C (Ascorbate) Deficiency.
  JOURNAL   ACS Synth Biol. 2022 Nov 18;11(11):3592-3600. doi: 
            10.1021/acssynbio.2c00335. Epub 2022 Oct 27.
  PUBMED    36300901
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00335"; journalName: "ACS Synth Biol"; date: 
            "2022-11-18- 18"; volume: "11"; issue: "11"; pages: "3592-3600"
COMMENT     Sequence Label: pBad_UlaABC
FEATURES             Location/Qualifiers
     source          1..5143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(617..1495)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1522..1806
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1729..1748
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1854..1876
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      3922..3931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4128..4146)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4142..4189
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4207..5022)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4930..4949
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5014..5033
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgatcccg cgaaatttat gacaacttga cggctacatc attcactttt tcttcacaac
      661 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga
      721 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca
      781 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta
      841 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc
      901 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
      961 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
     1021 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
     1081 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
     1141 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
     1201 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
     1261 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat
     1321 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
     1381 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
     1441 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt
     1501 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
     1561 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca
     1621 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
     1681 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat
     1741 ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt
     1801 ctccataccc gtttttttgg gctagcccac aacggtttcc ctctagaaat aattttgttt
     1861 aactttaaga aggagatata catatggaga tcctctacaa catctttacc gtgtttttta
     1921 accaggtcat gaccaatgcc ccgttgttgc tgggtattgt gacctgtctg ggctacatcc
     1981 tactgcgcaa aagtgtcagc gttattatta aaggcacgat taaaaccata attggtttca
     2041 tgttgttgca ggcagggtcc ggcatcctca ccagcacctt caaaccggtg gtggcgaaaa
     2101 tgtccgaagt ctacggcatt aacggcgcaa tttccgatac ctacgcttca atgatggcaa
     2161 ccatcgaccg catgggcgat gcctatagct gggtgggtta cgccgtattg ttagcgctgg
     2221 cgctgaacat ctgttacgtg ctgttgcgtc gcattaccgg cattcgcaca atcatgttga
     2281 ccggccacat catgttccag caggccgggt tgattgccgt tacgctgttt atcttcggct
     2341 actccatgtg gaccaccatt atctgtaccg cgattctggt ttcgctctac tggggcatca
     2401 cttccaacat gatgtacaag ccgactcagg aagtgacgga tggctgtggt ttctccatcg
     2461 gtcaccagca gcagtttgca tcatggattg cctataaagt cgcgccgttc ctcggcaaaa
     2521 aagaggagag cgttgaagac ctcaaattgc cgggctggct gaacattttc cacgacaaca
     2581 tcgtctccac ggcgattgtg atgaccatct tctttggtgc cattctgctc tccttcggta
     2641 tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtgtacatc ctgcaaactg
     2701 gtttctcctt tgcggtggcg atcttcatca tcacgcaggg tgtgcgcatg tttgtggcgg
     2761 aactctctga agcatttaac ggcatttccc agcgcctgat cccaggtgcg gttctggcga
     2821 ttgactgtgc agctatctat agcttcgcgc cgaacgccgt ggtctggggc tttatgtggg
     2881 gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggctcc tcgatcctga
     2941 ttattcctgg ctttatcccg atgttcttct ctaacgccac catcggcgtg ttcgctaacc
     3001 acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg atcgaaatct
     3061 ttggttgcgt ctgggcggtg aaactcaccg gtatgagtgc ctggatgggc atggcggact
     3121 ggtcgattct ggcaccgccg atgatgcaag gcttcttctc catcggtatc gcctttatgg
     3181 ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgcg ctgcgcgcag
     3241 aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt ttgattatga
     3301 ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc atgaagatga
     3361 aagtggacca gtttttaacc caatcaaaca ttgaccatac ggtaaacagc tgcgcggttg
     3421 gcgagtacaa aagcgagttg agtggcgcgg atatcatcat cgcttctacg cacattgcgg
     3481 gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg ctctctcctg
     3541 ccgactttgg cccgaaactg ctggaagtga tcaaagagca tttcccgcag gatgtgaagt
     3601 aaggacgcgc catgaaatta cgtgattcgc tggcggaaaa taaatccatc cgcctgcagg
     3661 ctgaagcaga gacatggcag gaagcggtga aaatcggcgt tgacctgctg gtggcggcag
     3721 atgtggtaga gccgcgttac taccaggcga ttctggatgg cgttgaacag ttcggtcctt
     3781 atttcgttat cgctccgggc ctggcaatgc cgcacgggcg tccggaagag ggcgttaaga
     3841 aaaccggttt ctctctggta acgctgaaaa agccgctgga gttcaaccac gatgacaacg
     3901 atccggtgga tatcctcatc accatggcgg cggtcgatgc caatactcac caggaagtgg
     3961 gcatcatgca gatcgtcaac ctgtttgaag atgaagagaa ttttgaccgt ttacgcgcct
     4021 gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca gcttaagtcg
     4081 accggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     4141 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaagccaa
     4201 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     4261 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     4321 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     4381 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     4441 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
     4501 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     4561 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     4621 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     4681 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     4741 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     4801 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     4861 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     4921 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     4981 tcgcggcttc gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     5041 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     5101 acatcagaga ttttgagaca caacgtgaga tcaaaggatc ttc
//
