LOCUS       sequence-456085-        5419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of BICD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Manigrasso G, Saharat K, Kullapanich C, Boulanger J, Morgan TE, 
            Kramer H, Salje J, Carter AP
  TITLE     The intracellular bacterium Orientia tsutsugamushi hijacks the 
            adaptor protein BICD2 for dynein-based motility
  JOURNAL   bioRxiv 2024.04.11.589026
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.11.589026"
COMMENT     Sequence Label: pACEBac1_Strep-Psc-BICD2
FEATURES             Location/Qualifiers
     source          1..5419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             320..343
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             380..403
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             404..427
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             913..924
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3041..3175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3078..3097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3132..3151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3296..3461
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3651..4239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4140..4159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4374..4597
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4664..5197)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5386..5391,5392..5408,5409..5414))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcggatccc tcgagcatgt ggtcacatcc gcagtttgaa aaaggtagcg caggtagtgc
      361 agcaggtagc ggtgcaggtt ggagccatcc tcagtttgag aaactggaag ttctgtttca
      421 gggtccgatg tcagctccat cagaagaaga ggaatatgct aggttggtca tggaagccca
      481 gcccgagtgg ctcagggctg aagttaagcg cctgtctcac gagctcgccg agacaaccag
      541 agagaagatc caagcagctg aatatggcct cgctgttttg gaggagaaac accaactgaa
      601 actccagttc gaggagctgg aggttgacta cgaggccatt aggtcagaaa tggagcaact
      661 gaaagaagcc ttcggccaag cacataccaa tcataagaag gttgccgcag acggagagtc
      721 cagagaagaa tcactgatcc aggagtctgc tagcaaggag cagtattacg tgaggaaagt
      781 cctggagctg cagaccgagt tgaagcaact caggaacgtt ctgaccaaca cccaatctga
      841 aaacgagaga ttgacaagcg tggcccaaga gctcaaggaa atcaatcaga acgtcgagat
      901 tcaacgcggt agattgaggg acgacatcaa agaatacaaa ttcagggagg caagactcct
      961 ccaagactac tcagagctgg aagaggagaa catctctctg cagaaacaag tctcagtgct
     1021 caggcaaaat caggtggagt tcgaaggact gaagcacgaa atcaagcgcc tcgaagagga
     1081 gacagagtac ctgaactccc aactggagga cgctatcaga ctgaaggaga tttcagaaag
     1141 acagctcgaa gaagccctgg aaacactgaa gacagaaagg gagcagaaga ataacctccg
     1201 caaggagttg agccactata tgtctatcaa cgattccttc tacacttcac atctccaggt
     1261 cagcttggac ggactgaagt tcagcgacga cactgttacc gccgagccca acaatgatgc
     1321 cgaagctctc gtgaacggat tcgaacatag cggcctggtc aaatcctcat tggataacaa
     1381 gactagcact ccacgcaagg atggtctggc tcctccctct ccttccttgg tgtccgatct
     1441 gttgagcgag ttgcacatca gcgaaatcca gaagctcaag cagcagctcg tccaaatgga
     1501 gcgcgagaag gtgggtctgc tggccacatt gcaagatact cagaaacagt tggagcaggc
     1561 tagaggtacc ttgtcagagc agcatgagaa ggtgaacagg ttgaccgaga atctgagcgc
     1621 tctgaggcgc ctccaggctg gtaaggagcg ccaaacttct ttggacaatg agaaagatcg
     1681 cgattctcac gaggacggag actactacga agttgatatc aatggacccg agatcctcgc
     1741 atgcaaatac catgtcgctg tggctgaggc cggcgagttg cgcgagcagt tgaaggcttt
     1801 gcgctctacc cacgaggcca gagaggcaca gcacgctgag gagaaaggac gctatgaggc
     1861 agaaggtcag gccctgacag agaagatcag cctcctggag aaagcctcac accaggatag
     1921 ggagctgctc gcacatctcg aaaaggagct caagaaggtg tctgatgtcg ccggagagac
     1981 acagggctcc ttgaatgtcg ctcaggacga gctcgtgacc ttctccgagg aactcgctaa
     2041 cctgtaccat cacgtgtgca tgtgcaacaa cgagactccc aacagggtca tgctggacta
     2101 ttatcgcgaa ggacagggca aagccggacg cactagccca gagggtaggg gaaggagatc
     2161 acctgttttg ctccctaagg gtctgctggc aacagaagtt ggtagggccg atggcggcac
     2221 aggagataat tcaccaagcc cctccagctc tctgccatcc ccattgtccg accccagacg
     2281 cgagccaatg aatatctata acttgatcgc tattatcaga gatcagatta agcacctgca
     2341 agccgctgtt gaccgcacca ccgagctctc tcgccaaaga attgcttctc aagagttggg
     2401 tccagccgtt gataaggaca aggaagctct catggaggaa atcctgaaac tcaagtctct
     2461 gctgtccacc aagagggagc agattaccac cctgcgcact gtcctgaagg ctaacaaaca
     2521 gacagccgag gtcgctctcg ctaacttgaa gtccaaatat gagaacgaga aggctatggt
     2581 cacagaaacc atgatgaaat tgcgcaatga gctcaaggct ctcaaggaag acgctgctac
     2641 ttttagcagc ctgagggcaa tgttcgccac aagatgcgac gaatatatca cccagctcga
     2701 cgaaatgcag agacagctcg ctgctgctga agacgagaag aaaactctca actcattgct
     2761 gaggatggca attcagcaaa agctggctct gacacagcgc ctggaactgc tggagctgga
     2821 tcatgaacaa accaggcgcg gtcgctccaa agctgccagc aaggccaagc ctgcatctcc
     2881 ttctctgtaa tctagagcct gcagtctcga caagcttgtc gagaagtact agaggatcat
     2941 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3001 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3061 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3121 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc
     3181 actgcttgag cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     3241 ctgccaccgc tgagcaataa ctatcataac ccctagggta tacccatcta attggaacca
     3301 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
     3361 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
     3421 tttccacccc tcccagttcc caactatttt gtccgcccac aaccggttga cttgggtcaa
     3481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4321 gattgacttg ggtcgctctt cctgtggatg cgcagatgcc ctgcgtaagc gggtgtgggc
     4381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
     4441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
     4501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
     4561 agaggggcgt ggccaagggc atgtaaagac tatattcgcg gcgttgtgac aatttaccga
     4621 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     4681 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     4741 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     4801 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     4861 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     4921 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     4981 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     5041 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     5101 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     5161 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5221 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     5281 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     5341 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     5401 caggcttatg tcaactggg
//
