LOCUS       sequence-456089-        3232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of the mutant C-terminus of BICD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Manigrasso G, Saharat K, Kullapanich C, Boulanger J, Morgan TE, 
            Kramer H, Salje J, Carter AP
  TITLE     The intracellular bacterium Orientia tsutsugamushi hijacks the 
            adaptor protein BICD2 for dynein-based motility
  JOURNAL   bioRxiv 2024.04.11.589026
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.11.589026"
COMMENT     Sequence Label: pACEBac1_Strep-Psc-BICD2(711-800)_L742A
FEATURES             Location/Qualifiers
     source          1..3232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             320..343
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             380..403
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             404..427
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    854..988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(891..910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     945..964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1109..1274
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1464..2052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1953..1972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2187..2410
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2477..3010)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3199..3204,3205..3221,3222..3227))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcggatccc tcgagcatgt ggtcacatcc gcagtttgaa aaaggtagcg caggtagtgc
      361 agcaggtagc ggtgcaggtt ggagccatcc tcagtttgag aaactggaag ttctgtttca
      421 gggtccgatg tatgagaacg agaaggctat ggtcacagaa accatgatga aattgcgcaa
      481 tgagctcaag gctctcaagg aagacgctgc tacttttagc agcgcaaggg caatgttcgc
      541 cacaagatgc gacgaatata tcacccagct cgacgaaatg cagagacagc tcgctgctgc
      601 tgaagacgag aagaaaactc tcaactcatt gctgaggatg gcaattcagc aaaagctggc
      661 tctgacacag cgcctggaac tgctggagct ggatcatgaa taatctagag cctgcagtct
      721 cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt
      781 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      841 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      961 catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg
     1021 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat
     1081 aacccctagg gtatacccat ctaattggaa ccagataagt gaaatctagt tccaaactat
     1141 tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg
     1201 tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat
     1261 tttgtccgcc cacaaccggt tgacttgggt caactgtcag accaagttta ctcatatata
     1321 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1381 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1441 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1501 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1561 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1621 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1681 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1741 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1801 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1861 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1921 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1981 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2041 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2101 tttgctcaca tgttctttcc tgcgttatcc cctgattgac ttgggtcgct cttcctgtgg
     2161 atgcgcagat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa
     2221 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
     2281 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
     2341 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatgtaaa
     2401 gactatattc gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct
     2461 cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt
     2521 cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca
     2581 cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg
     2641 tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac
     2701 tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt
     2761 ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg
     2821 agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa
     2881 gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca
     2941 tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     3001 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     3061 tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc
     3121 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     3181 atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg gg
//
