LOCUS       sequence-456093-        4339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of C-terminal portion of BICD2 (aa 342-800).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Manigrasso G, Saharat K, Kullapanich C, Boulanger J, Morgan TE, 
            Kramer H, Salje J, Carter AP
  TITLE     The intracellular bacterium Orientia tsutsugamushi hijacks the 
            adaptor protein BICD2 for dynein-based motility
  JOURNAL   bioRxiv 2024.04.11.589026
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.11.589026"
COMMENT     Sequence Label: pACEBac1_Strep-Psc-BICD2(342-800)
FEATURES             Location/Qualifiers
     source          1..4339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             320..343
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             380..403
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             404..427
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    1961..2095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1998..2017)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2052..2071
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2216..2381
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2571..3159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3060..3079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3294..3517
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3584..4117)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4306..4311,4312..4328,4329..4334))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcggatccc tcgagcatgt ggtcacatcc gcagtttgaa aaaggtagcg caggtagtgc
      361 agcaggtagc ggtgcaggtt ggagccatcc tcagtttgag aaactggaag ttctgtttca
      421 gggtccgatg ttgcacatca gcgaaatcca gaagctcaag cagcagctcg tccaaatgga
      481 gcgcgagaag gtgggtctgc tggccacatt gcaagatact cagaaacagt tggagcaggc
      541 tagaggtacc ttgtcagagc agcatgagaa ggtgaacagg ttgaccgaga atctgagcgc
      601 tctgaggcgc ctccaggctg gtaaggagcg ccaaacttct ttggacaatg agaaagatcg
      661 cgattctcac gaggacggag actactacga agttgatatc aatggacccg agatcctcgc
      721 atgcaaatac catgtcgctg tggctgaggc cggcgagttg cgcgagcagt tgaaggcttt
      781 gcgctctacc cacgaggcca gagaggcaca gcacgctgag gagaaaggac gctatgaggc
      841 agaaggtcag gccctgacag agaagatcag cctcctggag aaagcctcac accaggatag
      901 ggagctgctc gcacatctcg aaaaggagct caagaaggtg tctgatgtcg ccggagagac
      961 acagggctcc ttgaatgtcg ctcaggacga gctcgtgacc ttctccgagg aactcgctaa
     1021 cctgtaccat cacgtgtgca tgtgcaacaa cgagactccc aacagggtca tgctggacta
     1081 ttatcgcgaa ggacagggca aagccggacg cactagccca gagggtaggg gaaggagatc
     1141 acctgttttg ctccctaagg gtctgctggc aacagaagtt ggtagggccg atggcggcac
     1201 aggagataat tcaccaagcc cctccagctc tctgccatcc ccattgtccg accccagacg
     1261 cgagccaatg aatatctata acttgatcgc tattatcaga gatcagatta agcacctgca
     1321 agccgctgtt gaccgcacca ccgagctctc tcgccaaaga attgcttctc aagagttggg
     1381 tccagccgtt gataaggaca aggaagctct catggaggaa atcctgaaac tcaagtctct
     1441 gctgtccacc aagagggagc agattaccac cctgcgcact gtcctgaagg ctaacaaaca
     1501 gacagccgag gtcgctctcg ctaacttgaa gtccaaatat gagaacgaga aggctatggt
     1561 cacagaaacc atgatgaaat tgcgcaatga gctcaaggct ctcaaggaag acgctgctac
     1621 ttttagcagc ctgagggcaa tgttcgccac aagatgcgac gaatatatca cccagctcga
     1681 cgaaatgcag agacagctcg ctgctgctga agacgagaag aaaactctca actcattgct
     1741 gaggatggca attcagcaaa agctggctct gacacagcgc ctggaactgc tggagctgga
     1801 tcatgaataa tctagagcct gcagtctcga caagcttgtc gagaagtact agaggatcat
     1861 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     1921 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     1981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc
     2101 actgcttgag cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     2161 ctgccaccgc tgagcaataa ctatcataac ccctagggta tacccatcta attggaacca
     2221 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
     2281 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
     2341 tttccacccc tcccagttcc caactatttt gtccgcccac aaccggttga cttgggtcaa
     2401 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2461 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2521 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2581 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2641 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2701 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2761 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2821 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2881 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2941 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3001 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3061 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3121 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3181 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3241 gattgacttg ggtcgctctt cctgtggatg cgcagatgcc ctgcgtaagc gggtgtgggc
     3301 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
     3361 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
     3421 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
     3481 agaggggcgt ggccaagggc atgtaaagac tatattcgcg gcgttgtgac aatttaccga
     3541 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     3601 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     3661 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     3721 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     3781 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     3841 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     3901 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     3961 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     4021 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     4081 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     4141 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     4201 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     4261 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     4321 caggcttatg tcaactggg
//
