LOCUS       sequence-456096-        5089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of truncated BICD2 (aa 1-710).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Manigrasso G, Saharat K, Kullapanich C, Boulanger J, Morgan TE, 
            Kramer H, Salje J, Carter AP
  TITLE     The intracellular bacterium Orientia tsutsugamushi hijacks the 
            adaptor protein BICD2 for dynein-based motility
  JOURNAL   bioRxiv 2024.04.11.589026
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.11.589026"
COMMENT     Sequence Label: pACEBac1_Strep-Psc-BICD2(1-710)
FEATURES             Location/Qualifiers
     source          1..5089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             320..343
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             380..403
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             404..427
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             913..924
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2711..2845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2748..2767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2802..2821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2966..3131
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3321..3909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3810..3829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4044..4267
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4334..4867)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5056..5061,5062..5078,5079..5084))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcggatccc tcgagcatgt ggtcacatcc gcagtttgaa aaaggtagcg caggtagtgc
      361 agcaggtagc ggtgcaggtt ggagccatcc tcagtttgag aaactggaag ttctgtttca
      421 gggtccgatg tcagctccat cagaagaaga ggaatatgct aggttggtca tggaagccca
      481 gcccgagtgg ctcagggctg aagttaagcg cctgtctcac gagctcgccg agacaaccag
      541 agagaagatc caagcagctg aatatggcct cgctgttttg gaggagaaac accaactgaa
      601 actccagttc gaggagctgg aggttgacta cgaggccatt aggtcagaaa tggagcaact
      661 gaaagaagcc ttcggccaag cacataccaa tcataagaag gttgccgcag acggagagtc
      721 cagagaagaa tcactgatcc aggagtctgc tagcaaggag cagtattacg tgaggaaagt
      781 cctggagctg cagaccgagt tgaagcaact caggaacgtt ctgaccaaca cccaatctga
      841 aaacgagaga ttgacaagcg tggcccaaga gctcaaggaa atcaatcaga acgtcgagat
      901 tcaacgcggt agattgaggg acgacatcaa agaatacaaa ttcagggagg caagactcct
      961 ccaagactac tcagagctgg aagaggagaa catctctctg cagaaacaag tctcagtgct
     1021 caggcaaaat caggtggagt tcgaaggact gaagcacgaa atcaagcgcc tcgaagagga
     1081 gacagagtac ctgaactccc aactggagga cgctatcaga ctgaaggaga tttcagaaag
     1141 acagctcgaa gaagccctgg aaacactgaa gacagaaagg gagcagaaga ataacctccg
     1201 caaggagttg agccactata tgtctatcaa cgattccttc tacacttcac atctccaggt
     1261 cagcttggac ggactgaagt tcagcgacga cactgttacc gccgagccca acaatgatgc
     1321 cgaagctctc gtgaacggat tcgaacatag cggcctggtc aaatcctcat tggataacaa
     1381 gactagcact ccacgcaagg atggtctggc tcctccctct ccttccttgg tgtccgatct
     1441 gttgagcgag ttgcacatca gcgaaatcca gaagctcaag cagcagctcg tccaaatgga
     1501 gcgcgagaag gtgggtctgc tggccacatt gcaagatact cagaaacagt tggagcaggc
     1561 tagaggtacc ttgtcagagc agcatgagaa ggtgaacagg ttgaccgaga atctgagcgc
     1621 tctgaggcgc ctccaggctg gtaaggagcg ccaaacttct ttggacaatg agaaagatcg
     1681 cgattctcac gaggacggag actactacga agttgatatc aatggacccg agatcctcgc
     1741 atgcaaatac catgtcgctg tggctgaggc cggcgagttg cgcgagcagt tgaaggcttt
     1801 gcgctctacc cacgaggcca gagaggcaca gcacgctgag gagaaaggac gctatgaggc
     1861 agaaggtcag gccctgacag agaagatcag cctcctggag aaagcctcac accaggatag
     1921 ggagctgctc gcacatctcg aaaaggagct caagaaggtg tctgatgtcg ccggagagac
     1981 acagggctcc ttgaatgtcg ctcaggacga gctcgtgacc ttctccgagg aactcgctaa
     2041 cctgtaccat cacgtgtgca tgtgcaacaa cgagactccc aacagggtca tgctggacta
     2101 ttatcgcgaa ggacagggca aagccggacg cactagccca gagggtaggg gaaggagatc
     2161 acctgttttg ctccctaagg gtctgctggc aacagaagtt ggtagggccg atggcggcac
     2221 aggagataat tcaccaagcc cctccagctc tctgccatcc ccattgtccg accccagacg
     2281 cgagccaatg aatatctata acttgatcgc tattatcaga gatcagatta agcacctgca
     2341 agccgctgtt gaccgcacca ccgagctctc tcgccaaaga attgcttctc aagagttggg
     2401 tccagccgtt gataaggaca aggaagctct catggaggaa atcctgaaac tcaagtctct
     2461 gctgtccacc aagagggagc agattaccac cctgcgcact gtcctgaagg ctaacaaaca
     2521 gacagccgag gtcgctctcg ctaacttgaa gtccaaataa tctagagcct gcagtctcga
     2581 caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt
     2641 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     2701 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     2761 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     2821 caatgtatct tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta
     2881 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac
     2941 ccctagggta tacccatcta attggaacca gataagtgaa atctagttcc aaactatttt
     3001 gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca
     3061 ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt
     3121 gtccgcccac aaccggttga cttgggtcaa ctgtcagacc aagtttactc atatatactt
     3181 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3241 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3301 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3361 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3421 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3481 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3541 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3601 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3661 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3721 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3781 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3841 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3901 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3961 gctcacatgt tctttcctgc gttatcccct gattgacttg ggtcgctctt cctgtggatg
     4021 cgcagatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact gaacaaaata
     4081 gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac tgaaatcagt
     4141 ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa actcttcatt
     4201 ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc atgtaaagac
     4261 tatattcgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag ccgatctcgg
     4321 cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc
     4381 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt
     4441 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg
     4501 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac
     4561 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt
     4621 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt
     4681 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag
     4741 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac
     4801 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc
     4861 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     4921 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     4981 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     5041 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactggg
//
