LOCUS       sequence-45610-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V
  TITLE     A retro-lentiviral system for doxycycline-inducible gene expression 
            and gene knockdown in cells with limited proliferative capacity.
  JOURNAL   Mol Biol Rep. 2010 Apr . 37(4):1987-91.
  PUBMED    19655272
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep. 2010 Apr . 37(4):1987-91."
COMMENT     Sequence Label: pMA3160
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    67..184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(478..522)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tctagctaga gcgggccggc ccggtactaa ccaaactgga tctctgctgt ccctgtaata
       61 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
      121 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
      181 aaaattgtgg atgaatactg ccatttgtcg aattggcgca cgcagtgccg atccgttcac
      241 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
      301 gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct
      361 aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac
      421 tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa
      481 acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga
      541 ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat
      601 ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg
      661 cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc
      721 ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt
      781 gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcaa
      841 caagg
//
