LOCUS       sequence-456100-        4165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of N-terminal portion of BICD2 (aa 1-400).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Manigrasso G, Saharat K, Kullapanich C, Boulanger J, Morgan TE, 
            Kramer H, Salje J, Carter AP
  TITLE     The intracellular bacterium Orientia tsutsugamushi hijacks the 
            adaptor protein BICD2 for dynein-based motility
  JOURNAL   bioRxiv 2024.04.11.589026
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.11.589026"
COMMENT     Sequence Label: pACEBac1_Strep-Psc-BICD2(1-400)
FEATURES             Location/Qualifiers
     source          1..4165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             320..343
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             380..403
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             404..427
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             913..924
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    1787..1921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1824..1843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1878..1897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2042..2207
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2397..2985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2886..2905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3120..3343
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3410..3943)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4132..4137,4138..4154,4155..4160))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcggatccc tcgagcatgt ggtcacatcc gcagtttgaa aaaggtagcg caggtagtgc
      361 agcaggtagc ggtgcaggtt ggagccatcc tcagtttgag aaactggaag ttctgtttca
      421 gggtccgatg tcagctccat cagaagaaga ggaatatgct aggttggtca tggaagccca
      481 gcccgagtgg ctcagggctg aagttaagcg cctgtctcac gagctcgccg agacaaccag
      541 agagaagatc caagcagctg aatatggcct cgctgttttg gaggagaaac accaactgaa
      601 actccagttc gaggagctgg aggttgacta cgaggccatt aggtcagaaa tggagcaact
      661 gaaagaagcc ttcggccaag cacataccaa tcataagaag gttgccgcag acggagagtc
      721 cagagaagaa tcactgatcc aggagtctgc tagcaaggag cagtattacg tgaggaaagt
      781 cctggagctg cagaccgagt tgaagcaact caggaacgtt ctgaccaaca cccaatctga
      841 aaacgagaga ttgacaagcg tggcccaaga gctcaaggaa atcaatcaga acgtcgagat
      901 tcaacgcggt agattgaggg acgacatcaa agaatacaaa ttcagggagg caagactcct
      961 ccaagactac tcagagctgg aagaggagaa catctctctg cagaaacaag tctcagtgct
     1021 caggcaaaat caggtggagt tcgaaggact gaagcacgaa atcaagcgcc tcgaagagga
     1081 gacagagtac ctgaactccc aactggagga cgctatcaga ctgaaggaga tttcagaaag
     1141 acagctcgaa gaagccctgg aaacactgaa gacagaaagg gagcagaaga ataacctccg
     1201 caaggagttg agccactata tgtctatcaa cgattccttc tacacttcac atctccaggt
     1261 cagcttggac ggactgaagt tcagcgacga cactgttacc gccgagccca acaatgatgc
     1321 cgaagctctc gtgaacggat tcgaacatag cggcctggtc aaatcctcat tggataacaa
     1381 gactagcact ccacgcaagg atggtctggc tcctccctct ccttccttgg tgtccgatct
     1441 gttgagcgag ttgcacatca gcgaaatcca gaagctcaag cagcagctcg tccaaatgga
     1501 gcgcgagaag gtgggtctgc tggccacatt gcaagatact cagaaacagt tggagcaggc
     1561 tagaggtacc ttgtcagagc agcatgagaa ggtgaacagg ttgaccgaga atctgagcgc
     1621 tctgaggtaa ggtacctcta gagcctgcag tctcgacaag cttgtcgaga agtactagag
     1681 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     1741 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     1921 ctgatcactg cttgagccta gaagatccgg ctgctaacaa agcccgaaag gaagctgagt
     1981 tggctgctgc caccgctgag caataactat cataacccct agggtatacc catctaattg
     2041 gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc
     2101 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa
     2161 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaacc ggttgacttg
     2221 ggtcaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2281 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2341 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2401 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2461 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2521 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2581 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2641 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2701 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2761 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2821 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2881 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2941 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3001 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3061 tcccctgatt gacttgggtc gctcttcctg tggatgcgca gatgccctgc gtaagcgggt
     3121 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
     3181 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
     3241 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
     3301 tattaaagag gggcgtggcc aagggcatgt aaagactata ttcgcggcgt tgtgacaatt
     3361 taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     3421 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     3481 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     3541 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     3601 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     3661 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     3721 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     3781 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     3841 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     3901 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac
     3961 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
     4021 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     4081 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     4141 accgaacagg cttatgtcaa ctggg
//
