LOCUS       sequence-456111-       12112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CCAT1 transgene with fluorescent protein mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Caragine CM, Le VT, Mustafa M, Diaz BJ, Morris JA, Muller S, 
            Mendez-Mancilla A, Geller E, Liscovitch-Brauer N, Sanjana NE
  TITLE     Comprehensive dissection of cis-regulatory elements in a 2.8 Mb 
            topologically associated domain in six human cancers.
  JOURNAL   Nat Commun. 2025 Feb 13;16(1):1611. doi: 10.1038/s41467-025-56568-5.
  PUBMED    39948336
REFERENCE   2  (bases 1 to 12112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-13"; volume: "16(1):1611. doi"; pages: " 
            10.1038/s41467-025-56568-5"
COMMENT     Sequence Label: pLentiCCAT1-Red
FEATURES             Location/Qualifiers
     source          1..12112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1288..1297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1990..2037
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    2062..2650
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2115..2135)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2533..2544)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2722..2955
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3033..3154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3070..3089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3124..3143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3194..3329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3349..3368)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3350..3368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3378..3395)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3378..3394)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3387..3409)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3536..3991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3623..3642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3833..3854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4017..4121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4122..4190,4191..4982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4340..4359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5153..5741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5642..5661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5895..5912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6029..6050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6065..6070,6071..6088,6089..6095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6103..6119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6108..6130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6127..6143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6127..6143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6162..6182
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6164..6182
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6210..6436
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6437..6617
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6664..6789
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7282..7515
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7700..7744
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7975..8185
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7997..8193
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8044..8179
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8238..8462
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8451..8468
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        complement(11310..11513)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11339..11359)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        11514..11893
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    11953..12070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgatgcc accatggtat cagaactgat taaggaaaat
     1321 atgcatatga agctctatat ggaaggaacc gtaaacaatc accacttcaa atgtacgtca
     1381 gaaggagagg gtaaaccgta tgaagggaca cagaccatgc ggataaaagc tgtagaagga
     1441 ggaccattgc cttttgcatt cgatattttg gcaacatctt ttatgtacgg atcaaagact
     1501 ttcataaacc acactcaagg gatacctgac ttttttaagc agtcttttcc agagggcttc
     1561 acatgggaga gagtaacgac gtatgaggat ggaggggttc tgacagcaac tcaagatacc
     1621 agcctccagg atggttgtct catctacaac gtcaagataa gaggcgtgaa ctttccctca
     1681 aatggtccag ttatgcagaa gaagaccctt ggatgggagg cgagcaccga aacactctac
     1741 ccagcagacg gtggacttga agggcgagcc gacatggctc tgaagctggt tggtggtgga
     1801 catctgatat gcaatctcaa gacgacgtac aggagcaaaa agcccgccaa aaatctgaag
     1861 atgccaggcg tatattatgt tgaccggcga ctcgaaagga taaaagaggc agataaggag
     1921 acgtatgttg aacaacatga agtcgccgtg gcacgatatt gtgatctgcc cagcaaactt
     1981 gggcatagga agcgacctgc cgccacaaag aaggctggac aggctaagaa gaagaaatga
     2041 ggatccacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     2101 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2161 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2221 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2281 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2341 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2401 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2461 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2521 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2581 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2641 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     2701 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     2761 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2821 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2881 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2941 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     3001 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     3061 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3121 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     3181 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3241 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3301 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     3361 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3421 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3481 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     3541 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3601 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3661 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3721 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3781 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3841 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3901 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3961 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     4021 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4081 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4141 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4201 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4261 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4321 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4381 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4441 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4501 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4561 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4621 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4681 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4741 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4801 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4861 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4921 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4981 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5041 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5101 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5161 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5221 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5281 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5341 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5401 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5461 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5521 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5581 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5641 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5701 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5761 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5821 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5881 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5941 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6001 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6061 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6121 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     6181 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     6241 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     6301 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     6361 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     6421 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6481 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6541 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6601 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     6661 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     6721 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     6781 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     6841 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     6901 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     6961 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     7021 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     7081 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     7141 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     7201 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     7261 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7321 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     7381 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7441 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7501 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7561 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7621 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     7681 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     7741 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     7801 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     7861 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     7921 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7981 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8041 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8101 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8161 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac cccagctggt
     8221 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
     8281 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
     8341 cagacaatgc gatgcaattt tctcatttta ttaggaaagg acagtgggag tggcaccttc
     8401 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     8461 agtcgaggct gatcagcggg tttccaagac ttaatatact tatatttata ttaaaagaaa
     8521 taaatatcag atgattacaa tttggttgaa ctaagataca cagtagaata tggaactaat
     8581 atccaatatc acaaagtatt ctagcgagcc ttcctacaga aagaattgtg ggtggctggg
     8641 gagtaggcat tagctactat gtgagtgcag agaatactca gccttcttcc agatggtgag
     8701 ctaaagttca aagatcaagt cacgtacaca ccttctttct catcccaggt cctagtctgc
     8761 ttgaattcaa atggccatcc acaccttgcc tgaaatactt gcaataatta agatacggct
     8821 ttctgcctgc ttggggtttg gtccacaatt cccttaagag gcctcatttc aattaggact
     8881 cacacatccc ttcaacagta attttgtgtc aggcttggtt agcaactcaa ggctcaagca
     8941 taaatgggac agaattcttt tccttttgaa actcaccaat atagtgattg tagcaactag
     9001 ctacattgtt tttgtttttt tttccccctc aattctaagc actatgcaaa ggctttaaag
     9061 cagtggtccc aagccttttt ggcaccaggg accagttttg tggaagacaa ttttgtgaaa
     9121 agacaaaatg tggaagaccg tggactggga tggtttgggg atgattcaag cacattacat
     9181 ttgttgtgca ctgtgtttct attattatta cattgtatta tataatgaaa taattataca
     9241 actcaccata atgtagaatc agtggaagcc ctgagcttgt ttcctgcaac tagacactcc
     9301 catctagggg tgatgggaga cggtgacagg tcattaggca ttagattctc ataaggagcg
     9361 cacaacctag atccctcgca tgtgcagttc atgacagggt ttgtgctgct atgagaattt
     9421 aatgccactg ctgatctgac aggaggtgga gctcaggcag taaggtgagc aatggggagc
     9481 agctgtaaat aacgctgatc tcactcaccc actgcttacc tcctgctgtg tggcccagtt
     9541 cctaacaggc cacaaaatgg tacctgtctg tgtccccagg gttggggacc actgccttaa
     9601 aggccttcat ctcattcagt tttcatcaaa attctgtgtg gtaggtactc tcattagacc
     9661 cattttatgg gtaaggaact gaggtaaaat tggttatata acttgcctaa aataagtcaa
     9721 gtctctgatg agagggccag gattcaagtt caagcagtct gactccaaaa tctcaaagca
     9781 cttctgtggt aggaaagaga aatgaatgga atggcataga gtcatctaaa tgactgcagt
     9841 aggaagggag catttcccgt gtgcagtgtt attttttccg ggctttgaaa gataaatagg
     9901 agcatgcagt taaaaaagag aggagaaggc attctaggca gaaaggccag tgcttacaca
     9961 gaatctcaga attgtaacag ttcctattac accctggcag agtgatgcca aggctgttat
    10021 tgtcaagcac cctcctgcct cccagtgggg ttgagaaggg gtgaagggac actggcagaa
    10081 gtgaagctgg gaagtttgca cttgctagtt gggacttgca tagccatctt ctcaatgcca
    10141 aagaggacct cagtctctgt gtgcgctttg ttttttgttg ttgttgttgt tgttgatttg
    10201 tggcccaggc tagagtgcgg tggtgtgatc tctgctcact gcaacctcca tctcccaggt
    10261 tcaagggatt ctcctgcctc agactcccaa gtagcttgga ttacaggcgc ccgccaccac
    10321 acctggataa tttttatact tttagtaggg gtggagtttc accgcgttgg ccaggctggt
    10381 ctgtgtgtgc tttggatttg agacactctg atgatttaga gttgaaaatg ggagtagatt
    10441 gggtgatcct ctagttatca tgtttagtca aatcagatcc gtgcattcaa aatcataagg
    10501 caagttttcc tgtgtggctc agtaacatcc ttaaagaaat agttctgatg tccatccggt
    10561 gttttttcag aaagagcgtc agggttgaca gtagctgtga tgctccagat ggagctgcgg
    10621 ataacagcat ataagtttca gggcagtggt tgaggggctg tgggagggtg gggagggaag
    10681 atggatgact tttctcaacc atctgtattt gattggaata ttgtgtgact tgtgaaatag
    10741 aattaaagat atgatcttct tatggtcttc tcacagtttt caagggattt taggagaaaa
    10801 cgcttagcca tacaagccac ctggtaagtg gcagggctgg ccaggtcagt gcaacttcaa
    10861 agtcgatgtt gtcagtgaat gctccagatg gattgcagag aagaccaaag ttcatgtctc
    10921 ggcacctttc ccaatgtaca gggcttattg ttgggacaga gtagtgcctg gcctagaagt
    10981 taaacattca tccagctagc tattaaggct tgaatgcctt caaagaacag catggatttt
    11041 tctgtgaatc gtgagcgttt tcgcaatgct taacacggca gctggtaatg atgattgctc
    11101 ctgtttccct ttgaatttcg tgttcgttta cttaacaggg cattgctaat ctagaagaag
    11161 ggagtgggtg aagaatccca tttgtaaaga ggatagcagg ttaatgaaaa gaagcagagg
    11221 tatgcgtagg tgatagtgtt tctacaaggc tcggttcaat tggtatgatt taaagcggat
    11281 ctctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
    11341 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
    11401 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
    11461 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
    11521 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
    11581 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
    11641 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
    11701 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
    11761 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
    11821 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagtc
    11881 aataatcaat gtcaacgcgt atatctggcc cgtattctag atcttgagac aaatggcagt
    11941 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    12001 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    12061 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc gg
//
