LOCUS       sequence-456130-        9930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MAP4K5
FEATURES             Location/Qualifiers
     source          1..9930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4762..5336,5337..5339,5340..5348)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4929..4946)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5156..5175
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5787..6375
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5840..5860)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6258..6269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6447..6680
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6758..6879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6795..6814)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6849..6868
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6919..7054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7074..7093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7075..7093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7103..7120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7103..7119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7112..7134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7261..7716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7348..7367)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7558..7579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7742..7846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7847..7915,7916..8707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8065..8084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8878..9466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9367..9386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9620..9637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9754..9775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9790..9795,9796..9813,9814..9820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9828..9844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9833..9855
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9852..9868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9852..9868
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9887..9907
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9889..9907
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccttacgac
     2161 gttcctgatt acgcgggata tccctacgac gtgccagatt acgcgggtag tggtgaggcc
     2221 ccgctgcggc ctgccgcgga catcctgagg cggaacccgc agcaggacta cgaactcgtc
     2281 cagagggtcg gcagcggcac ctacggggac gtctataagg ccagaaatgt acacacagga
     2341 gagctggctg cagtaaaaat cattaaattg gagcctggag atgatttttc tttgattcaa
     2401 caagaaatat ttatggttaa agaatgtaaa cattgtaaca tcgttgccta ctttgggagt
     2461 tatcttagtc gggaaaaact atggatttgt atggaatact gtggtggcgg atcacttcaa
     2521 gatatttacc atgttactgg accattatca gaattgcaaa tagcctatgt atgcagagaa
     2581 accttacagg gtcttgccta tttgcatact aaaggcaaaa tgcatagaga tatcaaaggt
     2641 gctaatattt tattgacaga ccatggcgat gtaaaattag ctgactttgg tgtggctgca
     2701 aaaataacag ctaccattgc aaaacgaaaa tctttcattg gcacccctta ctggatggcc
     2761 ccagaagttg cagcagtaga gaagaatggt ggctacaacc aactctgtga tatctgggca
     2821 gtaggaataa cagcaattga acttggagaa cttcagccac ctatgtttga tctccaccca
     2881 atgagggctc tcttcttaat gtcaaaaagt aattttcagc ctccaaaact aaaggacaaa
     2941 acaaaatggt catcaacatt ccataatttt gtcaaaatag cactaaccaa aaacccaaaa
     3001 aaaagaccaa ctgctgaaag acttctgact cacacttttg ttgcacagcc aggtctctct
     3061 agagccctag cagttgaact gttagacaaa gtgaacaatc cagataacca cgcacattac
     3121 actgaagcag atgacgatga ctttgagccc catgcaatca ttcgtcatac cattagatct
     3181 acaaacagga atgccagagc tgaacggaca gcttcagaaa taaattttga caaattacaa
     3241 tttgaacctc ctctgagaaa agaaacagaa gcacgagatg aaatgggatt gtcatcagac
     3301 ccaaatttca tgttacagtg gaatcctttt gttgatggtg caaatactgg caaatcaacc
     3361 tcaaaacgtg caataccacc tcccctacct cctaagccaa ggataagcag ttaccctgaa
     3421 gacaactttc cggatgaaga aaaagcatca accataaaac attgtcctga ttcagaaagc
     3481 agagctcccc aaattctcag aagacagagt agcccaagtt gtgggcctgt ggcagagact
     3541 tcttctattg gaaatggtga tggtatttca aaactgatga gtgaaaatac agaaggatca
     3601 gcacaagcac cacagttacc acgaaaaaag gacaaacgag acttccctaa accagccatc
     3661 aatggccttc cacccacccc aaaagttctg atgggagcat gcttttcaaa agtttttgat
     3721 ggctgtcctt tgaaaattaa ttgtgcaaca tcctggatac atcctgatac aaaagatcag
     3781 tacattattt ttggaactga agatggtatt tacacactga atctcaatga gctacatgag
     3841 gcaacgatgg aacagttatt tccacggaag tgtacttggc tgtatgttat caataatact
     3901 ttaatgtcat tatcagaagg aaaaaccttt cagctctact ctcacaatct tatagctttg
     3961 tttgaacatg ccaaaaaacc aggattagct gcccatattc aaactcacag gtttccagac
     4021 cgaatactac caagaaaatt cgctttaaca acaaagattc ctgatacaaa aggctgccac
     4081 aaatgttgca tagtcagaaa cccttacacg ggacataaat acctctgtgg agctttacag
     4141 tctggaattg ttttacttca gtggtatgag ccaatgcaga aattcatgtt gataaagcac
     4201 tttgattttc ctttgccaag tcctttgaat gtttttgaaa tgctggtgat acctgaacag
     4261 gaatacccta tggtctgtgt agctattagc aaaggcactg aatcgaatca ggtagttcag
     4321 tttgagacaa tcaatttgaa ctctgcatct tcatggttta cagaaattgg tgcaggcagc
     4381 cagcagttag attccattca tgtaacacag ttggagagag ataccgtttt agtgtgttta
     4441 gacaaatttg tgaaaattgt aaatctacaa ggaaaattaa aatcaagtaa gaaactggcc
     4501 tctgagttaa gttttgattt tcgcattgaa tctgtagtat gccttcaaga cagtgtgttg
     4561 gctttctgga aacatgggat gcagggtaaa agcttcaagt cagatgaggt tacccaggag
     4621 atttcagatg aaacaagagt tttccgctta ttaggatcag acagggttgt cgttttggaa
     4681 agtaggccaa cagaaaatcc tactgcacac agcaatctct acatcttggc tggacatgaa
     4741 aatagttact gagcaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     4801 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     4861 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4921 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4981 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     5041 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     5101 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     5161 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     5221 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     5281 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     5341 ccacaaccat gaagaccttc aacatctctc agcaggacct tgagctggtg gaagttgcca
     5401 ctgagaaaat caccatgctc tatgaggaca acaagcacca tgttggtgct gccatcagga
     5461 ccaagacagg agaaatcatc tctgctgtcc acattgaagc ctacattggc agggtcactg
     5521 tgtgtgcaga ggccattgcc attgggtctg cagtctccaa tgggcagaag gactttgaca
     5581 ccattgtggc tgtgaggcac ccctacagtg atgaggtgga caggtccatc agagtggtgt
     5641 ccccctgtgg catgtgcagg gagctcatct cagactatgc ccctgattgc tttgttctga
     5701 ttgagatgaa tggcaagctg gtcaagacaa ccattgagga gctgattcca ctgaaataca
     5761 ccagaaacta aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5821 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5881 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5941 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6001 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6061 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6121 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6181 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6241 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6301 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6361 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     6421 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6481 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6661 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     6721 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7021 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     7081 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7141 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7201 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     7261 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7321 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7381 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7441 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7501 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7621 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7681 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9721 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9781 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9841 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9901 ctaaagggaa caaaagctgg agctgcaagc
//
