LOCUS       sequence-456131-       10558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MAP4K7
FEATURES             Location/Qualifiers
     source          1..10558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1978..2189
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    2190..2214
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2224..2250
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2254..2280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6361..6385)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6403..6991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6456..6476)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6874..6885)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7079..7312
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7384..7505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7421..7440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7475..7494
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7545..7680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7700..7719)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7701..7719)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7729..7746)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7729..7745)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7738..7760)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7887..8342
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7974..7993)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8184..8205
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8368..8472
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8473..8541,8542..9333)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8691..8710)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9504..10092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9993..10012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10246..10263
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10380..10401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10416..10421,10422..10439,10440..10446)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10454..10470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10459..10481
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10478..10494
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10478..10494
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10513..10533
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10515..10533
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg caagtttgta caaaaaagca ggctgccacc
     2221 atgtaccctt acgacgttcc tgattacgcg ggatatccct acgacgtgcc agattacgcg
     2281 gcgagcgact ccccggctcg aagcctggat gaaatagatc tctcggctct gagggacccc
     2341 gcagggatct ttgaattggt ggaacttgtt ggaaatggaa catacgggca agtttataag
     2401 ggtcgtcatg tcaaaacggg ccagcttgca gccatcaagg ttatggatgt cacaggggat
     2461 gaagaggaag aaatcaaaca agaaattaac atgttgaaga aatattctca tcaccggaat
     2521 attgctacat actatggtgc ttttatcaaa aagaacccac caggcatgga tgaccaactt
     2581 tggttggtga tggagttttg tggtgctggc tctgtcaccg acctgatcaa gaacacaaaa
     2641 ggtaacacgt tgaaagagga gtggattgca tacatctgca gggaaatctt acgggggctg
     2701 agtcacctgc accagcataa agtgattcat cgagatatta aagggcaaaa tgtcttgctg
     2761 actgaaaatg cagaagttaa actagtggac tttggagtca gtgctcagct tgatcgaaca
     2821 gtgggcagga ggaatacttt cattggaact ccctactgga tggcaccaga agttattgcc
     2881 tgtgatgaaa acccagatgc cacatatgat ttcaagagtg acttgtggtc tttgggtatc
     2941 accgccattg aaatggcaga aggtgctccc cctctctgtg acatgcaccc catgagagct
     3001 ctcttcctca tcccccggaa cccagcgcct cggctgaagt ctaagaagtg gtcaaaaaaa
     3061 ttccagtcat ttattgagag ctgcttggta aagaatcaca gccagcgacc agcaacagaa
     3121 caattgatga agcatccatt tatacgagac caacctaatg agcgacaggt ccgcattcaa
     3181 ctcaaggacc atattgatag aacaaagaag aagcgaggag aaaaagatga gacagagtat
     3241 gagtacagtg gaagtgagga agaagaggag gagaatgact caggagagcc cagctccatc
     3301 ctgaatctgc caggggagtc gacgctgcgg agggactttc tgaggctgca gctggccaac
     3361 aaggagcgtt ctgaggccct acggaggcag cagctggagc agcagcagcg ggagaatgag
     3421 gagcacaagc ggcagctgct ggccgagcgt cagaagcgca tcgaggagca gaaagagcag
     3481 aggcggcggc tggaggagca acaaaggcga gagaaggagc tgcggaagca gcaggagagg
     3541 gagcagcgcc ggcactatga ggagcagatg cgccgggagg aggagaggag gcgtgcggag
     3601 catgaacagg aatacatcag gcgacagtta gaggaggagc agagacagtt agagatcttg
     3661 cagcagcagc tactgcatga acaagctcta cttctggaat ataagcgcaa acaattggaa
     3721 gaacagagac aagcagaaag actgcagagg cagctaaagc aagaaagaga ctacttagtt
     3781 tcccttcagc atcagcggca ggagcagagg cctgtggaga agaagccact gtaccattac
     3841 aaagaaggaa tgagtcctag tgagaagcca gcatgggcca aggaggtaga agaacggtca
     3901 aggctcaacc ggcaaagttc ccctgccatg cctcacaagg ttgccaacag gatatctgac
     3961 cccaacctgc ccccaaggtc ggagtccttc agcattagtg gagttcagcc tgctcgaaca
     4021 ccccccatgc tcagaccagt cgatccccag atcccacatc tggtagctgt aaaatcccag
     4081 ggacctgcct tgaccgcctc ccagtcagtg cacgagcagc ccacaaaggg cctctctggg
     4141 tttcaggagg ctctgaacgt gacctcccac cgcgtggaga tgccacgcca gaactcagat
     4201 cccacctcgg aaaatcctcc tctccccact cgcattgaaa agtttgaccg aagctcttgg
     4261 ttacgacagg aagaagacat tccaccaaag gtgcctcaaa gaacaacttc tatatcccca
     4321 gcattagcca gaaagaattc tcctgggaat ggtagtgctc tgggacccag actaggatct
     4381 caacccatca gagcaagcaa ccctgatctc cggagaactg agcccatctt ggagagcccc
     4441 ttgcagagga ccagcagtgg cagttcctcc agctccagca cccctagctc ccagcccagc
     4501 tcccaaggag gctcccagcc tggatcacaa gcaggatcca gtgaacgcac cagagttcga
     4561 gccaacagta agtcagaagg atcacctgtg ctcccccatg agcctgcgaa ggtgaaacca
     4621 gaagaatcca gggacattac ccggcccagt cgaccagcta gctacaaaaa agctatagat
     4681 gaggatctga cggcattagc caaagaacta agagaactcc ggattgaaga aacaaaccgc
     4741 ccaatgaaga aggtgactga ttactcctcc tccagtgagg agtcagaaag tagcgaggaa
     4801 gaggaggaag atggagagag cgagacccat gatgggacag tggctgtcag cgacataccc
     4861 agactgatac caacaggagc tccaggcagc aacgagcagt acaatgtggg aatggtgggg
     4921 acgcatgggc tggagacctc tcatgcggac agtttcagcg gcagtatttc aagagaagga
     4981 accttgatga ttagagagac gtctggagag aagaagcgat ctggccacag tgacagcaat
     5041 ggctttgctg gccacatcaa cctccctgac ctggtgcagc agagccattc tccagctgga
     5101 accccgactg agggactggg gcgcgtctca acccattccc aggagatgga ctctgggact
     5161 gaatatggca tggggagcag caccaaagcc tccttcaccc cctttgtgga ccccagagta
     5221 taccagacgt ctcccactga tgaagatgaa gaggatgagg aatcatcagc cgcagctctg
     5281 tttactagcg aacttcttag gcaagaacag gccaaactca atgaagcaag aaagatttcg
     5341 gtggtaaatg taaacccaac caacattcgg cctcatagcg acacaccaga aatcagaaaa
     5401 tacaagaaac gattcaactc agaaatactt tgtgcagctc tgtggggtgt aaaccttctg
     5461 gtggggactg aaaatggcct gatgcttttg gaccgaagtg ggcaaggcaa agtctataat
     5521 ctgatcaacc ggaggcgatt tcagcagatg gatgtgctag agggactgaa tgtccttgtg
     5581 acaatttcag gaaagaagaa taagctacga gtttactatc tttcatggtt aagaaacaga
     5641 atactacata atgacccaga agtagaaaag aaacaaggct ggatcactgt tggggacttg
     5701 gaaggctgta tacattataa agttgttaaa tatgaaagga tcaaattttt ggtgattgcc
     5761 ttaaagaatg ctgtggaaat atatgcttgg gctcctaaac cgtatcataa attcatggca
     5821 tttaagtctt ttgcagatct ccagcacaag cctctgctag ttgatctcac ggtagaagaa
     5881 ggtcaaagat taaaggttat ttttggttca cacactggtt tccatgtaat tgatgttgat
     5941 tcaggaaact cttatgatat ctacatacca tctcatattc agggcaatat cactcctcat
     6001 gctattgtca tcttgcctaa aacagatgga atggaaatgc ttgtttgcta tgaggatgag
     6061 ggggtgtatg taaacaccta tggccggata actaaggatg tggtgctcca atggggagaa
     6121 atgcccacgt ctgtggccta cattcattcc aatcagataa tgggctgggg cgagaaagct
     6181 attgagatcc ggtcagtgga aacaggacat ttggatggag tatttatgca taagcgagct
     6241 caaaggttaa agtttctatg tgaaagaaat gataaggtat tttttgcatc cgtgcgatct
     6301 ggaggaagta gccaagtgtt tttcatgacc ctcaacagaa attccatgat gaactggtga
     6361 acccagcttt cttgtacaaa gtggtgataa tcgaattccg ataatcaacc tctggattac
     6421 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6481 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6541 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6601 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6661 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6721 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6781 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6841 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6901 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6961 agtcggatct ccctttgggc cgcctccccg catcgggaat tcccgcggtt cgctttaaga
     7021 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7081 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7141 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7201 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7261 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7321 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7381 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7441 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7501 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     7561 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7621 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7681 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     7741 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7801 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7861 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7921 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7981 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8041 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8101 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8161 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8221 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8281 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8341 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8401 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8461 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8521 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8581 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8641 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8701 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8761 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8821 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8881 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8941 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9001 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9061 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9121 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9181 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9241 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9301 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9361 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9421 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9481 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9541 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9601 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9661 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9721 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9781 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9841 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9901 gaaagcgcca cgcttcccga agagagaaag gcggacaggt atccggtaag cggcagggtc
     9961 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10021 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10081 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10141 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10201 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10261 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10321 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    10381 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    10441 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    10501 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagctt
//
