LOCUS       sequence-456133-        8096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse EHMT1 with deletion of aa 1185-1298.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S.
  TITLE     A cytoplasmic form of EHMT1N methylates viral proteins to enable 
            inclusion body maturation and efficient viral replication
  JOURNAL   PLOS Biology 22(11): e3002871.
REFERENCE   2  (bases 1 to 8096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS 
            Biology 22(11): e3002871."
COMMENT     Sequence Label: EGFP_EHMT1B1-3
FEATURES             Location/Qualifiers
     source          1..8096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(15..218)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(44..64)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        219..522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(697..1285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(777..796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    1614..1661
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1686..1705
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1893..2687)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1893..2687)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2004..2023)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2614..2633
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2722..3079)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2736..2871
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        2888..3079
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     3036..3056
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(3081..3185)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3212..3667
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3299..3318)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3509..3530
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3674..3795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3685..3704)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3739..3758
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      7325..7334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(7350..8060,8061..8063,8064..8066))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7350..8060,8061..8063,8064..8066))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7392..7413)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7741..7760
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8000..8021
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8063..8072
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
       61 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      121 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      181 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
      241 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
      301 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
      361 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
      421 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
      481 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
      541 ctatgaacta atgaccccgt aattgattac tattaataac taatgcatgg cggtaatacg
      601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1441 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     1501 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     1561 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     1621 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     1681 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     1741 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     1801 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     1861 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     1921 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     1981 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     2041 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     2101 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     2161 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     2221 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     2281 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     2341 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     2401 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     2461 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     2521 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     2581 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     2641 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     2701 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     2761 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     2821 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     2881 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     2941 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     3001 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     3061 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     3121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3181 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     3241 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3301 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3361 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3421 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3481 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3541 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3601 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     3661 acaatttacg cgttaagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     3721 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     3781 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     3841 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     3901 cagttatcta gatccggtgg atcccgggcc cgcggtacct cgcagtatac ctctccatcc
     3961 ttattgtcaa gatcaaagag gtaagagtcc tcttcccgaa catcagcttc agagtccgaa
     4021 atcagctccc ctacgtattc gcagacaaag gtgcccagtg ggatatcctg gagggaccgc
     4081 acaccccagc ccatgtcctg tgtccgataa agctgcagcc ttgccctgag accattttgc
     4141 accacacgat tgcggcagtt cctccagcat gagcaggcat gattgcactc gaagatcaag
     4201 ggtggttctg ccatgttaaa ctcaggcaga agtcggccat ccttatcata ccagcagcgc
     4261 atgctcagct ggccacacat gcaggtgcta gaggagcagt catctacaca cacgcagtac
     4321 tgcaaatgag tgatgttcct gtcaatgttc atgggggatg tcacacagtt ctgggagaca
     4381 tacttatagt tggtaggaca cagctcactg tccacagcat tgacacaggg aatgggaatg
     4441 cgctcatacc ctcgagcgat atccctgctc accgtcttct caacagcaac gggcttgtca
     4501 ggggctgagt cccgaagtgc tttgctcatc tgcaatgcac tccacacctg cgaactgaga
     4561 cttgcacact gcaagggagt ctctccttcc ttgtttttca gagtaacatc tgaatcccga
     4621 gaaagaaaga ggacaacaca gtcgtagcga ttctccctgg ctgcgatgtg caggggtgag
     4681 tctccatgga tattcacagc atgcaggtca cacttggcag ccagaagtat ttcagctatg
     4741 tccacacagc ctgaaaatgc tgcccagtgc agacaaatgt tctcctcgtt gtcccggatg
     4801 ttgatgtcag agcccttaga cagcagcagc ttcaccagct ccacgtgctt gtactcagtg
     4861 gcccagatca taggtgtcca tccaccgtca tcctggcagt tgacatccat ctgtccattt
     4921 gaaagcagat actgaaccac atcatagtgg cctttcttgg cagccaaatg caaacatgtg
     4981 gagccctctg cgtccttcgg atccacctgt gctccagcct tgatgaggta cttcactgca
     5041 tccaagtggt tgttctctgc agcctccatc agtggggtcc gctggtcctc tgagcaagtg
     5101 tcaatattgg cacccgcctg aaccagcatg tggcagatgt ccacgtggcc agcctccgca
     5161 gcagcatgta atggggaacg cttactttgg tgctccattt tgaagttggg atcaattcca
     5221 tcaaccagca tgagaagcac cttctgcagc tcaccctgcc tggcagagaa gtacagctgc
     5281 tttgggtgga agcgaagttt cttgggtttt tcagagtcta gagcgattag agcactttcc
     5341 aaggtttcct ttcctgggcc ctgggaaagg ccagatgtcg gcctcacgag cccagccggt
     5401 ccggcagggt cgaagcactc tggtgcctgg ggggctgtgc tctgcgatct ttcatcctct
     5461 ggggctccag caatgctgcc cgtggtcgtg tcagccctgc cttcagcagc caggctcttc
     5521 tcctgtccag gggctagggt cactgtggaa gttgtgtctg cttttgctat ggtcacctct
     5581 ttggccttgg aagcttcttc cccacaatgg ggacagtagc tggcattgtt gactcgagag
     5641 gcacagtcct tatggaaacg atgagagatg ctgctctcag gctggcattc catgaagtta
     5701 ccttcgtgat ccacgctctc agtggccatg cactggttgt tggccagggt gctgatctcg
     5761 cggctcttgg gggtttccat tcggcagctg cagagaggca cttcctggag gccatccgtc
     5821 cccagcacat ccggaccact ggccagccct tcattttctg tctgggaaga cagaattcct
     5881 ctgacacgga gatccaggga atccagggaa acttccatat agccagcgct gtctcccaga
     5941 gctgcctgct ccatgcttcc tggagatgac ttacaagcct cggaaccaag catgctgctc
     6001 ggcttctttc tacttctccg cctccttttc cgagcaggtt tgatccaggg gctgtcagtc
     6061 ttccctctcc tcttgagaaa tttcttcttg atactggatt cagaactcag gtcagactcg
     6121 tctccaccat cttcatcctc ccctgtgtcg ggagactcct gttcctcctc tgaatctcca
     6181 tccatctttg cagcccggtc agtctcagac atgctctctt tagaagtcct actatcctcg
     6241 gtgggaaatg cagccgcttg ctcagctcca tggtcctcgt catcctccag ctcatcggag
     6301 tcttcatctt ctgagtccat ctccaagctc tcgccattta catgcagggc actatcatct
     6361 aaggctttct cgcccttggc gcccacagtg gaatgggtta tgcttttaaa catctcaatc
     6421 accgtcctct gttttaatac tttggtcttt ttcttgggaa ctaaactata ggttcccatt
     6481 cttcgttttt tcttccgaga tactgcagct gctgtctggg actttgtggt ggccatgtag
     6541 cactgattct gaggtagcga ctggtggagg gctgggaagg ttggtaaaag ctgctgtcgt
     6601 ccacattcag aaatgtttct gttgatatcc tcttttggtt ccttatggtc ttgaacttct
     6661 ctatggtctt tgctggctgc atgcaggccc aagatggact tcggcatggt cttgcgtgcc
     6721 ctgtgaaccc tgacatcagt gcccgaggct gtaggctttc tgtcctctgt gtctgcactc
     6781 ccttccccag gcacagtggg tgctgtggtt ggtgtctgag gaagtgcact cagagtcctg
     6841 catttactgg ctcctccagg aagagttttt gcagcatgac caggaagcga ggaggttaga
     6901 atgttgggag tcctcaacgg ctgcccctgc agggctggtt tatttaagaa atatccattg
     6961 ctgccaatga cagatgtctg catgaagtcg tcagctgtga catggttttg cttccccact
     7021 tctgtgtctc tttctgaaac cccattttct gccacccgag acactctgtt ggtgccttcc
     7081 tgtgggctag ctcttgtgtt ctcctgagtg tgtttgggtg catttagatg gctgggatcc
     7141 ccactctttt cacaagaccc atttgtttct ccatctgcag ccatgggagt ctctccttct
     7201 tgtttctctg tggaaccttc atcagcagcc ataggtgtat cttcccttag cagctcagtt
     7261 ttcatgcagc aatcctgctt ggtctcttgc ttggccagaa ctgcctgctc agcatcagcg
     7321 gcggccatgg tggcagatct gagtccggac ttgtacagct cgtccatgcc gagagtgatc
     7381 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     7441 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     7501 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
     7561 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
     7621 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     7681 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     7741 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
     7801 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
     7861 acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     7921 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
     7981 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     8041 aacagctcct cgcccttgct caccatggtg gcgaccggta gcgctagcgg atctga
//
