LOCUS       sequence-456135-        9513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-YAP-5A
FEATURES             Location/Qualifiers
     source          1..9513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3736..4310,4311..4313,4314..4322)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3903..3920)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4130..4149
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4323..5342
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    5370..5958
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5423..5443)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5841..5852)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6030..6263
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6341..6462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6378..6397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6432..6451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6502..6637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6657..6676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6658..6676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6686..6703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6686..6702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6695..6717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6844..7299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6931..6950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7141..7162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7325..7429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7430..7498,7499..8290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7648..7667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8461..9049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8950..8969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9203..9220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9337..9358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9373..9378,9379..9396,9397..9403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9411..9427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9416..9438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9435..9451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9435..9451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9470..9490
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9472..9490
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccttacgac
     2161 gttcctgatt acgcgggata tccctacgac gtgccagatt acgcgggtag tggtgaccct
     2221 gggcaacagc cccctccgca gccggcacca cagggccagg gacaacctcc gtctcagccg
     2281 cctcaagggc aagggccacc gtcaggacct ggacaacctg cgccagcagc cactcaagcg
     2341 gccccgcagg ctccgcccgc aggacaccaa atagtccacg tgagaggcga ctccgagacg
     2401 gacctcgagg ccctctttaa tgctgttatg aaccctaaga cagcgaacgt gcctcaaact
     2461 gtgcctatgc gattgcgaaa gctgccagat tcctttttca agccgccaga acccaaatcc
     2521 cactcccgcc aggcttccac cgacgctggt acggccgggg cacttactcc ccaacatgtt
     2581 cgggctcata gcgctcccgc ttccctccag cttggtgctg tttctccggg gacgctcacc
     2641 ccgaccggtg tagtttccgg acctgctgct actccgactg ctcaacattt gcgccaagct
     2701 agctttgaga ttccggacga tgtgcctctg ccggctggct gggaaatggc aaaaacatcc
     2761 agtggtcaaa gatattttct caatcatatt gaccagacga ccacctggca agatccgcgg
     2821 aaagctatgc tctcacagat gaacgttacc gctccgacct ctcctcccgt acaacaaaac
     2881 atgatgaact cagcgtcagg gcccctccca gatggctggg aacaggcaat gactcaggac
     2941 ggcgagattt attacattaa tcacaaaaac aagaccacta gttggctgga ccctcggctt
     3001 gatccacgat tcgctatgaa tcagagaata tcccagagcg ctccggttaa gcagccacct
     3061 cccctggcac cccagagtcc tcaaggcgga gtcatgggcg ggagtaatag caaccagcag
     3121 cagcagatgc gacttcagca actccaaatg gaaaaagaaa gacttaggct gaaacaacag
     3181 gaacttctgc gccaggcaat gcggaacatt aatcccagca cagccaatag tccaaagtgc
     3241 caagaactgg ccttgcgcgc tcaacttcca gctttggagc aagacggagg aactcaaaac
     3301 ccggttagct ctcctggtat gtcccaagag ttgcggacaa tgactacgaa tagtagcgat
     3361 cccttcttga atagtggcac ttaccactca agggacgagt ccgctgacag tgggttgtca
     3421 atgtccagtt atagcgtgcc ccgcacccct gatgacttcc tgaactcagt agacgaaatg
     3481 gacacaggcg acacgatcaa ccaatcaaca cttccttccc agcagaatag attcccagat
     3541 tatctggaag ccatcccggg tacgaacgtc gacttgggta ccttggaagg agatgggatg
     3601 aatatcgaag gtgaggagct gatgccatct ttgcaagaag cattgagctc tgatatcctt
     3661 aacgacatgg agagtgtcct cgccgcgact aagttggaca aagagtcttt cctgacttgg
     3721 ctttgagcaa ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     3781 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3841 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3901 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3961 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4021 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4081 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4141 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4201 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4261 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     4321 ccatgaaaaa gcctgaactc accgctacct ctgtcgagaa gtttctgatc gaaaagttcg
     4381 acagtgttag cgacctgatg cagctctccg agggcgaaga atctcgggct ttcagcttcg
     4441 atgtgggagg gcgtggatat gtcctgcggg tgaatagctg cgccgatggt ttctacaaag
     4501 atcgctatgt ttatcggcac tttgcatccg ccgctctccc tattcccgaa gtgcttgaca
     4561 ttggggagtt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacct
     4621 tgcaagacct gcctgaaacc gaactgcccg ctgttctcca gcccgtcgcc gaggccatgg
     4681 atgccatcgc tgccgccgat cttagccaga ccagcgggtt cggcccattc ggacctcaag
     4741 gaatcggtca atacactaca tggcgcgatt tcatctgcgc tattgctgat ccccatgtgt
     4801 atcactggca aactgtgatg gacgacaccg tcagtgcctc cgtcgcccag gctctcgatg
     4861 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gccgatttcg
     4921 gctccaacaa tgtcctgacc gacaatggcc gcataacagc cgtcattgac tggagcgagg
     4981 ccatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccctggttgg
     5041 cttgtatgga gcagcagacc cgctacttcg agcggaggca tcccgagctt gcaggatctc
     5101 ctcggctccg ggcttatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     5161 acggcaattt cgatgatgca gcttgggctc agggtcgctg cgacgcaatc gtccggtccg
     5221 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgctgccgtc tggaccgatg
     5281 gctgtgtgga agtgctcgcc gatagtggaa acagacgccc cagcactcgt cctagggcaa
     5341 aggatctgca gtagacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     5401 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5461 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5521 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5581 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcaactcc
     5641 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5701 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5761 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5821 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5881 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5941 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     6001 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6061 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6121 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6181 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6241 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     6301 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6361 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6421 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     6481 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6541 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6601 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     6661 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6721 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6781 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6841 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6901 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6961 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7021 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7081 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7141 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7201 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7261 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7321 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7381 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7441 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7501 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7561 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7621 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7681 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7741 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7801 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7861 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7921 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7981 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8041 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8101 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8161 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8221 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8281 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8341 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8581 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9241 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9301 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9361 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9421 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     9481 tcactaaagg gaacaaaagc tggagctgca agc
//
