LOCUS       sequence-456136-        8904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-YAP-wt
FEATURES             Location/Qualifiers
     source          1..8904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3736..4310,4311..4313,4314..4322)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3903..3920)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4130..4149
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    4761..5349
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4814..4834)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5232..5243)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5421..5654
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5732..5853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5769..5788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5823..5842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5893..6028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6048..6067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6049..6067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6077..6094)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6077..6093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6086..6108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6235..6690
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6322..6341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6532..6553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6716..6820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6821..6889,6890..7681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7039..7058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7852..8440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8341..8360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8594..8611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8728..8749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8764..8769,8770..8787,8788..8794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8802..8818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8807..8829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8826..8842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8826..8842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8861..8881
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8863..8881
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccttacgac
     2161 gttcctgatt acgcgggata tccctacgac gtgccagatt acgcgggtag tggtgaccct
     2221 gggcaacagc cccctccgca gccggcacca cagggccagg gacaacctcc gtctcagccg
     2281 cctcaagggc aagggccacc gtcaggacct ggacaacctg cgccagcagc cactcaagcg
     2341 gccccgcagg ctccgcccgc aggacaccaa atagtccacg tgagaggcga ctccgagacg
     2401 gacctcgagg ccctctttaa tgctgttatg aaccctaaga cagcgaacgt gcctcaaact
     2461 gtgcctatgc gattgcgaaa gctgccagat tcctttttca agccgccaga acccaaatcc
     2521 cactcccgcc aggcttccac cgacgctggt acggccgggg cacttactcc ccaacatgtt
     2581 cgggctcata gctcacccgc ttccctccag cttggtgctg tttctccggg gacgctcacc
     2641 ccgaccggtg tagtttccgg acctgctgct actccgactg ctcaacattt gcgccaatcc
     2701 agctttgaaa ttccggacga tgtgcctctg ccggctggct gggaaatggc aaaaacatcc
     2761 agtggtcaaa gatattttct caatcatatt gaccagacga ccacctggca agatccgcgg
     2821 aaagctatgc tctcacagat gaacgttacc gctccgacct ctcctcccgt acaacaaaac
     2881 atgatgaact cagcgtcagg gcccctccca gatggctggg aacaggcaat gactcaggac
     2941 ggcgagattt attacattaa tcacaaaaac aagaccacta gttggctgga ccctcggctt
     3001 gatccacgat tcgctatgaa tcagagaata tcccagagcg ctccggttaa gcagccacct
     3061 cccctggcac cccagagtcc tcaaggcgga gtcatgggcg ggagtaatag caaccagcag
     3121 cagcagatgc gacttcagca actccaaatg gaaaaagaaa gacttaggct gaaacaacag
     3181 gaacttctgc gccaggcaat gcggaacatt aatcccagca cagccaatag tccaaagtgc
     3241 caagaactgg ccttgcgctc acaacttcca acattggagc aagacggagg aactcaaaac
     3301 ccggttagct ctcctggtat gtcccaagag ttgcggacaa tgactacgaa tagtagcgat
     3361 cccttcttga atagtggcac ttaccactca agggacgagt ccaccgacag tgggttgtca
     3421 atgtccagtt atagcgtgcc ccgcacccct gatgacttcc tgaactcagt agacgaaatg
     3481 gacacaggcg acacgatcaa ccaatcaaca cttccttccc agcagaatag attcccagat
     3541 tatctggaag ccatcccggg tacgaacgtc gacttgggta ccttggaagg agatgggatg
     3601 aatatcgaag gtgaggagct gatgccatct ttgcaagaag cattgagctc tgatatcctt
     3661 aacgacatgg agagtgtcct cgccgcgact aagttggaca aagagtcttt cctgacttgg
     3721 ctttgagcaa ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     3781 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3841 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3901 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3961 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4021 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4081 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4141 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4201 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4261 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     4321 ccatgaagac cttcaacatc tctcagcagg accttgagct ggtggaagtt gccactgaga
     4381 aaatcaccat gctctatgag gacaacaagc accatgttgg tgctgccatc aggaccaaga
     4441 caggagaaat catctctgct gtccacattg aagcctacat tggcagggtc actgtgtgtg
     4501 cagaggccat tgccattggg tctgcagtct ccaatgggca gaaggacttt gacaccattg
     4561 tggctgtgag gcacccctac agtgatgagg tggacaggtc catcagagtg gtgtccccct
     4621 gtggcatgtg cagggagctc atctcagact atgcccctga ttgctttgtt ctgattgaga
     4681 tgaatggcaa gctggtcaag acaaccattg aggagctgat tccactgaaa tacaccagaa
     4741 actaaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4801 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4861 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4921 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4981 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5041 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5101 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5161 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5221 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5281 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5341 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5401 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5461 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5641 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     5701 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     5761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5821 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     5881 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5941 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6001 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     6061 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6121 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6181 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     6241 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6301 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6361 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6421 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6481 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6541 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6601 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6661 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     6721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6781 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6961 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7021 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7201 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7381 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7441 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7681 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7741 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7801 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7861 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7921 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7981 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8041 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8101 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8161 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8221 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8281 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8341 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8401 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8461 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8521 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8581 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8641 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8701 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8761 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8821 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     8881 ggaacaaaag ctggagctgc aagc
//
