LOCUS       sequence-45614-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt6-V5
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..51)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg
       61 cgggccctct agatcaacca ctttgtacaa gaaagctggg tcggcgcgcc cacccttcag
      121 aatgcacagg cagaggctga gctccttgcg cacacggcag cggtggcact gtactacgca
      181 gcaccagtgg aagcggcaca ggcagttctc ttcgagctgc acgctctcct ggcggtgccc
      241 gcggccgcag cacagcaggt cgcagccgct gaggtccggg gcgctgctat tgcaggcgcg
      301 accgcgcgtg ccgggggagc cggtgcgtcg gttgggggcg cagaagtcgg gcgaatcggc
      361 ggcgtagagg aggtccgctc ggcccggcgg cttgagcgtg cggacggcgg gcagcagggc
      421 cttgccgtcg ttggtgccca tgacgcgtga ggcgccgtgg aagcgctcca gcagccgcgc
      481 gcccacctcg cgaaatggag gcagcttctg ccagcaggtg cgcagcgcgc atgatcccga
      541 cagcccgtgg catttgcact cggtgcgcgt gtggctccgc acggccagcc tgcccgcctc
      601 gttgttgtgc agttgcacca acgcgcggat gtctccgcgt ccccgcttgt gccgtgcgtc
      661 cataaagagc ctcgacttct cgtccccgaa gtccacgtcg tcgccgcagc ctccccactc
      721 ccaggcggcg ctgccttccg gggagcccgc ggggccaggg ggtccggggg tgccgggcag
      781 gccggagggc cggggagggg cccgcccgcg gggcgcctgg cagccgc
//
