LOCUS       sequence-456141-        7100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human EHMT1 with deletion of region between N-terminal
            300 aa and C-terminal Ank+SET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S.
  TITLE     A cytoplasmic form of EHMT1N methylates viral proteins to enable 
            inclusion body maturation and efficient viral replication
  JOURNAL   PLOS Biology 22(11): e3002871.
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS 
            Biology 22(11): e3002871."
COMMENT     Sequence Label: EGFP_delta_EHMT1
FEATURES             Location/Qualifiers
     source          1..7100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        85..276
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     233..253
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(278..382)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      409..864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(496..515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     706..727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    871..992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(882..901)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     936..955
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(3551..4261,4262..4264,4265..4267))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3551..4261,4262..4264,4265..4267))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3593..3614)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3942..3961
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4201..4222
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4264..4273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4312..4515)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4341..4361)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4516..4819
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4994..5582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5074..5093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5911..5958
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5983..6002
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6190..6984)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6190..6984)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6301..6320)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6911..6930
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(7019..7100,1..276))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(7033..7100,1..68)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
       61 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
      121 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
      181 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
      241 tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt caatattatt
      301 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
      361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
      421 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      481 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      541 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      601 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
      661 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
      721 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
      781 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
      841 acaaaatatt aacgcttaca atttacgcgt taagatacat tgatgagttt ggacaaacca
      901 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      961 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1021 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     1081 gtatggctga ttatgatcag ttatctagat ccggtggatc ccgggcccgc ggtaccgtcg
     1141 acgtgccggc acttggggga gccgcagcgg cagctgaaga gcttgccttt gatgtcccag
     1201 aagcgctctc catagtcaaa cccgagctgc tcgccggcct cgatcaggcg ggtgctgaag
     1261 aaggcgatcc gggggaaccg caggtcctgg tgggccatga acacgcgcac gggcaccagg
     1321 ttgggctcgc agtggtggtt gatgaaccgg ctgacgttcc cgtagaaccg cgcgtcgatg
     1381 cagtaaacct ccccgtctcg cagacaaagg tgcctggtgg gatgtcctgc agggaccgca
     1441 cgccccagcc catgtcccgc gtccggtaga gctgcagcct tgccctgaga ccattctgta
     1501 cgacgcgatt tcggcagttc ctccagcagg agcacgcgtg gttgcattcg aagatcaagg
     1561 gaggctccgc catgttgaac tctggcagga gccggccatc cttgtcgtac cagcagcgca
     1621 tgctgagctg gccgcacatg cagttgctgg aggagcagtc gtcgatgcac acgcagtact
     1681 gcagatgagt gatatttctg tcgatgttca tgggggacgt cacgcagttc tgagagacgt
     1741 acttgtagtt gctggggcat ggctcgctgt ccacggcgtt gacacagggg atggggatgc
     1801 gctcgtagcc tcgagcgatg tccctgctca ctatcctctc cacggggctg ggcctgtcgg
     1861 gggccgagtc ctgcagagcc ttgctcatct gcagagcgct ccacacctga gagttgaggc
     1921 tcgcacactg caggggcgtc tctccttcct tgttctttaa ggtgacatct gaatcccgag
     1981 aaagaaagag gacgacacag tcgtagcggt tctcccgggc ggcaatgtgc agtggcgagt
     2041 ctccgtggat gttcacggcg tggaggtcgc acttggcagc cagcaggatc tcggctatgt
     2101 ccacgcagcc ggagaacgcc gcccagtgca ggcaaatgtt ctcctcgttg tctcggatgt
     2161 tgatgtcaga gcccttggac agcagcagct tcacgaggtc cacgtgcttg tactctgtgg
     2221 cccagatcat gggtgtccag cctccgtcat cctgacagtt gacgtccatc tgtccatttg
     2281 aaagcaggta ctggaccact tcgtagtggc ctttcttggc agccaggtgc aaacacgtag
     2341 agccctctgc gtccttggga tccaccaggg ccccagcctt gatgaggtac ttcactgctt
     2401 ccagatggtt gttttcggct gcttccatca acggggtcct ctggtcttct gagcaggtgt
     2461 caatattagc gcccgcctga accagcatgt ggcagatgtc cacgtgtcca gcctctgccg
     2521 cggcgtgcag tggagagcgc ttattctggt gctccatttt gaagttgggg tcaattccgt
     2581 ccaccagcat gaggagcacc ttctgaagct ccccttgcct ggcggagaag tacagctgct
     2641 ttgggtggaa gtcgacagga accaggctat aggttcccat tcttcgtttt ttcttccgag
     2701 gtactgcagc tgctgtctgt gattttgtgg tggccatgta gcactggttc tgaggtagcg
     2761 actgatgaag ggatgggaag gggggtaaaa gctgctgtcg tccaaagtca gaaatgtttt
     2821 tgttgacctc ctcttttggc tccttatgat ctctagcttc tcgaacttct ctgggatctt
     2881 tactggctgc atgcaggccc acgacggact tcggcatggt cttgcgtgcc ctgtggacct
     2941 tgacgtcggc gccaggggcc gggagcttcc tgtcctctgt gtcagcactc ccctccccaa
     3001 gggtggctgg tggggcggct ggcgtctggg gaaaagcgct tggagtcctg cctttgccag
     3061 cccctccagg aagggttttt gcagcatggc caggcagcga agaggccaga gtgctggtag
     3121 tcctcaaggg ctgtgcctgt agggccggct tatttaagat gtatccgttg ctgccgatga
     3181 cagaagtctg cacaaagtcg tcggcagtga cgtggttttg cttcgccgct tctgagtctc
     3241 tttctgaaac cccattttcc gctatccgag ttagtgtgtt ggtgccatcc tgggggttga
     3301 cccttgcgct gtcctgagtg tgctttgcag catttgcatg actgctggca tcgctgtttt
     3361 cacaagaccc ataggtctca ccgtccgcag tcatgtgggc ctctcctgcc tgtttctctg
     3421 ctgagccttc gtcggcagcc ataggtgtct cttctcccag cagctcggtt ttcacacagc
     3481 aatcctgctg aggctccccc ctcgccggaa ctgcctcggc atcggcggcg gccatggatc
     3541 tgagtccgga cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     3601 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     3661 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     3721 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     3781 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     3841 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     3901 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     3961 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     4021 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     4081 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     4141 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     4201 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     4261 tcaccatggt ggcgaccggt agcgctagcg gatctgacgg ttcactaaac cagctctgct
     4321 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     4381 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     4441 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     4501 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     4561 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     4621 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     4681 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     4741 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     4801 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     4861 tgattactat taataactaa tgcatggcgg taatacggtt atccacagaa tcaggggata
     4921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5401 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5581 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5641 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5701 aatgaagttt taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg
     5761 ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg
     5821 aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt
     5881 gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat
     5941 tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt
     6001 cagttagcct ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga
     6061 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     6121 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     6181 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     6241 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     6301 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     6361 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     6421 atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc
     6481 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     6541 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     6601 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     6661 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     6721 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     6781 cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac
     6841 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     6901 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     6961 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt
     7021 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     7081 aggcggcctc ggcctctgca
//
