LOCUS       sequence-456144-       12195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 12195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MAP4K4-10D
FEATURES             Location/Qualifiers
     source          1..12195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1978..2189
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    2190..2214
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2224..2250
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2254..2280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6442..6466)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6484..7072
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6537..6557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6955..6966)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7103..7602
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7138..7161)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7535..7554
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7619..8644
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7619..8644
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             8716..8949
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9021..9142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9058..9077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9112..9131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9182..9317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9337..9356)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9338..9356)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9366..9383)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9366..9382)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9375..9397)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9524..9979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9611..9630)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9821..9842
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10005..10109
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10110..10178,10179..10970)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10328..10347)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11141..11729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11630..11649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11883..11900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12017..12038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12053..12058,12059..12076,12077..12083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12091..12107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12096..12118
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12115..12131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12115..12131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12150..12170
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12152..12170
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg caagtttgta caaaaaagca ggctgccacc
     2221 atgtaccctt acgacgttcc tgattacgcg ggatatccct acgacgtgcc agattacgcg
     2281 ggtagtggtg cgaacgactc ccctgcaaaa agtctggtgg acatcgacct ctcctccctg
     2341 cgggatcctg ctgggatttt tgagctggtg gaagtggttg gaaatggcac ctatggacaa
     2401 gtctataagg gtcgacatgt taaaacgggt cagttggcag ccatcaaagt tatggatgtc
     2461 actgaggatg aagaggaaga aatcaaactg gagataaata tgctaaagaa atactctcat
     2521 cacagaaaca ttgcaacata ttatggtgct ttcatcaaaa agagccctcc aggacatgat
     2581 gaccaactct ggcttgttat ggagttctgt ggggctgggt ccattacaga ccttgtgaag
     2641 aacaccaaag ggaacacact caaagaagac tggatcgctt acatctccag agaaatcctg
     2701 aggggactgg cacatcttca cattcatcat gtgattcacc gggatatcaa gggccagaat
     2761 gtgttgctga ctgagaatgc agaggtgaaa cttgttgact ttggtgtgag tgctcagctg
     2821 gacaggactg tggggcggag aaatacgttc ataggcactc cctactggat ggctcctgag
     2881 gtcatcgcct gtgatgagaa cccagatgcc acctatgatt acagaagtga tctttggtct
     2941 tgtggcatta cagccattga gatggcagaa ggtgctcccc ctctctgtga catgcatcca
     3001 atgagagcac tgtttctcat tcccagaaac cctcctcccc ggctgaagtc aaaaaaatgg
     3061 tcgaagaagt tttttagttt tatagaaggg tgcctggtga agaattacat gcagcggccc
     3121 tctacagagc agcttttgaa acatcctttt ataagggatc agccaaatga aaggcaagtt
     3181 agaatccagc ttaaggatca tatagatcgt accaggaaga agagaggcga gaaagatgaa
     3241 actgagtatg agtacagtgg gagtgaggaa gaagaggagg aagtgcctga acaggaagga
     3301 gagccaagtt ccattgtgaa cgtgcctggt gagtctactc ttcgccgaga tttcctgaga
     3361 ctgcagcagg agaacaagga acgttccgag gctcttcgga gacaacagtt actacaggag
     3421 caacagctcc gggagcagga agaatataaa aggcaactgc tggcagagag acagaagcgg
     3481 attgagcagc agaaagaaca gaggcgacgg ctagaagagc aacaaaggag agagcgggaa
     3541 gctagaaggc agcaggaacg tgaacagcga aggagagaac aagaagaaaa gaggcgtcta
     3601 gaggagttgg agagaaggcg caaagaagaa gaggagagga gacgggcaga agaagaaaag
     3661 aggagagttg aaagagaaca ggagtatatc aggcgacagc tagaagagga gcagcggcac
     3721 ttggaagtcc ttcagcagca gctgctccag gagcaggcca tgttactgga gtgccgatgg
     3781 cgggagatgg aggagcaccg gcaggcagag aggctccaga ggcagttgca acaagaacaa
     3841 gcatatctcc tggacctaca gcatgaccat aggaggccgc acccgcagca cgaccagcag
     3901 ccgccaccac cgcagcagga aagggacaag ccaagcttcc atgctcccga gcccaaagcc
     3961 cactacgagc ctgctgaccg agcgcgagag gtggaagata gatttaggaa aactaaccac
     4021 agctcccctg aagcccagtc taagcagaca ggcagagtat tggagccacc agtgccttcc
     4081 cgatcagagt ctttttccaa tggcaactcc gagtctgtgc atcccgccct gcagagacca
     4141 gcggagccac aggtacagtg gtcccacctg gcatctctca agaacaatgt ttcccctgtc
     4201 tcgcgatccc atgacttcag tgacccttct cccaaatttg cacaccacca tcttcgttct
     4261 caggacccat gtccaccttc ccgcagtgag gtgctcagtc agagctctga ctctaagtca
     4321 gaggcgcctg accctgacca aaaggcttgg tctagatcag acagtgacga ggtgcctcca
     4381 agggttcctg tgagaacaac atctcgctcc cctgttctgt cccgtcgaga ttccccactg
     4441 cagggcagtg ggcagcagaa tgaccaggca ggacagagaa actccaccag cgacattgag
     4501 cccaggcttc tgtgggagag agtggagaag ctggtgccca gacctggcag tggcagctcc
     4561 tcagggtcca gcaactcagg atcccagccc gggtctcacc ctgggtctca gagtggctcc
     4621 ggggaacgct tcagagtgag atcatcatcc aagtctgaag gctctccatc tcagcgcctg
     4681 gaaaatgcag tgaaaaaacc tgaagataaa aaggaagttt tcagacccct caagcctgct
     4741 ggcgaagtgg atctggacgc actggccaaa gagcttcgag cagtggaaga tgtacggcca
     4801 cctcacaaag taacggacta ctcctcatcc agtgaggagt cggggacgac ggatgaggag
     4861 gacgacgatg tggagcagga aggggctgac gagtccacct caggaccaga ggacaccaga
     4921 gcagcggact ctctgaattt gagcaatggt gaaacggaat ctgtgaaaac catgattgtc
     4981 catgatgatg tagaaagtga gccggccatg accccatcca aggagggcac tctaatcgtc
     5041 cgccagagta cagttgacca aaagcgtgcc agccatcatg agagcaatgg ctttgccggt
     5101 cgcattcacc tcttgccaga tctcttacag caaagccatt cctcctccac ttcctccacc
     5161 tcctcctccc catcctccag ccagccgaca cccaccatgt ccccacagac accccaggac
     5221 aagctcactg ctaatgagac tcagtccgct agtagcacac tccagaaaca caaatcttcc
     5281 tcctccttta caccttttat agaccccaga ttactacaga tttctccatc tagcggaaca
     5341 acagtgacat ctgtggtggg attttcctgt gatgggatga gaccagaagc cataaggcaa
     5401 gatcctaccc ggaaaggcga cgtggtcaat gtgaatccta ccaacactag gccacagagt
     5461 gacaccccgg agattcgtaa atacaagaag aggtttaact ctgagattct gtgtgctgcc
     5521 ttatggggag tgaatttgct agtgggtaca gagagtggcc tgatgctgct ggacagaagt
     5581 ggccaaggga aggtctatcc tcttatcaac cgaagacgat ttcaacaaat ggacgtactt
     5641 gagggcttga atgtcttggt gacaatatct ggcaaaaagg ataagttacg tgtctactat
     5701 ttgtcctggt taagaaataa aatacttcac aatgatccag aagttgagaa gaagcaggga
     5761 tggacaaccg taggggattt ggaaggatgt gtacattata aagttgtaaa atatgaaaga
     5821 atcaaatttc tggtgattgc tttgaagagt tctgtggaag tctatgcgtg ggcaccaaag
     5881 ccatatcaca aatttatggc ctttaagtca tttggagaat tggtacataa gccattactg
     5941 gtggatctca ctgttgagga aggccagagg ttgaaagtga tctatggatc ctgtgctgga
     6001 ttccatgctg ttgatgtgga ttcaggatca gtctatgaca tttatctacc aacacatatc
     6061 cagtgtagca tcaaacccca tgcaatcatc atcctcccca atacagatgg aatggagctt
     6121 ctggtgtgct atgaagatga gggggtttat gtaaacacat atggaaggat caccaaggat
     6181 gtagttctac agtggggaga gatgcctaca tcagtagcat atattcgatc caatcagaca
     6241 atgggctggg gagagaaggc catagagatc cgatctgtgg aaactggtca cttggatggt
     6301 gtgttcatgc acaaaagggc tcaaagacta aaattcttgt gtgaacgcaa tgacaaggtg
     6361 ttctttgcct ctgttcggtc tggtggcagc agtcaggttt atttcatgac cttaggcagg
     6421 acttctcttc tgagctggtg aacccagctt tcttgtacaa agtggtgata atcgaattcc
     6481 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6541 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6601 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6661 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6721 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6781 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6841 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     6901 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6961 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7021 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa
     7081 ttcccgcggt tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     7141 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     7201 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     7261 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     7321 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     7381 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     7441 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     7501 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     7561 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctcacg tggccaccat
     7621 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     7681 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     7741 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     7801 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     7861 ggaatttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     7921 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     7981 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     8041 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     8101 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     8161 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     8221 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     8281 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     8341 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     8401 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     8461 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     8521 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     8581 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     8641 atagggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8701 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8761 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8821 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8881 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8941 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     9001 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     9061 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9121 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     9181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9301 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     9361 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9421 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9481 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     9541 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9601 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9661 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9721 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9781 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9841 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9901 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     9961 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    10021 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    10081 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    10141 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    10201 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    10261 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    10321 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    10381 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    10441 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    10501 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    10561 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    10621 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    10681 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    10741 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    10801 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    10861 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    10921 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    10981 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    11041 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    11101 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    11161 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    11221 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    11281 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    11341 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    11401 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    11461 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11521 tacagcgtga gctatgagaa agcgccacgc ttcccgaaga gagaaaggcg gacaggtatc
    11581 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11641 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11701 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11761 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    11821 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    11881 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    11941 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    12001 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    12061 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    12121 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    12181 tggagctgca agctt
//
