LOCUS       sequence-456149-        8853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: HA-MST1
FEATURES             Location/Qualifiers
     source          1..8853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2149..2175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2179..2205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3685..4259,4260..4262,4263..4271)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3852..3869)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4079..4098
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    4710..5298
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4763..4783)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5181..5192)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5370..5603
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5681..5802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5718..5737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5772..5791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5842..5977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5997..6016)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5998..6016)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6026..6043)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6026..6042)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6035..6057)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6184..6639
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6271..6290)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6481..6502
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6665..6769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6770..6838,6839..7630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6988..7007)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7801..8389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8290..8309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8543..8560
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8677..8698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8713..8718,8719..8736,8737..8743)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8751..8767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8756..8778
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8775..8791
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8775..8791
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8810..8830
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8812..8830
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgta cccctacgac
     2161 gtgcccgact acgccggcta tccgtatgat gtcccggact atgcaggaag cggagagacg
     2221 gtacagctga ggaacccgcc gcgccggcag ctgaaaaagt tggatgaaga tagtttaacc
     2281 aaacaaccag aagaagtatt tgatgtctta gagaaacttg gagaagggtc ctatggcagc
     2341 gtatacaaag ctattcataa agagaccggc cagattgttg ctattaagca agttcctgtg
     2401 gaatcagacc tccaggagat aatcaaagaa atctctataa tgcagcaatg tgacagccct
     2461 catgtagtca aatattatgg cagttatttt aagaacacag acttatggat cgttatggag
     2521 tactgtgggg ctggttctgt atctgatatc attcgattac gaaataaaac gttaacagaa
     2581 gatgaaatag ctacaatatt acaatcaact cttaagggac ttgaatacct tcattttatg
     2641 agaaaaatac accgagatat caaggcagga aatattttgc taaatacaga aggacatgca
     2701 aaacttgcag attttggggt agcaggtcaa cttacagata ccatggccaa gcggaataca
     2761 gtgataggaa caccattttg gatggctcca gaagtgattc aggaaattgg atacaactgt
     2821 gtagcagaca tctggtccct gggaataact gccatagaaa tggctgaagg aaagccccct
     2881 tatgctgata tccatccaat gagggcaatc ttcatgattc ctacaaatcc tcctcccaca
     2941 ttccgaaaac cagagctatg gtcagataac tttacagatt ttgtgaaaca gtgtcttgta
     3001 aagagccctg agcagagggc cacagccact cagctcctgc agcacccatt tgtcaggagt
     3061 gccaaaggag tgtcaatact gcgagactta attaatgaag ccatggatgt gaaactgaaa
     3121 cgccaggaat cccagcagcg ggaagtggac caggacgatg aagaaaactc agaagaggat
     3181 gaaatggatt ctggcacgat ggttcgagca gtgggtgatg agatgggcac tgtccgagta
     3241 gccagcacca tgactgatgg agccaatact atgattgagc acgatgacac gttgccatca
     3301 caactgggca ccatggtgat caatgcagag gatgaggaag aggaaggaac tatgaaaaga
     3361 agggatgaga ccatgcagcc tgcgaaacca tcctttcttg aatattttga acaaaaagaa
     3421 aaggaaaacc agatcaacag ctttggcaag agtgtacctg gtccactgaa aaattcttca
     3481 gattggaaaa taccacagga tggagactac gagtttctta agagttggac agtggaggac
     3541 cttcagaaga ggctcttggc cctggacccc atgatggagc aggagattga agagatccgg
     3601 cagaagtacc agtccaagcg gcagcccatc ctggatgcca tagaggctaa gaagagacgg
     3661 caacaaaact tctgagcaat tccgcccctc tccctccccc ccccctaacg ttactggccg
     3721 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3781 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3841 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     3901 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3961 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4021 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4081 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4141 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4201 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4261 tggccacaac catgaagacc ttcaacatct ctcagcagga ccttgagctg gtggaagttg
     4321 ccactgagaa aatcaccatg ctctatgagg acaacaagca ccatgttggt gctgccatca
     4381 ggaccaagac aggagaaatc atctctgctg tccacattga agcctacatt ggcagggtca
     4441 ctgtgtgtgc agaggccatt gccattgggt ctgcagtctc caatgggcag aaggactttg
     4501 acaccattgt ggctgtgagg cacccctaca gtgatgaggt ggacaggtcc atcagagtgg
     4561 tgtccccctg tggcatgtgc agggagctca tctcagacta tgcccctgat tgctttgttc
     4621 tgattgagat gaatggcaag ctggtcaaga caaccattga ggagctgatt ccactgaaat
     4681 acaccagaaa ctaaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     4741 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4801 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4861 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4921 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4981 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5041 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5101 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5161 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5221 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5281 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     5341 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5401 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5461 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5521 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5581 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     5641 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     5701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5821 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5881 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5941 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     6001 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6061 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6121 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6181 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6241 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6301 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6361 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6421 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6481 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6541 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6601 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6661 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6721 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6781 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6841 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6901 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6961 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7021 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7081 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7141 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7201 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7261 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7321 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7381 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7441 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7501 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7561 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7621 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7681 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7741 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7801 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7861 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7921 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7981 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8041 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8101 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8161 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8221 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8281 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8341 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8401 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8461 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8521 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8581 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8641 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8701 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8761 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     8821 tcactaaagg gaacaaaagc tggagctgca agc
//
